Electron Microscopic Studies of Biological Reactions. I. Reduction of Potassium Tellurite by Corynebacterium diphtheriae.

Morton, Harry E.; Anderson, Thomas F.

doi:10.3181/00379727-46-11963

Cited by 31 publications

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“…for instance, mycobacteria (Lembke and Ruska, 1940; Knaysi et al, 1950;Mudd et al, 1951b), C. diphtheriae (Morton and Anderson, 1941;K6nig and Wsinklei, 1948;Bringmann, 1950Bringmann, -1951, Staphylococcuis flaomcyaneuis and iVeisseria m2eningitidis (Knaysi and Mudd, 1943), and Bacillus mycoides (Knaysi and Bakei-, 1947). Konig and Winkler pioved the identity of the electron-scattering granules with the metachromatic volutin granules by examining the same Neisser stained film first by the light microscope and then by the electron microscope.…”

Section: [Vol 68mentioning

confidence: 99%

Volutin Production in Aerobacter Aerogenes Due to Nutrient Imbalance

1954

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Metachromatically staining granules of volutin, first observed in C(oryrebacterium xerosis and Corynebacterium diphtheriae, have since been demonstrated in a wide variety of bacteria, yeasts, fungi, and algae. In the species normally forming volutin, the amount produced is influenced greatly by the conditions of culture. Moreover, there are some species which form the granules only in exceptional circumstances. MATERIALS AND METHODS The observations were made with a capsulate, noumotile strain (A3) of A. aerogenes which had the following properties: methyl-red negative, producing acetyimethylcarbinol, utilizing citrate, not producing indole, not liquefying gelatin, and fermenting glucose, lactose, sucrose, mannitol, inositol, and glycerol with production of acid and gas. Surface cultures were grown on 50 ml amounts of agar medium in 9 cm petri dishes. A "complete" medium was used to give growths limited by exhaustion of the carbon and energy source (glucose); the cells so grown are termed "carbondeficient." This medium contained per 100 ml distilled water: glucose, 0.2 g; phosphate (mixture of 3 parts by weight Na2HPO4 and 1 part NaH2PO4; pH-7.3), 1.0 g; NH4C1, 0.1 g; NaCl, 0.2 g; NaS,04, 0.01 g; KCI, 0.01 g; MgC12, 40

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Section: [Vol 68mentioning

confidence: 99%

Volutin Production in Aerobacter Aerogenes Due to Nutrient Imbalance

1954

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“…particles of influenza to be only about11 millimicrons in diameter. Subsequent work, however, revealed an unreckoned complication dependent on convection currents in the angle centrifuge.56 Critical reinvestigations of the infectious units of human influenza viruses A and B by Taylor, Sharp and their collaborators,57 by Stanley 58 and by Friedewald and Pickels 59 have recently indicated in each case that the unit infectious particles of human influenza virus have diameters within the range originally estimated.…”

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Pathogenic Bacteria, Rickettsias and Viruses as Shown by the Electron Microscope

Mudd¹

1944

JAMA

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“…Many microorganisms produce black intracellular deposits when grown in media supplemented with potassium tellurite (14,15,23,25,(28)(29)(30)(31). Even sensitive bacteria are capable of forming jet-black colonies when growing at subinhibitory tellurite concentrations (25).…”

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Purification and biochemical characterization of tellurite-reducing activities from Thermus thermophilus HB8

et al. 1988

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Cell-free extracts of Thermus thermophilus HB8 catalyze the in vitro, NADH-dependent reduction of potassium teilurite (K2TeO3). Three different protein fractions with tellurite-reducing activities were identified.Two exhibited high molecular weight and were composed of at least two different polypeptides. The protein in the third fraction was purified to homogeneity and had a single polypeptide chain of 53 to 54 kilodaltons, with an isoelectric point of 8.1. Each enzyme was thermostable, the temperature optimum was 75°C, and 30 mM NaCl, 1.5 M urea, or 0.004% sodium dodecyl sulfate caused 50% inhibition of the enzymes. However, 2% Triton X-100 did not have an inhibitory effect. The enzymes were also able to catalyze the reduction of sodium selenite and sodium sulfite in vitro. NADH was replaceable by NADPH. Divalent cations, such as Ca2' and

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Electron Microscopic Studies of Biological Reactions. I. Reduction of Potassium Tellurite by Corynebacterium diphtheriae.

Abstract: 1. The presence of reversibly oxidizahle substances in melano. blasts that accelerate oxidatioii o f dopa by atmospheric aiid dissolved oxygen.

Cited by 31 publications

References 0 publications

Volutin Production in Aerobacter Aerogenes Due to Nutrient Imbalance

Volutin Production in Aerobacter Aerogenes Due to Nutrient Imbalance

Pathogenic Bacteria, Rickettsias and Viruses as Shown by the Electron Microscope

Purification and biochemical characterization of tellurite-reducing activities from Thermus thermophilus HB8

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