Electron Microscopic Methods in Membrane Biology

Zingsheim, H. P.; Plattner, Helmut

doi:10.1007/978-1-4757-5820-7_1

Cited by 40 publications

(31 citation statements)

References 493 publications

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“…These findings show that the described fine-lamellar electron-dense structures of about 5 nm periodicity, emerging after different types of peroxidation, are promoted by the peroxidative damage of the ROS and possibly represent specific sites of peroxidation as proposed by Armstrong and Hiramitsu [5]. Postfixation with osmium tetroxide stains all unsaturated lipids and produces a picture similar to the specific staining with TMB [96], but the emergence of the fine-lamellar structures is strongly enhanced by the use of TMB. Since TMB is able to react with hydroperoxides [75,78,85], it would appear that the peroxidative property of the detected structures [5] causes this effect, but a satisfactory explanation is still elusive and further investigation is required.…”

Section: Discussionsupporting

confidence: 66%

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Tracing of benzidine-reactive substances in ROS, RPE and choroid after light-induced peroxidation

Kayatz¹,

Heimann²,

Schraermeyer³

1999

Graefe's Archive for Clinical and Experimental Ophthalmology

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Light conditions that trigger the disc shedding possibly activate a mechanism to extrude peroxidative damaged material over the complete ROS into the extracellular space to diminish peroxidative damage inside the ROS. Indigestible residual material from the ROS degradation in the phagosomes consists of membranous lipids associated with peroxidative damaged proteins. The residual material seems to be transported through Bruch's membrane into the choriocapillaris.

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Section: Discussionsupporting

confidence: 66%

“…The fine-lamellar structures described by Rungger-Brändle and co-workers [70] were shown to have a higher share of saturated lipids that could not be fixed by osmium tetroxide [96]. Thus peroxidation leads to a higher share of saturated fatty acids that can be fixed neither by glutaraldehyde nor by osmium tetroxide.…”

Section: Discussionmentioning

confidence: 98%

Tracing of benzidine-reactive substances in ROS, RPE and choroid after light-induced peroxidation

Kayatz¹,

Heimann²,

Schraermeyer³

1999

Graefe's Archive for Clinical and Experimental Ophthalmology

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“…3a). The trilaminar structure of the limiting membrane of the vesicles was characteristic of osmium-fixed bilayer membranes (35). Residual cells and large cell envelope fragments present in the crude supernatants (data not shown) were completely removed by the filtration step without any significant loss of ice nucleation activity.…”

Section: Methodsmentioning

confidence: 87%

Release of cell-free ice nuclei by Erwinia herbicola

Phelps¹,

Giddings²,

Prochoda³

et al. 1986

J Bacteriol

125

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Several ice-nucleating bacterial strains, including Erwinia herbicola, Pseudomonas fluorescens, and Pseudomonas syringae isolates, were examined for their ability to shed ice nuclei into the growth medium. Only E. herbicola isolates shed cell-free ice nuclei active at -2 to -10°C. These cell-free nuclei exhibited a freezing spectrum similar to that of ice nuclei found on whole cells, both above and below -5°C. Partially purified cell-free nuclei were examined by density gradient centrifugation, chemical and enzymatic probes, and electron microscopy. Ice-nucleating activity in these cell-free preparations was associated with outer membrane vesicles shed by cells and was sensitive to protein-modifying reagents.

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“…Small pieces of the human retina and choroid were dissected at the posterior pole <2 min after removal of the eyeball and fixed at 4˚C in 2% buffered glutaraldehyde for 2 h and postfixed in 2% osmium tetroxide for another 2 h. The postfixation with osmium tetroxide demonstrates lipid peroxides as effectively as tetramethylbenzidine but avoids incubation, which might damage membrane structures (68). The specimens were dehydrated, embedded in Araldite, sectioned with a Reichert ultramicrotome (Reichert, Vienna, Austria), contrasted with lead citrate and uranyl acetate and studied with a Zeiss 109 electron microscope.…”

Section: Methodsmentioning

confidence: 99%

Retinal pigment epithelium, age-related macular degeneration and neurotrophic keratouveitis

Bianchi

Scarinci

Ripandelli

et al. 2012

International Journal of Molecular Medicine

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Abstract. Age-related macular degeneration (AMD) is the leading cause of impaired vision and blindness in the aging population. The aims of our studies were to identify qualitative and quantitative alterations in mitochondria in human retinal pigment epithelium (RPE) from AMD patients and controls and to test the protective effects of pigment epithelium-derived factor (PEDF), a known neurotrophic and antiangiogenic substance, against neurotrophic keratouveitis. Histopathological alterations were studied by means of morphometry, light and electron microscopy. Unexpectedly, morphometric data showed that the RPE alterations noted in AMD may also develop in normal aging, 10-15 years later than appearing in AMD patients. Reduced tear secretion, corneal ulceration and leukocytic infiltration were found in capsaicin (CAP)-treated rats, but this effect was significantly attenuated by PEDF. These findings suggest that PEDF accelerated the recovery of tear secretion and also prevented neurotrophic keratouveitis and vitreoretinal inflammation. PEDF may have a clinical application in inflammatory and neovascular diseases of the eye.

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Electron Microscopic Methods in Membrane Biology

Cited by 40 publications

References 493 publications

Tracing of benzidine-reactive substances in ROS, RPE and choroid after light-induced peroxidation

Tracing of benzidine-reactive substances in ROS, RPE and choroid after light-induced peroxidation

Release of cell-free ice nuclei by Erwinia herbicola

Retinal pigment epithelium, age-related macular degeneration and neurotrophic keratouveitis

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