Electron-microscopic immunocytochemical study of the endocrine pancreas of sea bass (Dicentrarchus labrax)

Agulleiro, B.; Lozano, Marı́a Teresa; Abad, María Eugenia; Hernández, M. P. García

doi:10.1007/bf00318749

Cited by 22 publications

(20 citation statements)

References 51 publications

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“…Therefore, we are inclined at this point to view the cell of the bowfin pancreas containing rod-shaped inclusions as an A cell. It is not uncommon for glucagon and pancreatic polypeptide to be localised within the same peripherally located islet cells in fish (Abad et al, 1988;Lozano et al, 1991;Agulleiro et al, 1993;Youson, 1998, 1999), and this definitely was the case in bowfin. Therefore, we cannot exclude the possibility that the cells containing rod-shaped inclusions might not fall within this same category of A/F cell type, like that described for gar (Groff and Youson, 1998).…”

Section: Discussionmentioning

confidence: 98%

“…The D cells could be clearly distinguished from the B cells by their large, irregularly shaped, electron-dense granules with a tightfitting membrane. However, immunocytochemistry at the electron microscope level is required for definitive identification of D cells, and to establish whether their granules colocate different SST antisera (Agulleiro et al, 1993;Al-Mahrouki and Youson, 1999).…”

Section: Discussionmentioning

confidence: 99%

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The endocrine cells in the gastroenteropancreatic system of the bowfin, Amia calva L.: An immunohistochemical, ultrastructural, and immunocytochemical analysis

Youson¹,

Al‐Mahrouki²,

Naumovski³

et al. 2001

Journal of Morphology

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The gastroenteropancreatic (GEP) endocrine system of bowfin (Amia calva) was described using light and electron microscopy and immunological methods. The islet organ (endocrine pancreas) consists of diffusely scattered, mostly small islets and isolated patches of cells among and within the exocrine acini. The islets are composed of abundant, centrally located B cells immunoreactive to bovine and lamprey insulin antisera and D cells showing a widespread distribution and specificity to somatostatin antibodies. A and F cells are present at the very periphery of the islets and are immunoreactive with antisera against glucagon (and glucagon-like peptide) and several peptides of the pancreatic polypeptide (PP)-family, respectively. The peptides of the two families usually collocates within the same peripheral islet cells and are the most common immunoreactive peptides present in the extra-islet tissue. Immunocytochemistry and fine structural observations characterised the granule morphology for B and D cells and identified two cell types with granules immunoreactive to glucagon antisera. These two putative A cells had similar granules, which were distinct from either B or D cells, but one of the cells had rodshaped cytoplasmic inclusions within cisternae of what appeared to be rough endoplasmic reticulum. The inclusions were not immunoreactive to either insulin or glucagon antisera. Only small numbers of cells in the stomach and intestine immunoreacted to antisera against somatostatin, glucagon, and PP-family peptides. The paucity of these cells was reflected in the low concentrations of these peptides in intestinal extracts. The GEP system of bowfin is not unlike that of other actinopterygian fishes, but there are some marked differences that may reflect the antiquity of this system and/or may be a consequence of the ontogeny of this system in this species.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

The endocrine cells in the gastroenteropancreatic system of the bowfin, Amia calva L.: An immunohistochemical, ultrastructural, and immunocytochemical analysis

Youson¹,

Al‐Mahrouki²,

Naumovski³

et al. 2001

Journal of Morphology

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“…The average size of B-cell granules (110 nm) in gars was less than in most other vertebrates, which ranged from an average of 165 to 622 nm (Brinn 1975;Kobayashi et al 1976;Ortiz de Zarate et al 1991;Agulleiro et al 1993), although it appears that the gar has one of the greatest ranges of granule sizes (60-470 nm). The gar had the same average size of B-cell granules as the lamprey (110 nm), but lamprey granules had a size range of only 140-200 nm .…”

Section: Discussionmentioning

confidence: 88%

“…The difference noted from other species was the wide variety of shapes of the gar B cell granule cores. Rodlike crystalloid and irregular cores were observed in the B cells of some elasmobranchs (Kobayashi and Takahashi 1970), lampreys , and teleosts (Carrillo et al 1986;Agulleiro et al 1993), although in some teleostean species, only round secretory granules could be observed (Brinn 1975;Lozano and Agulleiro 1986). Nakamura and Fig.…”

Section: Discussionmentioning

confidence: 94%

“…A large number of immunocytochemical and ultrastructural studies have been carried out on the pancreatic islet tissue of adult teleosts (for example, see Kobayashi and Takahashi 1970;Boquist and Patent 1971;Brinn 1975;Kobayashi et al 1976;Carrillo et al 1986;Lozano and Agulleiro 1986;Beccaria et al 1990;Agulleiro et al 1993), including a description of the four endocrine cell types. Detailed fine-structural study and immunocytochemistry at the ultrastructural level, however, have not been performed on the gar endocrine pancreas.…”

Section: Introductionmentioning

confidence: 99%

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Fine structure and immunocytochemistry of cells within the endocrine pancreas of the gar (Lepisosteus osseus)

Groff¹,

Youson²

1998

Can. J. Zool.

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Routine electron microscopy and immunocytochemistry were used to describe the cell types in the islets of the endocrine pancreas of the gar Lepisosteus osseus, an actinopterygian fish of the order Semionotiformes, which has an ancient lineage. The general fine-structural features of cells composing the islets reflect their synthesis and packaging of protein for liberation at their perivascular surface. Cells are directly apposed to numerous capillaries and they are richly innervated with nerve terminals containing dense-cored vesicles. The islet tissue comprises many B cells, which are easily distinguished by their ubiquitous granules with polymorphous matrix cores and a loose-fitting membrane. These granules are only immunoreactive with an insulin antiserum. Only one type of D cell is found throughout the islets and it contains many granules of varying electron density, the most abundant granule profile being dumbbell-shaped. All granules in this cell type have a tight-fitting limiting membrane and they immunostain with antisomatostatin-14 and -34. Cells at the periphery of the islet contained granules of similar morphology to those in the D cells, but the granules were less numerous. Many granules in the cells were immunoreactive with both antiglucagon and antineuropeptideY, while others immunostained with only one of these antibodies. Since no cells stained exclusively for either glucagon or neuropeptide Y, it was concluded that there are only three cell types in the endocrine pancreas of the gar: B and D cells and a third cell type (A/F) that co-localizes peptides of the glucagon and pancreatic polypeptide family. Although this co-localization is not uncommon in the vertebrate endocrine pancreas, it may have some phylogenetic and (or) ontogenetic significance in this organism.

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Immunocytochemical and ultrastructural characterization of prolactin, growth hormone, and somatolactin cells from the Mediterranean yellowtail (Seriola dumerilii, Risso 1810)

et al. 1997

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Electron-microscopic immunocytochemical study of the endocrine pancreas of sea bass (Dicentrarchus labrax)

Cited by 22 publications

References 51 publications

The endocrine cells in the gastroenteropancreatic system of the bowfin, Amia calva L.: An immunohistochemical, ultrastructural, and immunocytochemical analysis

The endocrine cells in the gastroenteropancreatic system of the bowfin, Amia calva L.: An immunohistochemical, ultrastructural, and immunocytochemical analysis

Fine structure and immunocytochemistry of cells within the endocrine pancreas of the gar (Lepisosteus osseus)

Immunocytochemical and ultrastructural characterization of prolactin, growth hormone, and somatolactin cells from the Mediterranean yellowtail (Seriola dumerilii, Risso 1810)

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