Electron microscopic analysis of sugar residues of glycoproteins in the acrosomes of spermatids using various lectins.

Yamamoto, Noboru

doi:10.1267/ahc.15.139

Cited by 19 publications

(14 citation statements)

References 32 publications

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“…These findings support the idea that acrosomal glycoconjugates could be modified during acrosome development: (1) by addition of new carbohydrates; and (2) by compression of acrosomal components that results in the masking of some sugar residues. This variability in lectin labeling has also been reported in other species, including another amphibian, Bufo calamita (Ballesta et al, 1991); however, most of the acrosomal glycoconjugate variabilities reported during spermiogenesis are in mammals Vanha-Perttula, 1984, 1986a;Ertl and Wrobel, 1992;Jones et al, 1992a,b;Kurohmaru et al, 1995Kurohmaru et al, , 1996Söderström et al, 1984;Yamamoto, 1982). The high variability in the species studied, methods employed, and lectins tested in these reports make it impossible to analyze if a possible common pattern of glycosylation or glycoconjugate organization exists during acrosome development.…”

Section: Lectin Histochemistry Of Spermiogenic (Haploid) Germ Cellssupporting

confidence: 57%

Glycoconjugates of the urodele amphibian testis shown by lectin cytochemical methods

Sáez

Madrid

Cardoso

et al. 2004

Microscopy Res & Technique

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Lectin histochemistry is a useful method that allows the in situ identification of the terminal sugar moieties of the carbohydrates that form the glycoconjugates. Moreover, when it is combined with chemical or enzymatic deglycosylation pretreatments, lectin histochemistry can be employed to determine if carbohydrates are linked to the protein core by means of an N- or O-glycosidic linkage or, indeed, to partially sequence the sugar chains. One of the most interesting model organs for the study of spermatogenesis is the amphibian urodele testis. However, this organ has not been very widely investigated with lectin histochemical research. In the last few years, we have carried out a research project to identify and locate glycoconjugates in the testis of the urodele Pleurodeles waltl, the Spanish newt, as a first approach to identify possible carbohydrates with key roles in spermatogenesis. Our findings reveal some glycan chains located in a fusome-like structure in early (diploid) germ cells, oligosaccharides with terminal GalNAc in the acrosome, the occurrence of glycan modifications in the acrosomal contents during spermiogenesis, and changes in glycan composition of follicle and interstitial cells during the spermatogenetic cycle. Furthermore, the similar labeling pattern of follicle and duct cells supports the hypothesis for a common origin of both cell types.

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Section: Lectin Histochemistry Of Spermiogenic (Haploid) Germ Cellssupporting

confidence: 57%

Glycoconjugates of the urodele amphibian testis shown by lectin cytochemical methods

Sáez

Madrid

Cardoso

et al. 2004

Microscopy Res & Technique

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“…Therefore, such differences in lectin bindings in the acrosomal region may reflect different acrosomal components of sugar residues in each mammalian species. In contrast to the variation in lectin bindings in acrosome, PNA had a conserved binding pattern in the acrosomal region of all mammalian species examined so far [1][2][3][4][5][6][7][8][9][10][11][12][13]. In concurrence, the present study also showed an intense reaction in the acrosomal region of bats.…”

Section: Discussionsupporting

confidence: 75%

“…These findings, therefore, suggest that PSA may detect some specific glycoconjugates in a certain organelle of the Japanese lesser horseshoe bats. The UEA-I reaction, representing the appearance of α-Fucose residues, have been reportedly detected in the acrosomal region of the rat [5,6], mouse [7,8], nutria [8] and bull [12], but no reaction was or has previously been observed in the testes of the Java fruit bat, the Japanese lesser horseshoe bat, the guinea pig [9] or the musk shrew [10]. It has been demonstrated that SBA reacts strongly in the acrosomal region of many mammalian species, such as man [1][2][3][4], rat [5,6], mouse [7,8], nutria [8], guinea pig [9], common tree shrew [11], bull [12] and shiba goat [13], but this reaction could not be detected in either the Java fruit bat or the Japanese lesser horseshoe bat.…”

Section: Discussionmentioning

confidence: 99%

“…Until now, several studies have been done on lectin-binding patterns in the testes of mammalian species, such as the man [1][2][3][4], rodents [5][6][7][8][9], insectivore [10], common tree shrew [11], bull [12] and goat [13], but, there are no reports on the Order Chiroptera. In this study, two kinds of bats were selected: one (Java fruit bat) belongs to the Suborder Megachiroptera, and the other (Japanese lesser horseshoe bat) belongs to the Suborder Microchiroptera.…”

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confidence: 99%

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Lectin-Binding Patterns in the Testes of the Java Fruit Bat (Pteropus vampyrus) and the Japanese Lesser Horseshoe Bat (Rhinolophus cornutus).

Morigaki

Kurohmaru

Kanai

et al. 2000

Journal of Reproduction and Development

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Abstract. Lectin binding patterns in the testes of the Java fruit bat, Pteropus vampyrus, and the Japanese lesser horseshoe bat, Rhinolophus cornutus, were investigated by light microscopy. Binding patterns were similar in both species, except for some slight differences. UEA-I, SBA, DBA and BSL-I revealed no reaction in the testes of either species. Con A and WGA gave a diffuse reaction all over the seminiferous epithelium in both species. In addition to this binding pattern, PHA-E exhibited a granular reaction within the cytoplasm of pachytene spermatocytes. PNA and RCA-I gave an intense reaction in the acrosomal region from Golgi to acrosome-phase spermatids in both species, but, in addition to this binding pattern, RCA-I reacted in the cytoplasm of spermatocytes and spermatids in the Java fruit bats. PSA revealed a granular reaction in the cytoplasm of spermatids in the Japanese lesser horseshoe bats. These binding patterns were similar to those of mammals studied before, except that a few specific bindings were detected in the bats.

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“…In these reports, Lowicryl K4M provided adequate tissue preservation and, when combined with lectin-gold cytochemistry, allowed precise localization of intracellular and extracellular glycoconjugates at the ultrastructural level. In contrast to epoxy resins, Lowicryl K4M produces low background and low nonspecific labeling (Roth, 1983), and does not require etching steps that may result in a loss of tissue glycoconjugates (Suzuki et al, 1991;Yamamoto, 1982).…”

Section: Methodological Considerationsmentioning

confidence: 99%

Pilocarpine-induced changes in the saccharide composition of the tectorial membrane and interdental cells of the organ of Corti: a study with gold-labeled lectins.

Rubio¹,

Rueda²,

Prieto³

et al. 1994

J Histochem Cytochem.

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The glycoconjugates in the cytoplasm of inner ear interdental cells and those constituting the limbal tectorial membrane were identified by a post-embedding cytochemical method using low-temperature embedding in hwiayl K4M and labeling with biotinylated lectins, goat anti-biotin antibody, rabbit anti-goat antibody, and gold-labeled protein A in control animals, and after the systemic injection of pilocarpine. The lectins used were ConA, PHA-E, PSA, RCA, SBA, Sua-WGA, UEA, and WGA. In control animals, a semiquantitive analysis of gold particles showed that Succ-WGA produced the strongest labeling on the tectorial membrane, followed by SBA, ConA, WGA, RCA, PHA-E, and PSA. The lowest values were obtained with UEA. The cytoplasm of the interdental cells was also labeled with all the lectins, but the number of particles/pn' was lower than

show abstract

Electron microscopic analysis of sugar residues of glycoproteins in the acrosomes of spermatids using various lectins.

Cited by 19 publications

References 32 publications

Glycoconjugates of the urodele amphibian testis shown by lectin cytochemical methods

Glycoconjugates of the urodele amphibian testis shown by lectin cytochemical methods

Lectin-Binding Patterns in the Testes of the Java Fruit Bat (Pteropus vampyrus) and the Japanese Lesser Horseshoe Bat (Rhinolophus cornutus).

Pilocarpine-induced changes in the saccharide composition of the tectorial membrane and interdental cells of the organ of Corti: a study with gold-labeled lectins.

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