Electron Microscope Studies of Ascites Tumor Cells

Selby, Cecily Cannan; Biesele, John J.; Grey, Clifford E.

doi:10.1111/j.1749-6632.1956.tb50891.x

Cited by 63 publications

(7 citation statements)

References 30 publications

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“…The over-all picture of fine structural organisation reported here for the HeLa cells is in accordance with that commonly present in undifferentiated malignant cells of various types (26,19,30,31). However, three important and unusual features have been found and call for comment.…”

Section: Discussionsupporting

confidence: 86%

SOME UNUSUAL FEATURES OF FINE STRUCTURE OBSERVED IN HELA CELLS

Epstein

1961

The Journal of Cell Biology

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HeLa cells from conventional culture media have been studied in thin sections with the electron microscope; in many cases cells were examined in sets of sections cut in series. The fine structure of the cells is described including three unusual features not hitherto reported. It has been found that numerous cells contained rows of parallel smooth surfaced cisternae spaced about 150 m# apart and communicating with rough surfaced elements of the endoplasrnic reticulum. These cisternae resembled "annulate lamellae" but did not contain regular arrays of pores. In many cells an area of juxtanuclear cytoplasm was occupied by a membranous structure composed of closely applied pairs of narrow cisternae either arranged in concentric rings or else extending in several directions in a haphazard manner. Sparse particles were present on the outer membranes of each pair of cisternae. Communications between the double cisternae and other membrane-bounded structures were not observed. A small number of cells contained areas of cytoplasm devoid of organelles and filled with amorphous fuzzy material. The observations recorded are discussed.

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Section: Discussionsupporting

confidence: 86%

SOME UNUSUAL FEATURES OF FINE STRUCTURE OBSERVED IN HELA CELLS

Epstein

1961

The Journal of Cell Biology

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“…Many of the salient features of a typical Ehlich cell have been described by others (6). These are illustrated in Fig.…”

Section: Observationsmentioning

confidence: 78%

An Electron Microscope Study of the Morphology and Distribution of the Intracytoplasmic "Virus-Like" Particles of Ehrlich Ascites Tumor Cells

Adams

Prince

1957

The Journal of Cell Biology

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Electron microscope studies of eight different sublines of Ehrlich ascites tumor cells which had not, as far as could be determined, come in contact with any known virus, revealed dense particles measuring approximately 55 to 70 mµ in diameter, both within and attached to the wall of cytoplasmic vesicles identified as the endoplasmic reticulum. All tumor sublines contained significant numbers of particles and revealed no qualitative or quantitative differences in particle morphology or distribution. It is concluded that these structures are a constant morphological component of the Ehrlich ascites tumor and that they probably do not represent contaminating virus. Their morphology and distribution are described, and the possible interpretations of their significance are discussed.

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“…Each of these tissues has a different proportion of RER to SER as determined by electron microscopy of unfractionated tissue; the majority of the ascites ER is in a smooth configuration (9), the ER of the adrenal cortex is essentially all smooth (10,11) and that of rat liver contains equivalent proportions of RER and SER.…”

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confidence: 99%