Electron density imaging of protein films on gold-particle surfaces with transmission electron microscopy

Jürgens, Ludger; Nichtl, Alfons; Werner, Ulf

doi:10.1002/(sici)1097-0320(19991001)37:2<87::aid-cyto1>3.0.co;2-1

Cited by 26 publications

(10 citation statements)

References 16 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to AuNPs, the AuNPs/jacalin-FITC nanoconjugate presented a shadow around the particle surface, which may be an indicative of the formation of a protein layer around the AuNPs. This phenomenon has been observed by other authors in the study of protein-conjugated nanoparticles [12,33,34].…”

Section: Incubation Of the Cells With The Nanoconjugatesupporting

confidence: 81%

Synthesis and characterization of jacalin-gold nanoparticles conjugates as specific markers for cancer cells

Marangoni¹,

Paino²,

Zucolotto³

2013

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

Section: Incubation Of the Cells With The Nanoconjugatesupporting

confidence: 81%

Synthesis and characterization of jacalin-gold nanoparticles conjugates as specific markers for cancer cells

Marangoni¹,

Paino²,

Zucolotto³

2013

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

“…TEM image indicates that the colloid gold particles were almost of the same diameter and aggregated free. Other researchers usually characterized gold particles with bound antibody by TEM using a contrasting agent such as uranyl acetate or digital contrast enhancement [23], [25]–[27]. In the present study, the difference between gold particles before and after absorption of antibody was obvious in the TEM figures although without contrasting agent or technique.…”

Section: Discussionmentioning

confidence: 45%

Preparation of Colloidal Gold Immunochromatographic Strip for Detection of Paragonimiasis skrjabini

Wang

Zhang

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

BackgroundParagonimiasis is a food-borne trematodiasis, a serious public health issue and a neglected tropical disease. Paragonimus skrjabini is a unique species found in China. Unlike paragonimiasis westermani, it is nearly impossible to make a definitive diagnosis for paragonimiasis skrjabini by finding eggs in sputum or feces. Immunodiagnosis is the best choice to detect paragonimiasis skrjabini. There is an urgent need to develop a novel, rapid and simple immunoassay for large-scale screening patients in endemic areas.Methodology/Principal FindingsTo develop a rapid, simple immunodiagnostic assay for paragonimiasis, rabbit anti-human IgG was conjugated to colloidal gold particles and used to detect antibodies in the sera of paragonimiasis patients. The synthesis and identification of colloidal gold particles and antibody-colloidal gold conjugates were performed. The size of colloidal gold particles was examined using a transmission electron microscope (TEM). The average diameter of colloidal gold particles was 17.46 nm with a range of 14.32–21.80 nm according to the TEM images. The formation of antibody-colloidal gold conjugates was monitored by UV/Vis spectroscopy. Excretory-secretory (ES) antigen of Paragonimus skrjabini was coated on nitrocellulose membrane as the capture line. Recombinant Staphylococcus protein A was used to prepare the control line. This rapid gold immunochromatographic strip was assembled in regular sequence through different accessories sticked on PVC board. The relative sensitivity and specificity of the strip was 94.4% (51/54) and 94.1% (32/34) respectively using ELISA as the standard method. Its stability and reproducibility were quite excellent after storage of the strip at 4°C for 6 months.Conclusions/SignificanceImmunochromatographic strip prepared in this study can be used in a rapid one-step immunochromatographic assay, which is instantaneous and convenient.

show abstract

“…High resolution TEM images of AuNPs coated with peptide showed a “halo” layer surrounding the surface of the nanoparticles indicating that coating of the gold with the peptide had occurred and this helped to confirm the peptide coating to AuNPs. The peptide coats the AuNPs as a monolayer with a sharp border with constant thickness on the gold nanoparticle surface[32].…”

Section: Discussionmentioning

confidence: 99%

Novel screening test for celiac disease using peptide functionalised gold nanoparticles

Kaur

Shimoni

Wallach

2018

WJG

View full text Add to dashboard Cite

AIMTo develop a screening test for celiac disease based on the coating of gold nanoparticles with a peptide sequence derived from gliadin, the protein that triggers celiac disease.METHODS20 nm gold nanoparticles were first coated with NeutrAvidin. A long chain Polyethylene glycol (PEG) linker containing Maleimide at the Ω-end and Biotin group at the α-end was used to ensure peptide coating to the gold nanoparticles. The maleimide group with the thiol (-SH) side chain reacted with the cysteine amino acid in the peptide sequence and the biotinylated and PEGylated peptide was added to the NeutrAvidin coated gold nanoparticles. The peptide coated gold nanoparticles were then converted into a serological assay. We used the peptide functionalised gold nanoparticle-based assay on thirty patient serum samples in a blinded assessment and compared our results with the previously run serological and pathological tests on these patients.RESULTSA stable colloidal suspension of peptide coated gold nanoparticles was obtained without any aggregation. An absorbance peak shift as well as color change was caused by the aggregation of gold nanoparticles following the addition of anti-gliadin antibody to peptide coated nanoparticles at levels associated with celiac disease. The developed assay has been shown to detect anti-gliadin antibody not only in quantitatively spiked samples but also in a small-scale study on real non-hemolytic celiac disease patient’s samples.CONCLUSIONThe study demonstrates the potential of gold nanoparticle-peptide based approach to be adapted for developing a screening assay for celiac disease diagnosis. The assay could be a part of an exclusion based diagnostic strategy and prove particularly useful for testing high celiac disease risk populations.

show abstract

Electron density imaging of protein films on gold-particle surfaces with transmission electron microscopy

Cited by 26 publications

References 16 publications

Synthesis and characterization of jacalin-gold nanoparticles conjugates as specific markers for cancer cells

Synthesis and characterization of jacalin-gold nanoparticles conjugates as specific markers for cancer cells

Preparation of Colloidal Gold Immunochromatographic Strip for Detection of Paragonimiasis skrjabini

Novel screening test for celiac disease using peptide functionalised gold nanoparticles

Contact Info

Product

Resources

About