Electron Beam Irradiation to Styela Clava Derived Cellulose Membrane

Abstract

초록: 셀룰로오스는 탄소와 수소 성분으로 이루어진 탄수화물 복합체로서 β-글루코오스 성분으로 이루어진 식물 세 포벽의 주된 구성성분이다. 미더덕의 피부 각질에서 개발한 동물성 셀룰로오스 유도막에 1-2 MeV 에너지의 전자빔 을 조사하여 C-C 결합보다 C-O 결합이 보다 증가함을 확인하였고, 여러 관련된 결과를 바탕으로 전자빔 조사에 의 해 미세소관을 구성하는 셀룰로오스 미세원섬유들의 탈중합 과정을 일으킬 수 있었다. 셀룰로오스 합성복합체가 이 동함으로써 셀룰로오스 섬유소들을 균일하고 미세하게 변형시킴으로써 골유도재생술을 위한 얇은 μm 단위의 의료 용 유도막으로 활용이 가능할 것으로 사료된다.Abstract: Cellulose is the carbohydrate polymer composed of carbon, hydrogen and β-glucose, and the main composition of plant cell walls. The aim of this study is to evaluate the effect and potential of 1-2 MeV electron beam (E-beam) irradiation to the sea squirt derived cellulose membrane (CM) from Styela clava, called non-native tunicate. C-O bonding was increased than C-C bonding, and several related results showed depolymerization of cellulose microfibrils composed of microtubles. Cross-linking cellulose protein (CCP) was lost after E-beam irradiation, and so thin and delicate cellulose fibrils were detached each other by moving cellulose synthase complex. The potential of this cellulose polymer as a thin μm thickness medical membrane for the guided bone regeneration can be suggested.

Cellulose membrane as a biomaterial: from hydrolysis to depolymerization with electron beam

“…During our recent investigations of the effects of EBI on maxillofacial reconstructive polymer materials, we have tried to identify gross and elemental changes in E-beam irradiated CMs [ 9 – 11 ]. E-beam treatment involves accelerating a beam of electrons to near the speed of light and by utilizing an oscillating magnetic field, sweeping the electrons back and forth across the polymers.…”

“…We previously demonstrated that CM can be successfully used as a GBR membrane in combination with particulate bone grafting and that the peculiar characteristics of E-beam irradiated CM are also useful for space maintenance and biocompatibility [ 2 , 5 , 10 , 11 ]. Five surgical factors are required to achieve predictable results with GBR procedures: 1) use of an appropriate membrane, 2) achievement of primary soft tissue healing, 3) creation and maintenance of a membrane-protected space, 4) close adaptation and stabilization of the membrane to the surrounding bone, and 5) a sufficiently long healing period.…”

“…E-beam irradiated CM does not need a second surgical procedure for its removal due to its biocompatible longevity. The second surgery increases the risk of loss of regenerated bone on the flap reflection [ 1 , 11 ].…”

“…More recently, blended films have been prepared from native cotton linters and depolymerized cotton linters to alter regenerated CM properties such as water uptake, porosity, and tortuosity [ 38 ]. The changes in solute size hydrophobicity were achieved through E-beam irradiated regenerated CM [ 11 , 17 ]. The structure and properties of these membranes could be controlled and modified depending on the energy, type, and E-beam dose [ 39 ].…”

“…Cellulose binding domains (CBDs) are structurally and functionally independent; no catalytic modules nor essential elements are found in many cellulose or hemicelluloses degrading enzymes (Fig. 3 ) [ 11 , 40 , 41 ]. All CBDs have affinity for cellulose; they are divided into several families and do not have any hydrolytic activity [ 40 ].…”