Electrometric and Colorimetric Determination of Carbonic Anhydrase

Wilbur, Karl M.; Anderson, Norman G.

doi:10.1016/s0021-9258(18)51011-5

Cited by 972 publications

(119 citation statements)

References 10 publications

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“…Carbonic anhydrase activity was determined as reported by Wilbur and Anderson 54 . Leaves frozen samples (50 mg) were homogenized in 20 mM Tris HCl buffer (pH 8.3) containing 1 mM ethylenediamine tetraacetic acid (EDTA) and 10 mM 2-mercaptoethanol and centrifuged at 15,000 g for 5 min at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Unravelling homeostasis effects of phosphorus and zinc nutrition by leaf photochemistry and metabolic adjustment in cotton plants

Santos

Pongrac

Reis

et al. 2021

Sci Rep

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Phosphorus (P) and zinc (Zn) uptake and its physiological use in plants are interconnected and are tightly controlled. However, there is still conflicting information about the interactions of these two nutrients, thus a better understanding of nutritional homeostasis is needed. The objective of this work was to evaluate responses of photosynthesis parameters, P-Zn nutritional homeostasis and antioxidant metabolism to variation in the P × Zn supply of cotton (Gossypium hirsutum L.). Plants were grown in pots and watered with nutrient solution containing combinations of P and Zn supply. An excess of either P or Zn limited plant growth, reduced photosynthesis-related parameters, and antioxidant scavenging enzymes. Phosphorus uptake favoured photochemical dissipation of energy decreasing oxidative stress, notably on Zn-well-nourished plants. On the other hand, excessive P uptake reduces Zn-shoot concentration and decreasing carbonic anhydrase activity. Adequate Zn supply facilitated adaptation responses to P deficiency, upregulating acid phosphatase activity, whereas Zn and P excess were alleviated by increasing P and Zn supply, respectively. Collectively, the results showed that inter ionic effects of P and Zn uptake affected light use and CO2 assimilation rate on photosynthesis, activation of antioxidant metabolism, acid phosphatase and carbonic anhydrase activities, and plant growth-related responses to different extents.

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Section: Methodsmentioning

confidence: 99%

Unravelling homeostasis effects of phosphorus and zinc nutrition by leaf photochemistry and metabolic adjustment in cotton plants

Santos

Pongrac

Reis

et al. 2021

Sci Rep

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“…Measurement of CA activity CA activity was investigated in the blood of Covid-19 patients in Ankara Yildirim Beyazit University Medical Biology research laboratory with 29 non-Covid-19 patients, 29 acute Covid-19, and 47 post-Covid-19 patients. The enzyme activity was measured by the hydration of CO 2 according to the method of Wilbur and Anderson11 , which is based on the determination of the time required for the pH of solution decreasing from 10.0 to 7.4 due to the hydration of CO 2 . Assays were performed at least twice on each lysate, and the mean value was determined to the formula: EU¼(totc)/tc12 .…”

mentioning

confidence: 99%

Is carbonic anhydrase inhibition useful as a complementary therapy of Covid-19 infection?

Deniz

Uysal

Capasso

et al. 2021

Journal of Enzyme Inhibition and Medicinal Chemistry

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The ongoing Covid-19 is a contagious disease, and it is characterised by different symptoms such as fever, cough, and shortness of breath. Rising concerns about Covid-19 have severely affected the healthcare system in all countries as the Covid-19 outbreak has developed at a rapid rate all around the globe. Intriguing, a clinically used drug, acetazolamide (a specific inhibitor of carbonic anhydrase, CA, EC 4.2.1.1), is used to treat high-altitude pulmonary oedema (HAPE), showing a high degree of clinical similarities with the pulmonary disease caused by Covid-19. In this context, this preliminary study aims to provide insights into some factors affecting the Covid-19 patients, such as hypoxaemia, hypoxia as well as the blood CA activity. We hypothesise that patients with Covid-19 problems could show a dysregulated acidbase status influenced by CA activity. These preliminary results suggest that the use of CA inhibitors as a pharmacological treatment for Covid-19 may be beneficial.

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“…The protocol for the measurement of CA activity was based on the description by Wilbur and Anderson ( Wilbur and Anderson, 1948 ) with some modifications. CA activity was calculated using Wilbur–Anderson units (WAU) mg –1 protein.…”

Section: Methodsmentioning

confidence: 99%

Fungal Carbonyl Sulfide Hydrolase of <i>Trichoderma harzianum</i> Strain THIF08 and Its Relationship with Clade D β-Carbonic Anhydrases

et al. 2021

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Carbonyl sulfide (COS) is the most abundant and long-lived sulfur-containing gas in the atmosphere. Soil is the main sink of COS in the atmosphere and uptake is dominated by soil microorganisms; however, biochemical research has not yet been conducted on fungal COS degradation. COS hydrolase (COSase) was purified from Trichoderma harzianum strain THIF08, which degrades COS at concentrations higher than 10,000 parts per million by volume from atmospheric concentrations, and its gene cos (492 bp) was cloned. The recombinant protein purified from Escherichia coli expressing the cos gene converted COS to H 2 S. The deduced amino acid sequence of COSase (163 amino acids) was assigned to clade D in the phylogenetic tree of the β-carbonic anhydrase (β-CA) family, to which prokaryotic COSase and its structurally related enzymes belong. However, the COSase of strain THIF08 differed from the previously known prokaryotic COSase and its related enzymes due to its low reactivity to CO 2 and inability to hydrolyze CS 2 . Sequence comparisons of the active site amino acids of clade D β-CA family enzymes suggested that various Ascomycota, particularly Sordariomycetes and Eurotiomycetes, possess similar enzymes to the COSase of strain THIF08 with >80% identity. These fungal COSase were phylogenetically distant to prokaryotic clade D β-CA family enzymes. These results suggest that various ascomycetes containing COSase contribute to the uptake of COS by soil.

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Electrometric and Colorimetric Determination of Carbonic Anhydrase

Cited by 972 publications

References 10 publications

Unravelling homeostasis effects of phosphorus and zinc nutrition by leaf photochemistry and metabolic adjustment in cotton plants

Unravelling homeostasis effects of phosphorus and zinc nutrition by leaf photochemistry and metabolic adjustment in cotton plants

Is carbonic anhydrase inhibition useful as a complementary therapy of Covid-19 infection?

Fungal Carbonyl Sulfide Hydrolase of <i>Trichoderma harzianum</i> Strain THIF08 and Its Relationship with Clade D β-Carbonic Anhydrases

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