Electromagnetic Stimulation of Buds of Castanea Sativa Mill. In Tissue Culture

Abstract

Buds of chestnut (Castanea sativa, Mill.) were grown on modified Heller's solid material medium with the addition of 0.1 mg/mL 6-benzylaminopurine (BAP). They were divided monthly and used for experiments with homogeneous sinusoidal magnetic fields of 5.9, 3.2, and 1.2 mT for 1 h/day, 6 days a week, for 28 weeks.The results are season-dependent; bud growth was significantly enhanced over controls in early summer (50% at 5.0 mT, 64% at 3.2 mT, and 62% at 1.2 mT). In late summer and fall, growth was inhibited slightly. In early winter, it was stimulated again. We also measured significant effects 1 month after cessation of stimulation.

Diamagnetic Susceptibility and Root Growth Responses to Magnetic Fields in Lens culinaris, Glycine soja, and Triticum aestivum

“…The diversity of responses among the studied species and seasons is in agreement with the existence of several reports on the difficult repeatability of experiments with magnetic fields (Davis and Rawls, 1987;Ruzic et al, 1992). It seems that biological systems during phases of intensive growth, such as those tested here and in suboptimal environmental conditions such as under drought stress (Ruzic et al, 1998a) or under acid environments (Ruzic et al, 1998b), are prone to exhibit greater sensitivity to low fields (Ruzic et al, 1998a,b).…”

“…It seems that biological systems during phases of intensive growth, such as those tested here and in suboptimal environmental conditions such as under drought stress (Ruzic et al, 1998a) or under acid environments (Ruzic et al, 1998b), are prone to exhibit greater sensitivity to low fields (Ruzic et al, 1998a,b). The effect seems to depend on the physiological state of the plant tissue (Ruzic et al, 1992). Therefore, the response would depend not only on the magnetic induction and its gradient (Wittekind et al, 1990), but also on the physiological state of the experimental organism, as proposed by Mcleod et al (1992).…”

Effects of electrically transferred molecular information on the germination of garden cress

“…From many studies it is known that biological effects vary according to the season of the year. For example, a weak magnetic field may produce different outcomes in different times of the year [39,40]. It is similar with some physiologically effective chemicals, like the effects of plant hormones, that vary in relation to the season of application (spring or autumn) [41].…”