Electromagnetic fields increase in vitro and in vivo angiogenesis through endothelial release of FGF‐2

Tepper, Oren M.; Callaghan, Matthew J.; Chang, Edward I.; Galiano, Robert D.; Bhatt, Kirit A.; Baharestani, Samuel; Gan, Jean; Simon, Bruce J.; Hopper, Richard A.; Levine, Jamie P.; Gurtner, Geoffrey C.

doi:10.1096/fj.03-0847fje

Cited by 174 publications

(152 citation statements)

References 21 publications

(19 reference statements)

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“…Most investigations on the mechanism of action of IC technology have been performed with the PEMF signal which is FDA approved for fracture healing but not spinal fusion [1,4,8,11,29,43,44,51,64]. There is only one study using the PEMF signal that is clinically approved for spinal fusions [61] and a few studies with CMF [11,20,21,58].…”

Section: Inductive Couplingmentioning

confidence: 99%

Electrical stimulation therapies for spinal fusions: current concepts

Gan

Glazer

2006

Eur Spine J

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Electrical stimulation therapies have been used for more than 30 years to enhance spinal fusions. Although their positive effects on spinal fusions have been widely reported, the mechanisms of action of the technologies were only recently identified. Three types of technologies are available clinically: direct current, capacitive coupling, and inductive coupling. The latter is the basis of pulsed electromagnetic fields and combined magnetic fields. This review summarizes the current concepts on the mechanisms of action, animal and clinical studies, and cost justification for the use of electrical stimulation for spinal fusions. Scientific studies support the validity of electrical stimulation treatments. The mechanisms of action of each of the three electrical stimulation therapies are different. New data demonstrates that the upregulation of several growth factors may be responsible for the clinical success seen with the use of such technologies.

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Section: Inductive Couplingmentioning

confidence: 99%

Electrical stimulation therapies for spinal fusions: current concepts

Gan

Glazer

2006

Eur Spine J

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“…However, SMF did not induce any significant changes in the VEGF-A-increased number of bifurcations in any part of the wells [25]. Additionally it is reported that Pulsed electromagnetic fields (PEMF) had ability to increase in vitro and in vivo angiogenesis through endothelial release of FGF-2 and inducing paracrine and autocrine changes in the surrounding tissues [26]. In 2001 Williams reported that exposure to therapeutic electromagnetic fields (10 minutes per day with 0.…”

Section: Discussionmentioning

confidence: 99%

Reduction VEGF-A expression induced by saffron aqua extract with low frequency electromagnetic field in MCF-7 cell line

Mousavi¹,

Baharara²

2014

The Journal of Genes, Microbes and Immunity

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Background: Studies both invivo and invitro indicated anticancer and antitumor properties of saffron and its component against different human cancer cells which is wildly used as a drug to promote health and fight diseases from ancient time. Angiogenesis, formation of new blood vessels, is regulated by the balances of many stimulating and inhibiting factors. While physiological angiogenesis is under strike control, disruption of this control causes proliferation of a network of blood vessels penetrating into cancerous growth. One of the main factors which are involved in the process of angiogenesis in both physiologic and pathologic condition is VEGF-A. On the other hand, bio electromagnetic fields have been shown to be clinically beneficial in the mT amplitude range. The aim of this study was finding out if synergic effects of saffron aqueous extract and low-frequency electromagnetic field could decrease angiogenesis via reduction of VEGF-A gene expression.Materials and methods: In this experimental study, saffron extract obtained using lyophilized by freeze dryer. MCF-7 cells were grown in RPMI 1640 medium supplemented with %10 FBS and incubated at 37˚C with %5 CO2. After 24h cells were treated by saffron aqueous extract at concentration of 100, 200, 400 and 800 µg/ml. 48 h after treatment all flasks exposed with electromagnetic field then total RNA extracted, cDNA synthesized using the sequence of target gene. Finally synthesized products analyzed by Real Time PCR to determine expression level of VEGF-A. The quantitative data analyzed by SPSS software. P value ˂0.05 was considered statistically significant.Results: Data analysis indicate inhibitory effect of saffron extract in concentration 100, 200, 400 and 800µg/ml on VEGF-A gene expression in MCF-7 cell line in compare with control. Most reduction in gene expression can be seen in the highest concentration of saffron extract (800 µg/ml) with 17% reduction on gene expression (P˂0.001), while there is no difference between 100 and 200 µg/ml (P˃0.05) but comparing to control group it is also fall noticeable with 11% and 12% respectively (P˂0.05). Synergic use of low frequency electromagnetic field (50Hz, 0.004 T) and saffron aqua extract shows reduction in concentration 100 to 800 µg/ml with 17%, 13%, 15% and 19% respectively and was significant at concentration of 800µg/ml (P˂0.001).Conclusion: Results shows decrease in the expression of VEGF-A in the treated samples with saffron extract and low frequency electromagnetic field which may introduce promising treatment against malignant and tumor metastasis, although more researches needs to figure out its accurate mechanism.

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“…One study reported acceleration of angiogenesis within 1 week of commencing electromagnetic therapy [27]. Another study reported ischemia in the bone occurs within 5 to 6 days after corticosteroid administration [10].…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, prevention of bone ischemia might also prevent steroid-induced ON. We considered pulsed electromagnetic field stimulation could be a preventive therapy because it promotes angiogenesis [11,19,27] and dilates blood vessels [23]. We speculated it might also influence the enlargement of intramedullary fat cells since that is reportedly a potential mechanism in bone necrosis [14,15,28].…”

Section: Introductionmentioning

confidence: 99%

Electromagnetic Fields

Ishida

Fujioka

Takahashi

et al. 2008

Clinical Orthopaedics &Amp; Related Research

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Establishing a means to prevent osteonecrosis after corticosteroid administration is an important theme. We asked whether pulsed electromagnetic field stimulation, a noninvasive treatment, could prevent osteonecrosis. Ninety rabbits were divided into four treatment groups: (1) exposure of 10 hours per day to electromagnetic stimulation for 1 week, followed by injection of methylprednisolone (20 mg/kg), and exposure of 10 hours per day to electromagnetism for a further 4 weeks (n = 40); (2) methylprednisolone injection only (n = 40); (3) no treatment (n = 5); and (4) exposure of 10 hours per day to electromagnetism for 5 weeks (n = 5). After 5 weeks, we harvested and histologically examined femurs bilaterally. The frequency of osteonecrosis was lower in the steroidelectromagnetism group (15/40) than in the steroid-only group (26/40). No necrotic lesions were found in the two control groups. We observed no clear effects of electromagnetism on the number, location, extent, and repair of necrotic lesions and intramedullary fat cell size in affected rabbits. Pulsed electromagnetic field stimulation reportedly augments angiogenesis factors and dilates blood vessels; these effects may lower the frequency of osteonecrosis. Exposure to pulsed electromagnetic field stimulation before corticosteroid administration could be an effective means to reduce the risk of osteonecrosis.

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Electromagnetic fields increase in vitro and in vivo angiogenesis through endothelial release of FGF‐2

Cited by 174 publications

References 21 publications

Electrical stimulation therapies for spinal fusions: current concepts

Electrical stimulation therapies for spinal fusions: current concepts

Reduction VEGF-A expression induced by saffron aqua extract with low frequency electromagnetic field in MCF-7 cell line

Electromagnetic Fields

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