“…These state-of-art imaging systems are, widefield fluorescence microscopy, confocal microscopy and super-resolution 4pi microscopy. The system PSF h sys for a widefield microscope is, h(x, y, 28,29,27,30 In a confocal microscope, there is an additional pinhole P at the detector arm to eliminate out-of-focus light from the fluorescent sample. The system PSF for a confocal system can be expressed as h sys = h ill × (h det ⊗ P).…”