“…Perchlorate is not inhibiting the enzyme at concentrations up to 100 mM, data not shown, as for many other CAs investigated earlier), following the initial rates of the CA-catalyzed CO 2 hydration reaction for a period of 10-100 s. 29 The CO 2 concentrations ranged from 1.7 to 17 mM for the determination of the kinetic parameters and inhibition constants. Lineweaver-Burk plots were used for determining the kinetic parameters.…”