Electrokinetically controlled real-time polymerase chain reaction in microchannel using Joule heating effect

Hu, Guoqing; Xiang, Qing; Fu, Rachel; Xu, Bo; Venditti, Roberto; Li, Dongqing

doi:10.1016/j.aca.2005.10.021

Cited by 62 publications

(71 citation statements)

References 34 publications

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“…The heater heats its internal and external area in 1 ms by Joule heating generated by a thermal source. 55 Thus far, there have been only a few reports on heaters fabricated with conducting PDMS composites, which probably reflect difficulties entailed in electrode fabrication and thermal field distribution in PDMS matrices as well as inertial thermal conductivity ͑about 0.15 W / m K͒. However, doping conductive particles such as silver and carbon cannot only increase the electrical conductivity but also thermal conductivity.…”

Section: E Microfluidic Heatermentioning

confidence: 99%

Polydimethylsiloxane-based conducting composites and their applications in microfluidic chip fabrication

Gong

Wen

2009

Biomicrofluidics

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This paper reviews the design and fabrication of polydimethylsiloxane ͑PDMS͒-based conducting composites and their applications in microfluidic chip fabrication. Owing to their good electrical conductivity and rubberlike elastic characteristics, these composites can be used variously in soft-touch electronic packaging, planar and three-dimensional electronic circuits, and in-chip electrodes. Several microfluidic components fabricated with PDMS-based composites have been introduced, including a microfluidic mixer, a microheater, a micropump, a microdroplet controller, as well as an all-in-one microfluidic chip.

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Section: E Microfluidic Heatermentioning

confidence: 99%

Polydimethylsiloxane-based conducting composites and their applications in microfluidic chip fabrication

Gong

Wen

2009

Biomicrofluidics

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“…24,[55][56][57][58][59] An electrokinetically controlled DNA hybridization microfluidic chip has been demonstrated and can perform all processes from sample dispensing to hybridization detection within 5 min. 24 The chip was composed of a PDMS upper substrate and a lower glass substrate that served as a substrate for the hybridization array, as shown in Figure 4.…”

Section: Nonmechanical Approachmentioning

confidence: 99%

“…Moreover, the microfluidic chip for real-time PCR has been reported using a Joule heating effect. 55 The chip was fabricated by PDMS and glass substrates. Joule heating was generated in an electrokinetically driven microfluidic chip by the current flow through the buffer solution.…”

Section: Nonmechanical Approachmentioning

confidence: 99%

Microfluidic Systems for Diagnostic Applications: A Review

Lei

2012

SLAS Technology

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Because of intensive developments in recent years, the microfluidic system has become a powerful tool for biological analysis. Entire analytic protocols including sample pretreatment, sample/reagent manipulation, separation, reaction, and detection can be integrated into a single chip platform. A lot of demonstrations on the diagnostic applications related to genes, proteins, and cells have been reported because of their advantages associated with miniaturization, automation, sensitivity, and specificity. The aim of this article is to review recent developments in microfluidic systems for diagnostic applications. Based on the categories of various fluid-manipulating mechanisms and biological detection approaches, in-depth discussion of the microfluidic-based diagnostic systems is provided. Moreover, a brief discussion on materials and manufacturing techniques will be included. The current excellent integration of microfluidic systems and diagnostic applications suggests a solid foundation for the development of practical point-of-care devices.

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“…82,84 Several methods have been used to achieve temperature control of the reaction mixture in a microfluidic chip. These have included microfabricated heater elements, 85,86 Joule heating, 87 noncontact heating, 88 and Peltier elements, 89 among others.…”

Section: Pcr-based Assaysmentioning

confidence: 99%

Droplet-Based Microfluidics: Enabling Impact on Drug Discovery

et al. 2014

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Over the past two decades, the application of microengineered systems in the chemical and biological sciences has transformed the way in which high-throughput experimentation is performed. The ability to fabricate complex microfluidic architectures has allowed scientists to create new experimental formats for processing ultra-small analytical volumes in short periods and with high efficiency. The development of such microfluidic systems has been driven by a range of fundamental features that accompany miniaturization. These include the ability to handle small sample volumes, ultra-low fabrication costs, reduced analysis times, enhanced operational flexibility, facile automation, and the ability to integrate functional components within complex analytical schemes. Herein we discuss the impact of microfluidics in the area of high-throughput screening and drug discovery and highlight some of the most pertinent studies in the recent literature.

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Electrokinetically controlled real-time polymerase chain reaction in microchannel using Joule heating effect

Cited by 62 publications

References 34 publications

Polydimethylsiloxane-based conducting composites and their applications in microfluidic chip fabrication

Polydimethylsiloxane-based conducting composites and their applications in microfluidic chip fabrication

Microfluidic Systems for Diagnostic Applications: A Review

Droplet-Based Microfluidics: Enabling Impact on Drug Discovery

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