Electrokinetic Study of Bovine Serum Albumin Adsorption on Previously Formed PAH/PSS Multilayer

Đapić, Irena; Kovačević, Davor

doi:10.5562/cca1822

Cited by 5 publications

(3 citation statements)

References 24 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of fetal bovine serum in the experiment environments, which partly reflects an in vivo environment, introduces dozens of proteins which interact with particle surfaces and contribute to further particle-cell interactions [ 25 ]. Due to the high concentration of negatively charged albumin in the cell growth media, adsorption on the positively charged PAH-coated particle surface is a possible scenario [ 37 ]. Albumin -oated particles are known as poorly-phagocyted variants of modified particles, due to the hydrophilic nature of their surfaces [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Microparticles of Lamivudine—Poly-ε-Caprolactone Conjugate for Drug Delivery via Internalization by Macrophages

et al. 2019

View full text Add to dashboard Cite

The past decade may be considered as revolutionary in the research field focused on the physiological function of macrophages. Unknown subtypes of these cells involved in pathological mechanisms were described recently, and they are considered as potential drug delivery targets. The innate ability to internalize foreign bodies exhibited by macrophages can be employed as a therapeutic strategy. The efficiency of this uptake depends on the size, shape and surface physiochemical properties of the phagocyted objects. Here, we propose a method of preparation and preliminary evaluation of drug-polymer conjugate-based microspheres for macrophage targeted drug delivery. The aim of the study was to identify crucial uptake-enhancing parameters for solid, surface modified particles. A model drug molecule—lamivudine—was conjugated with poly-ε-caprolactone via ring opening polymerization. The conjugate was utilized in a solvent evaporation method technique to form solid particles. Interactions between particles and a model rat alveolar cell line were evaluated by flow cytometry. The polymerization product was characterized by a molecular weight of 3.8 kDa. The surface of the obtained solid drug-loaded cores of a hydrodynamic diameter equal to 2.4 µm was modified with biocompatible polyelectrolytes via a layer-by-layer assembly method. Differences in the internalization efficiency of four particle batches by the model RAW 264.7 cell line suggest that particle diameter and surface hydrophobicity are the most influential parameters in terms of phagocytic uptake.

show abstract

Section: Discussionmentioning

confidence: 99%

Microparticles of Lamivudine—Poly-ε-Caprolactone Conjugate for Drug Delivery via Internalization by Macrophages

et al. 2019

View full text Add to dashboard Cite

show abstract

“…1 that the electrophoretic mobility of all macroions is positive for a broad pH range and ionic strength up to 0.15 M. However, at pH larger than 8, the electrophoretic mobility of PAH and PLL abruptly decreases and vanishes at pH equal to 10 and 9.5, respectively. These pH values can be identified as the isoelectric point (IEP) of these macroions [51][52][53]. In the case of PDDA its electrophoretic mobility remains positive for pH up to 11, hence the molecules exhibit no IEP.…”

Section: Bulk Macroion Characteristicsmentioning

confidence: 99%

Modyfikacja Substratów Stałych Przez Kontrolowaną Adsorpcję Makrojonów

Morga¹

2021

WiadChem

View full text Add to dashboard Cite

Abbreviations and symbols 1. Introduction 2. Experimental methods 3. Bulk macroion characteristics 4. Macroion adsorption -monolayer characteristics 5. Macroion layer stability 6. Conclusions Dr inż. Maria Morga -doktor nauk chemicznych od 2014 roku. Adiunkt w Instytucie Katalizy i Fizykochemii Powierzchni im. Jerzego Habera Polskiej Akademii Nauk. Absolwentka jednolitych studiów magisterskich Politechniki Krakowskiej, wydziału Inżynierii i Technologii Chemicznej, absolwentka studiów podyplomowych wydziału Inżynierii Materiałowej i Ceramiki (Biomateriały -Materiały dla Medycyny).Kierownik projektu NCN Sonata o tematyce z zakresu wytwarzania monowarstw i multiwarstw nanocząstek i makrojonów: polielektrolitów, polipeptydów na powierzchniach granicznych ciało stałe-ciecz, określania kinetyki adsorpcji makrojonów na powierzchniach stałych oraz badaniu stabilności monowarstw makrojonów w zależności od warunków środowiska. Współautorka 35 publikacji oraz 1 patentu. Wykonawca 8 projektów naukowych NCN i Funduszy Strukturalnych UE. Szerokie doświadczenie w prowadzeniu badań z zastosowaniem technik mikroelektroforezy kapilarnej oraz metody DLS do charakterystyki makrojonów w objętości oraz w prowadzeniu badań nad charakterystyką multiwarstw nanocząstek na powierzchni ciał stałych i określaniu właściwości fizykochemicznych powierzchni substratów w tym właściwości elektrokinetycznych powierzchni oraz monowarstw nanocząstek i makrojonów z użyciem metody potencjału przepływu.

show abstract

“…PEMs and protein-PEMs) play a very important role in surface modification processes, it was intriguing to check if other experimental methods would confirm the results and conclusions obtained by stagnation point optical reflectometry. Therefore, electrokinetic measurements were also applied for the investigation of PAH/PSS multilayer formation as a function of pH, with PAH being a terminal layer [35]. The instrument uses electrophoretic light scattering and the Laser Doppler Velocimetry method for determination of particle velocity, and from this the electrokinetic zeta potential can be determined.…”

Section: Polyelectrolyte Multilayersmentioning

confidence: 99%