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Cited by 28 publications
References 165 publications
The article contains sections titled: 1. Introduction 2. Cell Disruption 3. Solid–Liquid Separation 3.1. Conventional Filtration 3.2. Membrane Filtration 3.2.1. Promotion of Turbulence 3.2.2. Application of Electric Field 3.3. Centrifugation 3.4. Coagulation and Flocculation 4. Product Isolation 4.1. Extraction 4.2. Adsorption Including Ion Exchange 4.2.1. Affinity Adsorption 4.2.2. Ion‐Exchange Adsorption 4.2.3. Quantitative Analysis 4.2.4. Elution 4.3. Precipitation 4.3.1. Salting Out 4.3.2. pH Adjustment 4.3.3. Organic Solvents 4.3.4. Temperature 4.3.5. Polymers and Polyelectrolytes 4.3.6. Affinity Precipitation 4.4. Solubilization and Refolding 4.5. Ultrafiltration 5. Purification 5.1. Chromatography 5.1.1. Gel Filtration Chromatography 5.1.2. Affinity Chromatography 5.1.3. Reverse Phase and Hydrophobic Interaction Chromatography 5.1.4. Quantitative Aspects 5.2. Electrical Separations 5.2.1. Electrophoresis 5.2.2. Isoelectric Focusing and Isotachophoresis 5.2.3. Electrodialysis 5.2.4. Dielectrophoresis 5.3. Magnetic Separations 5.4. Membrane Chromatography 6. Polishing 6.1. Crystallization 6.2. Drying and Freeze Drying 6.3. Buffer Exchange 6.4. Virus and Endotoxin Removal 6.5. Removal of Aggregates 7. New Bioseparation Strategies
The article contains sections titled: 1. Introduction 2. Basic Principles 3. Electrophoretic Matrices 3.1. Cellulose Acetate 3.2. Agarose Gels 3.3. Polyacrylamide Gels 4. Discontinuous Electrophoresis 5. Isoelectric Focusing 6. Sodium Dodecyl Sulfate Electrophoresis 7. Porosity Gradient Gels 8. Two‐Dimensional Maps (Proteome Analysis) 9. Isotachophoresis 10. Immunoelectrophoresis 10.1. Rocket Immunoelectrophoresis 10.2. Crossed Immunoelectrophoresis 10.3. Tandem Crossed Immunoelectrophoresis 10.4. Intermediate Gel Crossed Immunoelectrophoresis 10.5. Fused‐Rocket Crossed Immunoelectrophoresis 11. Staining Techniques and Blotting 12. Immobilized pH Gradients 13. Capillary Zone Electrophoresis 14. Preparative Electrophoresis 14.1. Preparative Isoelectric Focusing in Granulated Gel Layers 14.2. Continuous‐Flow, Recycling Isoelectric Focusing 14.3. The Rotofor 14.4. Recycling Free‐Flow Focusing (RF3) 14.5. Multicompartment Electrolyzers with Isoelectric Membranes
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