Electrokinetic Properties of Aqueous Suspensions of Rodlike fd Virus Particles in the Gas- and Liquidlike Phase

Hoß, U.; Batzill, Stefan; Deggelmann, M.; Gräf, Christian; Hagenbuechle, Martin; Johner, Christian; Krämer, H.; Martin, C.; Overbeck, Ekkehard; Weber, R.

doi:10.1021/ma00090a044

Cited by 8 publications

(16 citation statements)

References 10 publications

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“…[45] It is easily checked that if the two particles are identical and ka r ϱ , then there is no interaction between the particles; i.e., both have the velocity they would have in Figure 2 shows the contributions of the various reflection renormalization of this term has a significant effect on the average mobility, as will be seen in the next section.…”

Section: Mobility Of Two Spheresmentioning

confidence: 86%

Electrophoretic Mobility of a Semi-dilute Suspension of Spherical Particles with Thick Double Layers and Low Zeta Potentials

Ennis

White

1997

Journal of Colloid and Interface Science

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Section: Mobility Of Two Spheresmentioning

confidence: 86%

Electrophoretic Mobility of a Semi-dilute Suspension of Spherical Particles with Thick Double Layers and Low Zeta Potentials

Ennis

White

1997

Journal of Colloid and Interface Science

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“…Experimentally, most electrophoresis measurements were done using capillairy zone electrophoresis 13 or electrophoretic light scattering. 25 , 26 Using these techniques, only orientationally averaged electrophoretic mobilities of the rodlike particles can be obtained. On the other hand, using optical microscopy, one can measure the electrophoretic mobility of single rodlike particles and its dependence on the orientation with respect to the applied electric field.…”

Section: Introductionmentioning

confidence: 99%

Microelectrophoresis of Silica Rods Using Confocal Microscopy

et al. 2017

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The electrophoretic mobility and the zeta potential (ζ) of fluorescently labeled colloidal silica rods, with an aspect ratio of 3.8 and 6.1, were determined with microelectrophoresis measurements using confocal microscopy. In the case where the colloidal particles all move at the same speed parallel to the direction of the electric field, we record a xyz-stack over the whole depth of the capillary. This method is faster and more robust compared to taking xyt-series at different depths inside the capillary to obtain the parabolic flow profile, as was done in previous work from our group. In some cases, rodlike particles do not move all at the same speed in the electric field, but exhibit a velocity that depends on the angle between the long axis of the rod and the electric field. We measured the orientation-dependent velocity of individual silica rods during electrophoresis as a function of κa, where κ–1 is the double layer thickness and a is the radius of the rod associated with the diameter. Thus, we determined the anisotropic electrophoretic mobility of the silica rods with different sized double layers. The size of the double layer was tuned by suspending silica rods in different solvents at different electrolyte concentrations. We compared these results with theoretical predictions. We show that even at already relatively high κa when the Smoluchowski limiting law is assumed to be valid (κa > 10), an orientation dependent velocity was measured. Furthermore, we observed that at decreasing values of κa the anisotropy in the electrophoretic mobility of the rods increases. However, in low polar solvents with κa < 1, this trend was reversed: the anisotropy in the electrophoretic mobility of the rods decreased. We argue that this decrease is due to end effects, which was already predicted theoretically. When end effects are not taken into account, this will lead to strong underestimation of the experimentally determined zeta potential.

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“…The first relevant theory 16 and systematic experimental investigation 17 of polyelectrolyte electrophoresis appeared in the 1930s. Subsequent investigations of free solution electrophoresis have focused mostly on biopolymers, 3,7,8,13,[18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] but a significant number of synthetic polymers have also been examined. 1,28,[35][36][37][38][39][40][41][42][43][44][45][46][47] Figure 1, compiling data from eight independent sources, 19,20,[23][24][25]27,28,34 shows µ 0 as a function of I for high molecular weight duplex DNA in aqueous NaCl; this system has been studied most often.…”

Section: Introductionmentioning

confidence: 99%

“…Even more confusing, a maximum of µ 0 with I has been reported in a few cases. 33,34,37,40,42,43 Many literature data have been obtained in complex buffer or electrolyte systems which contain components that bind to the migrating polyelectrolyte. 13,36 Theory A. Overview of Physical Principles.…”

Section: Introductionmentioning

confidence: 99%

“…The first relevant theory and systematic experimental investigation of polyelectrolyte electrophoresis appeared in the 1930s. Subsequent investigations of free solution electrophoresis have focused mostly on biopolymers, ,,,,− but a significant number of synthetic polymers have also been examined. ,,− Figure , compiling data from eight independent sources, ,,− ,,, shows μ 0 as a function of I for high molecular weight duplex DNA in aqueous NaCl; this system has been studied most often. The large disparities in the data sets of Figure plainly reveal why theoretical models for μ 0 have been so difficult to evaluate.…”

Section: Introductionmentioning

confidence: 99%

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Capillary Electrophoresis Measurements of the Free Solution Mobility for Several Model Polyelectrolyte Systems

1999

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The free solution electrophoretic mobilities of poly(styrenesulfonate), ss-DNA, and duplex DNA are measured by capillary electrophoresis across a range of ionic strengths and, for poly(styrenesulfonate) and ss-DNA, across a range of chain lengths. The data are then compared with mobilities reported in the literature and predicted by theory. For ionic strengths below 0.1 M, the capillary method is more accurate and rapid than previous techniques; it also provides a distribution of mobility values for polyelectrolyte mixtures. A maximum of the free solution mobility with respect to chain length is discovered in the oligomer range for both poly(styrenesulfonate) and ss-DNA; lowering ionic strength accentuates this unexplained phenomenon. In the large chain limit, where the mobility is independent of chain length, the ionic strength dependences of mobility for all three polymers are remarkably similar. These dependences can only be explained by models that incorporate nonlinear electrostatic effects into the description of the counterion cloud. The Manning model (with relaxation correction) best approximates the dependence of mobility on ionic strength.

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Electrokinetic Properties of Aqueous Suspensions of Rodlike fd Virus Particles in the Gas- and Liquidlike Phase

Cited by 8 publications

References 10 publications

Electrophoretic Mobility of a Semi-dilute Suspension of Spherical Particles with Thick Double Layers and Low Zeta Potentials

Electrophoretic Mobility of a Semi-dilute Suspension of Spherical Particles with Thick Double Layers and Low Zeta Potentials

Microelectrophoresis of Silica Rods Using Confocal Microscopy

Capillary Electrophoresis Measurements of the Free Solution Mobility for Several Model Polyelectrolyte Systems

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