Electrokinetic Phenomena of Acetone Through Sintered Glass G-4 Supported Lipid Membranes

Studies on Electrokinetic Effects Across Supported Mixed Lipid Membranes

“…(a) Purification of Chemicals: é Phospholipids (Bovine Brain Koch Ltd., England) acetone, methanol and Chloroform (A. R., B. D. H.) were purified in the same way described earlier [1,2]. The pure lipids were stored in a vacuum desiccator in order to prevent decomposition.…”

“…An electroosmotic cell with a pyrex sintered G 4 glass disc fused in the middle [1] was washed several times successively with hot 1:1 HNO 3 and water. It was then dried for 24 hours for the deposition of the lipid layer.…”

“…In previous studies, single lipid membranes, supported by pyrex sintered G 4 glass discs, have been considered and electrokinetic effects of water, acetone and their mixtures of various compositions have been measured [1][2][3], Water and acetone are present in most bookes of animals and the plasma membrane, surrounding a biological cell contains a mixture of lipids, proteins, enzymes etc. In order to have some basic information of the transfer of liquids across cellular membranes, artificial membranes, having two lipids together, were prepared.…”

Electrokinetic Effects off Aqueous Acetone Transmitting Supported Lipid Membranes

“…Lipid membranes of cephalin and phosphatidyl inositol were prepared in the same way described earlier [9,10]. The hydraulic permeability, electroosmosis, streaming potential and current were measured in an electroosmotic cell by the same method which already has been described in [9,10].…”

“…The hydraulic permeability, electroosmosis, streaming potential and current were measured in an electroosmotic cell by the same method which already has been described in [9,10]. All necessary precautions were taken and sources of error avoided, [11].…”