Electrokinetic-enhanced bioremediation of tetrachloroethylene

Chang, Chih-Hao; Yang, Hongta; Chen, Sheng-Kung; Hung, Jui-Min; Lu, Chun‐Ping; Liu, Min-Hsin

doi:10.1016/j.ibiod.2018.04.013

Cited by 9 publications

(4 citation statements)

References 39 publications

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“…were used as microbial sources for the anaerobic simulated aquifer. These anaerobic microorganisms were acclimated within a chemostat and isolated in a previous study (Chang et al, 2018).…”

Section: Simulated Aquifer Testsmentioning

confidence: 99%

Cometabolic biodegradation of chlorinated ethenes with methanotrophs in anaerobic/aerobic simulated aquifer

Chen¹,

Chin²,

Yang³

et al. 2021

JEB

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Aim: This study explores anaerobic/aerobic biodegradation efficiencies of aerobic cometabolism with methanotrophs when contaminants trichloroethylene (TCE) and cis-1,2-dichloroethylene (cDCE) are present individually or in tandem. Methodology: Batch tests and an anaerobic/aerobic column system were used to simulate saturated, contaminated aquifers. A brown glass bottle with an effective volume of 44 m l-1 was prepared for the batch test. An integrated one-dimensional sequential anaerobic/aerobic column system was used to simulate the accumulative intermediates such as TCE, cDCE and VC caused by incomplete degradation of PCE during the upgradient anaerobic stage in the saturated aquifer. In the downgradient aquifer, aerobic cometabolism was employed to degrade the intermediates. Methanotrophs in the aerobic aquifer were inoculated to degrade the by-products of incomplete degradation of PCE by aerobic cometabolism. Results: In the batch test, biodegradation of TCE was significantly inhibited by cDCE. However, biodegradation of cDCE was not significantly inhibited by TCE. In the simulated aquifer test, aerobic cometabolism completely degraded intermediates (TCE, cDCE, and VC) produced by incomplete anaerobic degradation of tetrachloroethylene (PCE). The results showed that methane, a by-product of anaerobic reductive dechorination of PCE, was used as a primary substrate for aerobic degradation, at a utilization rate of almost 100%. Interpretation: Biodegradation of TCE was significantly inhibited by cDCE. Bioremediation should have sufficient oxygen and methane at aerobic stage to ensure that chlorinated ethenes fully mineralize.

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“…were used as microbial sources for the anaerobic simulated aquifer. These anaerobic microorganisms were acclimated within a chemostat and isolated in a previous study (Chang et al, 2018).…”

Section: Simulated Aquifer Testsmentioning

confidence: 99%

Cometabolic biodegradation of chlorinated ethenes with methanotrophs in anaerobic/aerobic simulated aquifer

Chen¹,

Chin²,

Yang³

et al. 2021

JEB

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“…were used as microbial sources for the anaerobic sand column. These microorganisms were acclimated and isolated in a previous study (Chang et al 2018).…”

Section: Anaerobic Columnmentioning

confidence: 99%

“…The batch reactor is a brown glass bottle with an effective volume of 44 mL, wherein 2.5 mL of inorganic nutrient was added, and 15 mL of either the aerobic mixed bacteria (isolated in previous study by Chang et al 2018) or methane oxidizing bacteria (Table 3) was added. After the addition of the inorganic nutrient and bacterial culture solution, the bottle was sealed with a switch cover and covered with a Teflon gasket.…”

Section: Batch Testsmentioning

confidence: 99%

“…Many studies indicate that the combination of anaerobic and aerobic technologies can overcome the shortcomings of reductive dechlorination and completely mineralize CEs (Lee et al 1999;Tiehm and Schmidt 2011;Frascari et al 2013;Yoshikawa et al 2017). Another study pointed out that the electrolysis of water to stimulate anaerobic reductive dechlorination and aerobic oxidative metabolism is also efficient at removing CEs (Chang et al 2018). In most remediation sites, an additional fermentation substrate is often added during the anaerobic reductive dechlorination process (Aulenta et al 2007), which results in large amounts of methane generation.…”

Section: Introductionmentioning

confidence: 99%

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Complete degradation of chlorinated ethenes and its intermediates through sequential anaerobic/aerobic biodegradation in simulated groundwater columns (complete degradation of chlorinated ethenes)

Chen

Yang

Huang

et al. 2020

Int. J. Environ. Sci. Technol.

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This study evaluated the effectiveness of sequential anaerobic/aerobic biodegradation of tetrachloroethene (PCE) and its intermediates, cis-1,2-dichloroethene (cDCE) and vinyl chloride (VC). Two sand columns were operated in series. The first column simulated the up-gradient side of a groundwater system, was operated under anaerobic conditions, and was continuously fed the target contaminant, PCE (42 µM). The second column simulated the down-gradient side of the groundwater system and was operated under aerobic conditions, using low concentrations of hydrogen peroxide as the dissolved oxygen source. After 15 days of operation, cDCE was detected at the end of the first, anaerobic column, at concentrations of 7.02-15.57 μM. After 36 days of operation, VC (7.32 μM) was also detected at the end of the first column. cDCE and VC then migrated into the second, aerobic column. Results showed that cDCE and VC were almost completely aerobically biodegraded in the second column, with removal efficiencies of up to 97% and 95%, respectively. This study also used batch experiments to compare cDCE removal efficiencies between aerobic metabolism using cDCE as the only substrate, and aerobic cometabolism using methane and cDCE as primary and secondary substrates. Results showed that aerobic cometabolism of cDCE was inhibited at cDCE concentrations greater than 50 mg/L. This inhibition effect was not obvious under aerobic metabolism using cDCE as the only substrate. Results of a Michaelis-Menten/Monod kinetics analysis showed that when cDCE concentrations were greater than 20 mg/L, cDCE could be biodegraded more effectively under aerobic metabolism than under aerobic cometabolism.

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Modern Bioremediation Approaches for Clean and Green Environment

Murmu

Sevanan

2021

Strategies and Tools for Pollutant Mitigation

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Electrokinetic-enhanced bioremediation of tetrachloroethylene

Cited by 9 publications

References 39 publications

Cometabolic biodegradation of chlorinated ethenes with methanotrophs in anaerobic/aerobic simulated aquifer

Cometabolic biodegradation of chlorinated ethenes with methanotrophs in anaerobic/aerobic simulated aquifer

Complete degradation of chlorinated ethenes and its intermediates through sequential anaerobic/aerobic biodegradation in simulated groundwater columns (complete degradation of chlorinated ethenes)

Modern Bioremediation Approaches for Clean and Green Environment

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