Electrokinetic detection and separation of living algae in a microfluidic chip: implication for ship’s ballast water analysis

Song, Yongxin; Li, Zhen; Feng, Angran; Zhang, Junyan; Liu, Zhijian; Li, Dongqing

doi:10.1007/s11356-020-12315-5

Cited by 10 publications

(3 citation statements)

References 45 publications

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“…If the cells were lysed and the thylakoids exposed, the extracellular Chl- a would readily be decomposed in the ambient environment. Therefore, it is common to use Chl- a concentration as an indicator to quantify the viable cell concentration. − The Microcystis aeruginosa culture contains MC-LR, of which concentrations are batch-dependent and less predictive. Therefore, Synechococcus was used in most bench-scale tests to study the degradation kinetics of [Chl- a ] in the absence of MC-LR.…”

Section: Resultsmentioning

confidence: 97%

Mitigation of Cyanobacterial Harmful Algal Blooms (cHABs) and Cyanotoxins by Electrochemical Oxidation: From a Bench-Scale Study to Field Application

et al. 2022

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Cyanobacterial harmful algal blooms (cHABs) and the concurrent release of cyanotoxins have become an emerging threat to aquatic life and drinking water safety. It is challenging for conventional water treatment infrastructures to mitigate cHAB events that occur frequently and irregularly. This study developed an emergency-responsive electrochemical oxidation and filtration (EOF) process that enables the pump-and-treat of phytoplankton plumes and cyanotoxins. Fundamental studies indicate that the EOF process generated locally concentrated free chlorine (>16 times higher than the bulk chlorine concentration) at the porous Ti4O7 filter anode surface. Lab tests showed that cyanobacteria and the cyanotoxin microcystin-LR were inactivated and destroyed by free chlorine when passing through the porous anode. Additionally, the concentration of disinfection byproducts generated by EOF was five times less than chlorination to achieve the same level of treatment. A boat-mount full-scale EOF system was developed and deployed in Lake Neatahwanta impacted by cHAB. The system can effectively remove >50% of phytoplankton and >80% of the ambient cyanotoxins at a treatment capacity of 110 m3/day and energy consumption of 1.1 kWh/m3. Instant improvement of effluent water clarity was achieved.

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Section: Resultsmentioning

confidence: 97%

Mitigation of Cyanobacterial Harmful Algal Blooms (cHABs) and Cyanotoxins by Electrochemical Oxidation: From a Bench-Scale Study to Field Application

et al. 2022

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“…It is interesting to find that the EK velocity of algae at PEG–DEX interface is just slightly smaller than that in the PEG‐rich phase, though the DEX‐rich phase with much higher viscosity will retard the algae velocity to a large extent. In our previous study of polystyrene particle at liquid–liquid (oil–water interface), as only one side of the liquid is conducting solution, the electrophoresis is dominant in determining the EK velocity of particles [23], whereas for the algae at the PEG–DEX interface (both sides of the interface are conducting solutions), it is reported in our previous paper [18] that the surface charges (zeta potential) of dead algae decrease sharply compared with that of live algae. The reason can be attributed to the cease of metabolic process and the inactive of the anionic surface groups consequently [24].…”

Section: Resultsmentioning

confidence: 99%

“…New technology for determining the effect of ballast water treatment is still needed. For the majority of microalgae, the surfaces are negatively charged due to the anionic surface groups [18]. The difference of surface charges between live and dead microalgae makes it easier to be distinguished by electrokinetic (EK) method.…”

Section: Introductionmentioning

confidence: 99%

Electrokinetic motion and viability assessment of algae with a polyethylene glycol–dextran interface

Zhang

Cao

et al. 2023

Electrophoresis

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At present, there is still limited report on the electrokinetic (EK) behavior of bioparticles at the interface of an aqueous two‐phase system. In this paper, the EK motion and viability assessment of live algae mixed with the NaClO treated dead algae were carried out at the interface formed by polyethylene glycol (PEG)‐rich phase and dextran (DEX)‐rich phase in a straight microchannel. The experimental results show that both the live and dead algae at the PEG–DEX interface migrate from the negative electrode to the positive electrode, and the EK velocity of live algae at the interface is slightly larger than that of the dead ones with similar diameters. For either live or dead algae, the EK velocity at the interface decreases with the increase in diameter. A size–velocity curve was used to evaluate the viability of the algae. As most of the microorganisms in ballast water are algae, the method in this paper provides a promising way to detect and evaluate the live microorganism in treated ballast water of a ship.

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Modification of the electrokinetic motion of microalgae through light illumination for viability assessment

Li,

Jiang,

et al. 2024

Electrophoresis

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The viability detection of microalgae with the electrokinetic (EK) technique shows vast applications in the biology and maritime industry. However, due to the slight variations in the EK properties between alive and dead microalgae cells, the accuracy and practicability of this technique is limited. In this paper, the light illumination pretreatment was conducted to modify the EK velocity of microalgae for enhancing the EK difference. The effects of the illumination time and light color on the EK velocities of Chlorella vulgaris and Isochrysis galbana were systematically measured, and the EK differences between alive and dead cells were calculated and compared. The results indicate that under light illumination, the photosynthesis of the alive cells leads to the amplification of the zeta potential, leading toward increase in the EK difference along with the illumination time. By using light with different color spectra to treat the microalgae, it was found that the EK difference changes with the light color according to the following order: white light > red light > blue light > green light. The difference in EK potential with exposure to white light treatment surpasses over 10‐fold in comparison to those without such treatment. The light pretreatment technique, as illustrated in this study, offers an advantageous strategy to enhance the EK difference between living and dead cells, proving beneficial in the field of microalgae biotechnology.

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Electrokinetic detection and separation of living algae in a microfluidic chip: implication for ship’s ballast water analysis

Cited by 10 publications

References 45 publications

Mitigation of Cyanobacterial Harmful Algal Blooms (cHABs) and Cyanotoxins by Electrochemical Oxidation: From a Bench-Scale Study to Field Application

Mitigation of Cyanobacterial Harmful Algal Blooms (cHABs) and Cyanotoxins by Electrochemical Oxidation: From a Bench-Scale Study to Field Application

Electrokinetic motion and viability assessment of algae with a polyethylene glycol–dextran interface

Modification of the electrokinetic motion of microalgae through light illumination for viability assessment

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