Electrokinetic chromatography: An interface between electrophoresis and chromatography

Contributions to the theory and practice of the chromatographic separation of enantiomers

“…In this case, the enantioselective partitioning between a racemic selectand and the nonracemic selector does not require a physically separated stationary phase but occurs in an apparent one-phase system by a single-phase process 99 with the selector acting as a pseudostationary phase. 100 Due to enantiomerization (vide supra), the chiral tranquilizers lorazepam, oxazepam, and temazepam give rise to interconversion profiles upon enantioseparation by micellar electrokinetic chromatography (MEKC) in the presence of the charged chiral surfactant sodium cholate. 101 The interconversion profiles have been simulated by the stochastic model and by the theoretical plate model employing ChromWin 48 with excellent agreement between the two independent methods.…”

“…101 The finding that the theoretical plate model can be applied to MEKC underscores the notion of Terabe that by adding a charged component to the buffer in an electrophoretic set-up, true chromatography is created in an apparent one-phase system called electrokinetic chromatography. 100 In two-phase chromatography employing a CSP the transient diastereomers reside in the stationary phase and are immobile. By contrast, in apparent one-phase electromigration methods the transient diastereomers are not stationary but are mobile.…”

Simultaneous enantioseparation of antiparkinsonian medication Rotigotine and related chiral impurities by capillary zone electrophoresis using dual cyclodextrin system

“…The current mechanism for enantioseparation with a CD involves inclusion complexation, and the primary interaction is hydrogen bond [9]. Charged CDs often have higher chiral resolving capacity than native CDs and they were introduced as chiral selectors in CE by Terabe [10]. With charged CDs, electrostatic forces play an important role in selector-selectand interactions.…”

High-Resolution Nanotechnique for Separation, Characterization, and Quantitation of Micro- and Macromolecules

“…This technique is termed micellar electrokinetic chromatography (65) and also called micellar electrokinetic capillary chromatography (MECC) (66).…”