Electroinduction of interferon-like proteins

Pretnar, G.; Filipič, Bratko; Golob, A.; Škodič, A.; Tóth, Sz.; Mécs, I; Suhar, A.

doi:10.1016/0022-0728(91)85626-z

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…Therefore, we named them "interferon-like molecules." In our experiments, we also found [14] that medium-strength square-wave impulses of direct ionic current (DC) can result in a short-term direct antiviral resistance to virus infections and in a consequence to alter the membrane properties of the target cells [15]. We therefore decided to study the changes in the expression of membrane-bound surface marker molecules that are on the surface of the immune competent cells in the human blood [16][17][18].…”

Section: Introductionsupporting

confidence: 73%

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Square-Wave Electric Impulses of 10 ms and 100 V/cm of Field Force, Produced by PGen-1 Impulse Generator Device, Affect the Proliferation Patterns of Different Animal Cells

Filipič¹,

Gradišnik²,

Kovacs³

et al. 2020

Electromagnetic Field Radiation in Matter

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Section: Introductionsupporting

confidence: 73%

“…The picture of such a square wave is very similarly with this produced on the bioinduction device described by Pretnar et al [14] with the number of harmonics of 23.…”

Section: The Influence Of One or Three Square Impulses With A Field Fmentioning

confidence: 59%

Square-Wave Electric Impulses of 10 ms and 100 V/cm of Field Force, Produced by PGen-1 Impulse Generator Device, Affect the Proliferation Patterns of Different Animal Cells

Filipič¹,

Gradišnik²,

Kovacs³

et al. 2020

Electromagnetic Field Radiation in Matter

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show abstract

“…This collapses topically to 0 at the breached epithelium giving rise to a steady voltage gradient of 40 to 200 mV/mm directed toward the wound edge and parallel to the epithelial layer, with the wound negative about distal tissue. 3–5 Pretnar and colleagues 6 reported that one or few strong electric impulses could induce interferon (IFN)-like proteins in human leukocytes that are antigenically different from any contemporary IFN molecule. Kovacs and colleagues 7 found that in porcine leukocytes, the addition of Sendai virus and electric impulses produced porcine interferon α-like molecules.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Effect of 100.414-kHz Electromagnetic Field Produced by Defender’s Pulse Generator on the ChIFN γ-Like Molecule Inducing Capacity ofLens culinarisAgglutinin and 10% PBS Washouts of Different Holocene Minerals

Filipič¹,

Rihar²,

Gregorič³

et al. 2019

Technol Cancer Res Treat

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Macrophages play key role in host defense and tissue repair, and thus understanding regulation of their function is important. For instance, our previous results have shown that in chicken macrophage system (CoMA cell line), application of a pulse of electromagnetic fields of frequencies 0.618, 1.054, 5.229, and 100.414 kHz induces production of interferon γ-like molecules. In this study, we have shown that the electromagnetic field of 100.414 kHz is the most effective in inducing synthesis of chicken interferon γ and chicken interferon γ-like molecules in CoMA cells, especially when combined with Lens culinaris agglutinin and 10% phosphate-buffered saline washouts of different Holocene minerals. A 2-minute pulse of electromagnetic field was produced by Defender’s pulse generator. Both chicken interferon γ and chicken interferon γ-like molecules from the cell supernatant were evaluated by an antiviral assay and were also analyzed with reverse-phase high-performance liquid chromatography on Phenomenex, Aeris peptide columns. Our results show that application of a single inducing factor (Lens culinaris agglutinin, 100.414 kHz electromagnetic field, 10% phosphate buffer saline washout) or combined usage of 2 of them moderately stimulated production of chicken interferon γ-like molecules (from 1.550 to 48.028 IU/mL), whereas the combination of 10% phosphate-buffered saline washout of Koprivnica rock + Lens culinaris agglutinin + 100.414 kHz/9 V resulted in an output of 162.122 IU/mL. Hence, we may conclude that a combined use of electromagnetic field, Holocene minerals, and Lens culinaris agglutinin greatly stimulates synthesis of chicken interferon γ-like molecules in CoMA cells.

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Purification of an atypical bovine interferon induced in response to heat shock in bovine cultured cells. Characterization in comparison with the classical α, β and γ interferon

1997

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Electroinduction of interferon-like proteins

Cited by 3 publications

References 18 publications

Square-Wave Electric Impulses of 10 ms and 100 V/cm of Field Force, Produced by PGen-1 Impulse Generator Device, Affect the Proliferation Patterns of Different Animal Cells

Square-Wave Electric Impulses of 10 ms and 100 V/cm of Field Force, Produced by PGen-1 Impulse Generator Device, Affect the Proliferation Patterns of Different Animal Cells

Enhancing Effect of 100.414-kHz Electromagnetic Field Produced by Defender’s Pulse Generator on the ChIFN γ-Like Molecule Inducing Capacity ofLens culinarisAgglutinin and 10% PBS Washouts of Different Holocene Minerals

Purification of an atypical bovine interferon induced in response to heat shock in bovine cultured cells. Characterization in comparison with the classical α, β and γ interferon

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