Electroimmunoblotting of small peptides Separated on Urea-Dodecyl Sulphate (SUDS) gels application to myelin basic protein

Immunoblotting and dot blotting

“…The epitopes of various proteins have been mapped and, in some cases, correlated with bio- Luzio and Jackson (1988) Jarausch and Kadenbach (1985) Vartio et al (1982); Dziadek et al (1983) Cohen et al (1986) Costa et al (1986) Mendel-Hartvig and Nelson (1983) Sheng et al (1988) Samson (1986) Yurchenco et al (1982 Reiser and Wardale (1981) Delia et al (1987) Dunn et al ( ) King et al (1985; Cevenini et al (1986); Caldwell et al (1987); Patel et al (1988); Heard et al (1986); Rautenberg et al (1986) Swanson andBarrera (1983); Hadfield and Glynn (1984); Schalla et al (1986) Chapman et al (1987; Kelson et al (1988) De Jongh Leuvenink et al (1985; Sturm et al (1984) Coil et al (1986); Wedege and Froholm (1986); Wedege et al (1988) Aznar et al (1988) Andersen et al (1986; Coates et al (1986);Milner et al (1987); Van Vooren et al (1988) Kermy and Cartwright (1984); Kibe et al (1985); Jacobs et al (1986); Andersen et al (1987); Sasaki et al (1987); Young and Ross ( Battaglia et al…”

A peptide of human muscarinic acetylcholine receptor 3 is antigenic in primary Sjögren's syndrome

“…Peptide was transferred from the gel to nitrocellulose membrane (0.2 mm). Transfers were carried out according to [12] with minor modifications, in 25 mM sodium phosphate buffer, pH 6.8 containing 10% methanol, at 20 V for 2 h. The membrane was treated with 0.2% glutaraldehyde for 30 min prior to the quenching step in SuperBlock Blocking Buffer. The membrane was reacted with patient's serum (1:500) and then handled as described above.…”

Opioid Activity of β‐Endorphin‐like Proteins from Tetrahymena

“…Proteins from fractions obtained from Sep‐Pack C 18 columns were resolved using SDS‐Urea gels (SUDS), 17.5% acrylamide, as described by other investigators (Kyte and Rodríguez 1983; Sheng et al 1988). These gels permit detection of small polypeptides, such as β‐endorphin.…”