Electrohydrodynamic drying of probiotics

Dima, Panagiota; Gulbinas, Giedrius; Stubbe, P.; Mendes, Ana Carina Loureiro; Chonakis, Ioannis S.

doi:10.1016/j.ifset.2022.103201

Cited by 10 publications

(2 citation statements)

References 57 publications

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“…The probiotic cells’ surface electrical charge evaluation was performed according to a previously described protocol ( Dertli et al, 2015 ; Dima et al, 2022 ). Approximately 10 8 ST44 probiotic cells/mL were suspended in 10 mM KH 2 PO 4 (Sigma-Aldrich, Steinheim, Germany) and transferred to a capillary cell with gold plated beryllium/copper electrodes (DTS1070 cell, Malvern Panalytical Ltd, Malvern, UK).…”

Section: Methodsmentioning

confidence: 99%

Enhanced electric field and charge polarity modulate the microencapsulation and stability of electrosprayed probiotic cells (Streptococcus thermophilus, ST44)

Dima,

Stubbe,

Mendes

et al. 2023

Current Research in Food Science

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Section: Methodsmentioning

confidence: 99%

Enhanced electric field and charge polarity modulate the microencapsulation and stability of electrosprayed probiotic cells (Streptococcus thermophilus, ST44)

Dima,

Stubbe,

Mendes

et al. 2023

Current Research in Food Science

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“…The probiotic cells' surface electrical charge evaluation was performed according to a previously described protocol (Dertli et al 2015;Dima, Gulbinas, et al 2022). Approximately 10 8 ST44 probiotic cells/mL were suspended in 10 mM KH 2 PO 4 (Sigma-Aldrich, Steinheim, Germany) and transferred to a capillary cell with gold plated beryllium/copper electrodes (DTS1070 cell, Malvern Panalytical Ltd, Malvern, UK).…”

Section: Bacterial Cell Surface Chargementioning

confidence: 99%

Enhanced electric field and charge polarity modulate the microencapsulation and stability of electrosprayed probiotic cells (Streptococcus thermophilus, ST44)

Dima

Stubbe

Mendes

et al. 2023

Preprint

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The “organization” of Streptococcus thermophilus (ST44) probiotic cells within maltodextrin microcapsules was investigated, using electrospray processing. The generated electrostatic forces between the negatively surface-charged probiotic cells and the applied negative polarity on the electrospray nozzle, allowed to control the location of the cells towards the core of the electrosprayed microcapsules. The “organization” of the cells affected the evaporation of the solvent (water) and subsequently the glass transition temperature (Tg) of the electrosprayed microcapsules. Moreover, the utilization of auxiliary ring-shaped electrodes, between the nozzle and the collector, enhanced the electric field strength and contributed further to the increase of the Tg. Numerical simulation, through Finite Element Method (FEM), shed light to the effects of the additional ring-electrode on the electric field strength, potential distribution, and controlled deposition of the capsules. Moreover, the viability of the encapsulated cells was significantly improved for up to 2 weeks of storage at 25°C and 35% RH, when the cells were located at the core of the microcapsules, compared to the probiotics distributed towards the surface. Overall, this study presents a novel method to manipulate the encapsulation of the surface charged probiotic cells within electrosprayed microcapsules, utilizing the polarity of the electric field and additional ring-electrodes.

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Enhanced production rate of probiotic Enterococcus faecium A028 by repeated batch solid-state fermentation and its survival under hot air drying with cell-protective agents

Kannika,

Teeka,

Kaewpa

et al. 2023

Biocatalysis and Agricultural Biotechnology

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Electrohydrodynamic drying of probiotics

Cited by 10 publications

References 57 publications

Enhanced electric field and charge polarity modulate the microencapsulation and stability of electrosprayed probiotic cells (Streptococcus thermophilus, ST44)

Enhanced electric field and charge polarity modulate the microencapsulation and stability of electrosprayed probiotic cells (Streptococcus thermophilus, ST44)

Enhanced electric field and charge polarity modulate the microencapsulation and stability of electrosprayed probiotic cells (Streptococcus thermophilus, ST44)

Enhanced production rate of probiotic Enterococcus faecium A028 by repeated batch solid-state fermentation and its survival under hot air drying with cell-protective agents

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