Electrogenerated chemiluminescence detection of single entities

Zhao, Wei; Chen, Hong‐Yuan; Xu, Jing‐Juan

doi:10.1039/d0sc07085h

Cited by 100 publications

(72 citation statements)

References 123 publications

(151 reference statements)

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“…6 To enable greater ECL efficiency 14 and brighter ECL (nano)-emitters with tuneable emission colours, many works have been carried out with original materials and novel detection schemes. 1,[15][16][17] With the continuous rise of semiconductor (SC) (nano) materials science and its miniaturization, the combination of ECL with SC materials offers the possibility to design ECL readout strategies with improved performances. For example, the eld of SC photoelectrochemistry is extremely active for solar energy conversion, analytical chemistry, and spatially resolved photoelectrochemistry.…”

Section: Introductionmentioning

confidence: 99%

Electrochemiluminescence with semiconductor (nano)materials

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Electrochemiluminescence with semiconductor (nano)materials

et al. 2022

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“…Thus, ROS-triggered ECL would be a facile and highly sensitive tool to screen kinetics of ORR electrocatalysts; however, except for few works on ECL-imaging of single particles qualitatively acquiring active site distribution, it has been rarely explored. 33 Herein, we report a ROS-oriented strategy for kinetic evaluation of ORR electrocatalysts using ultrasensitive time resolved ECL (Tr-ECL) in a conventional three-electrode electrochemical cell configuration. It consisted of synchronous ECL intensity collection and sequential ORR/ECL reactions driven by double-potential step chronoamperometry.…”

Section: Introductionmentioning

confidence: 99%

Elucidating Electrocatalytic Oxygen Reduction Kinetics via Intermediates by Time Resolved Electrochemiluminescence

Wu¹,

Chen²,

Chen³

et al. 2022

Preprint

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Rapid and accurate evaluation of oxygen reduction reaction (ORR) kinetics for massive potential electrocatalysts is critical for developing sustainable energy conversion devices. In principle, both the consumption rate of O2 and production rate of reactive oxygen radicals (ROS) intermediates can be used to establish the ORR rate equation. Notably, owing to the grand challenge in quantitative in-situ detection of trace-amount of ROS, most previous ORR kinetics studies rely on direct electrochemical examination of O2 consumption, but which is inevitably hampered by the slow mass transfer of O2 in electrolytes. Here we report a time resolved electrochemiluminescence (Tr-ECL) strategy to establish ORR rate equation via ultra-sensitive detection of ROS intermediates. As ROS were generated at electrocatalysts surfaces in the diffusion layer, it intrinsically circumvented the limit of mass transfer of O2 in electrolytes during ORR. As a result, ORR electron transfer numbers, rate constants, and ROS concentration-potential correlations that is closely related to the stability of electrocatalysts were successfully obtained by principal component analysis of ECL intensities and finite elemental analysis of time-resolved ECL decay curves. It provides a rapidly, precisely, and facile way to understand ORR mechanism and would greatly pave development of electrocatalysts with appropriate catalytic activity, selectivity and stability.

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“…Electrochemiluminescence (ECL) is the emission of light triggered by electrochemical reactions, which shows unique advantages, such as low background noise, high sensitivity, and maneuverability. − Benefiting from the superiority of ECL and the specificity of immunoreaction, the ECL immunoassay (ECLIA) has become a powerful analytical tool for helping overcome the limitations of the enzyme-linked immunosorbent assay. − However, the complex preparation of the antibody-tagged ECL probe and ECLIA platform limits their further development. Moreover, because of the low sensitivity of directly labeled antibodies, various tedious signal amplification strategies have been reported. − In addition, the common sandwich-type ECLIA mode is usually an “on-electrode” state since a large number of labels are outside the Helmholtz plane surface, resulting in a less sensitive detection. − Therefore, exploring a novel, convenient, and advanced multifunctional ECLIA probe to surmount these problems is still an active and continuous key aim for this issue.…”

Section: Introductionmentioning

confidence: 99%

Electrochemiluminescence Immunoassay Platform with Immunoglobulin G-Encapsulated Gold Nanoclusters as a “Two-In-One” Probe

Hong

Huang

et al. 2021

Anal. Chem.

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Biomolecule-functionalized Au nanoclusters (AuNCs) have drawn great interest in the electrochemiluminescence (ECL) field due to their unique optical/electrical properties, biocompatibility, and versatile bioapplication potentials. Herein, we proposed a two-in-one ECL probe of immunoglobulin Gencapsulated AuNCs (IgG-AuNCs) for the development of a highperformance ECL immunoassay (ECLIA) platform. The IgG-AuNCs were not only used as an ECL probe due to their excellent anodic ECL performance with triethylamine (TEA) as a coreactant but also used as the biorecognition element because of their wellretained bioactivity of the IgG. As a proof of concept, a new type of competitive immunosensing platform has been applied to detect IgG representing several merits of facile preparation, rapid detection, sample saving, and good analytical performance. The sensing platform exhibited a linear range of 0.5−50,000 ng/mL with a limit of detection of 0.06 ng/mL for IgG detection with high selectivity. In addition, this convenient ECLIA platform also performed well in real serum sample detection. Notably, our work not only proved the "two-in-one" immuno-AuNC probe-based ECLIA strategy but also developed a rational framework for study of ECL bioassay platforms based on multifunctional AuNCs and other related nanomaterials.

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Electrogenerated chemiluminescence detection of single entities

Abstract: We summarize the history and recent development that has been made in the ECL detection of single entities.

Cited by 100 publications

References 123 publications

Electrochemiluminescence with semiconductor (nano)materials

Electrochemiluminescence with semiconductor (nano)materials

Elucidating Electrocatalytic Oxygen Reduction Kinetics via Intermediates by Time Resolved Electrochemiluminescence

Electrochemiluminescence Immunoassay Platform with Immunoglobulin G-Encapsulated Gold Nanoclusters as a “Two-In-One” Probe

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