Electrofusion preparation of anti-triazophos monoclonal antibodies for development of an indirect competitive enzyme-linked immunosorbent assay

Song, Guangyue; Huang, Lianrun; Huang, Yue; Liu, Weimei; Wang, Minghua; Hua, Xiude

doi:10.1016/j.jim.2021.113184

Cited by 5 publications

(14 citation statements)

References 28 publications

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“…used the ratio of the number of successfully fused hybridoma colonies to the number of lymphocytes as the fusion yield. Song et al [44] . and Ke et al [38] .…”

Section: Calculation Of Fusion Yieldmentioning

confidence: 99%

“…There are diameter differences between the two cell lines: diameters of myeloma cells and spleen cells are about 12 μm and 7 μm, respectively [42] . This complicates the choice of the optimal electric field for fusion of these cells since different electric field intensities are required to perforate membranes of different cell lines [43,44] . In order to address this problem, Kato et al [42] .…”

Section: Change Cell Sizementioning

confidence: 99%

“…Electrofusion was then carried out in a 2-mL chamber, and satisfactory electrofusion results were also obtained. Song [44] and Cold Spring Harbor Laboratory [47] also performed electrofusion in a 2-mL electrofusion chamber with the number of cells maintained at 1×10 6 . They utilized spleen-derived lymphocytes and myeloma cells at a 1:1 proportion and also achieved satisfactory fusion results.…”

Section: Control Cell Number and Proportionmentioning

confidence: 99%

“…In this study, the authors believed that the regular arrangement of cells in a relatively long pearl-chain (Figure . 5A3 ) was the most suitable cell state for electrofusion. In 2022, Song et al [44] . also observed the state of cell alignment under an optical microscope (Figure .…”

Section: Observation and Evaluation Of Cell Statementioning

confidence: 99%

“…Part A is reprinted with permission from Yin et al(29). Part B is reprinted with permission from Song et al[44]. Part C is reprinted with permission from Wu et al[46].…”

mentioning

confidence: 99%

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Advances in hybridoma preparation using electrofusion technology

Kou

Shen

wang

et al. 2022

Preprint

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As a rapidly developing cell engineering technique, cell electrofusion has been increasingly applied in the field of hybridoma preparation in recent years. However, electrofusion is a certain degree of difficulty to completely replace the polyethylene glycol-mediated cell fusion. The key elements limiting electrofusion in the field of hybridoma preparation are practical complicated. This review summarizes the state of art of cell electrofusion in hybridoma preparation based on recent published literatures, mainly focusing on electrofusion instruments and their components, process control, cell treatment, and process characterization. The review provides new information and insightful commentary, critically important to the promotion of further electrofusion development in the field of hybridoma preparation.

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“…used the ratio of the number of successfully fused hybridoma colonies to the number of lymphocytes as the fusion yield. Song et al [44] . and Ke et al [38] .…”

Section: Calculation Of Fusion Yieldmentioning

confidence: 99%

Section: Change Cell Sizementioning

confidence: 99%

Section: Control Cell Number and Proportionmentioning

confidence: 99%

Section: Observation and Evaluation Of Cell Statementioning

confidence: 99%

“…Part A is reprinted with permission from Yin et al(29). Part B is reprinted with permission from Song et al[44]. Part C is reprinted with permission from Wu et al[46].…”

mentioning

confidence: 99%

See 3 more Smart Citations

Advances in hybridoma preparation using electrofusion technology

Kou

Shen

wang

et al. 2022

Preprint

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show abstract

Advances in hybridoma preparation using electrofusion technology

Kou

Shen

Wang

et al. 2023

Biotechnology Journal

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As a rapidly developing cell engineering technique, cell electrofusion has been increasingly applied in the field of hybridoma preparation in recent years. However, it is difficult to completely replace the polyethylene glycol‐mediated cell fusion using electrofusion due to the high operation requirements, high cost of electrofusion instruments, and lack of prior reference research work. The key elements limiting electrofusion in the field of hybridoma preparation also introduce practical complications, such as the use/choice of electrofusion instruments, setup/optimization of electrical parameters, and precise control of cells. This review summarizes the state of the art of cell electrofusion in hybridoma preparation based on recent published literature, mainly focusing on electrofusion instruments and their components, process control and characterization, and cell treatment. It also provides new information and insightful commentary critically important for further electrofusion development in the field of hybridoma preparation.

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Rapid Detection of Prometryn Residues in Agricultural Samples: Method Comparison of the ic-ELISA and Colloidal Gold Immunochromatographic Test Strips

Gao,

Abd El-Aty,

et al. 2024

ACS Agric. Sci. Technol.

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This study aimed to establish two methods, namely, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold immunochromatographic test strips, for detecting prometryn residues in various agricultural samples, such as corn, pumpkin, lotus root, rice, and brown rice. Monoclonal antibodies specific for prometryn were utilized in both methods. The ic-ELISA method demonstrated a maximum half-inhibition concentration (IC 50 ) of 2.4 ng/mL and a lower limit of detection of 0.5 ng/mL. When used in conjunction with a card reader, the colloidal gold immunochromatographic test strip exhibited an IC 50 of 18.6 ng/mL for prometryn detection. The recovery rates of both methods were found to be consistent with those of liquid chromatography tandem mass spectrometry. This consistency indicated the suitability of both methods for detecting prometryn in five different agricultural samples, suggesting that this is a convenient and practical approach for screening prometryn in agricultural products.

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Electrofusion preparation of anti-triazophos monoclonal antibodies for development of an indirect competitive enzyme-linked immunosorbent assay

Cited by 5 publications

References 28 publications

Advances in hybridoma preparation using electrofusion technology

Advances in hybridoma preparation using electrofusion technology

Advances in hybridoma preparation using electrofusion technology

Rapid Detection of Prometryn Residues in Agricultural Samples: Method Comparison of the ic-ELISA and Colloidal Gold Immunochromatographic Test Strips

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