Electroejaculation and semen analysis in a male lowland gorilla,Gorilla gorilla gorilla

Platz, C. C.; Wildt, David E.; Bridges, C. H.; Seager, S. W. J.; Whitlock, B. S.

doi:10.1007/bf02383831

Cited by 22 publications

(11 citation statements)

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“…Studies of semen characteristics in aging nonhuman primates have been cross-sectional, often with single or short time-point measures and small sample sizes (Platz et al 1980;Tollner et al 1990;Schaffer et al 1992;VandeVoort et al 1993;Morrell 1997;Yeoman et al 1997;Ramesh et al 1998;Ji et al 2001). Some studies have focused on testosterone levels in male rhesus macaques, with somewhat contradictory results, similar to the human data (Mattison et al 2001;Black and Lane 2002;Mattison et al 2003;Roth et al 2004).…”

Section: Nonhuman Primate Modelsmentioning

confidence: 99%

Aging in male primates: reproductive decline, effects of calorie restriction and future research potential

Sitzmann

Urbanski

Ottinger

2008

AGE

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Although less dramatic than in females, male mammals experience decreasing reproductive function during aging. In primates, multiple facets of the hypothalamic-pituitary-gonadal axis show evidence of gradual age-related decline, including behavioral, neuroendocrine and endocrine alterations such as decreased testosterone levels, reduced circulating dehydroepiandrosterone sulfate (DHEAS) levels, increased numbers of sperm abnormalities, and a general decline in physiological responses. In this review we consider a range of age-related changes in males. These measures, including more subtle aging characteristics, are interesting additional indices for detecting the timing of age-related changes in behavioral, neuroendocrine, and endocrine responses. Evidence of potential effects of calorie restriction as an intervention in reproductive aging is also discussed. A discernable decline occurs in both metabolic and reproductive endocrine processes during male aging. This cascade of events includes neuroendocrine and behavioral changes; biomarkers such as circulating DHEAS also show clear age-related decline. The varied changes that occur during male aging are considered in the context of primate aging in general.

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Section: Nonhuman Primate Modelsmentioning

confidence: 99%

Aging in male primates: reproductive decline, effects of calorie restriction and future research potential

Sitzmann

Urbanski

Ottinger

2008

AGE

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“…Sperm pathologies such as teratozoospermia are often observed in primates including humans (Platz et al. ; Cui et al. ; Baccetti et al.…”

Section: Introductionmentioning

confidence: 99%

“…In mammals, including primate species, sperm pathologies are frequently associated with marked or subtle changes in sperm morphology (Chemes and Rawe 2003;Valle et al 2012). Sperm pathologies such as teratozoospermia are often observed in primates including humans (Platz et al 1980;Cui et al 1991;Baccetti et al 2002;Nakazato et al 2015). Traditionally, sperm morphology analyses have been carried out using subjective diagnostic methods to classify amorphous sperm heads, midpieces and tails.…”

Section: Introductionmentioning

confidence: 99%

Sperm Morphology Assessment in Captive Neotropical Primates

Swanson

Valle

Carvalho

et al. 2016

Reprod Domestic Animals

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The main objective of this study was to evaluate sperm morphology in four neotropical primate species to compare the sperm morphological traits and the sperm morphometric parameters as a basis for establishing normative sperm standards for each species. Data from 80 ejaculates collected from four primate species, Callithrix jacchus, Callimico goeldii, Alouatta caraya and Ateles geoffroyi, were analysed for detection of sperm morphological alterations using subjective World Health Organization (WHO-2010) standards and Sperm Deformity Index (SDI) criteria, objective computer-assisted sperm morphometry analysis (CASMA) and subpopulation sperm determination (SSD) methods. There were multiple differences (p < 0.01) observed among primate species in values obtained from WHO-2010, SDI, CASMA and SSD sperm analysis methods. In addition, multiple significant positive and negative correlations were observed between the sperm morphological traits (SDI, Sperm Deformity Index Head Defects, Sperm Deformity Index Midpiece Defects, Sperm Deformity Index Tail Defects, Normal Sperm, Head Defects, Midpiece Defects and Tail Defects) and the sperm morphometric parameters (SSD, Area (A), Perimeter (P), Length (L), Width (W), Ellipticity, Elongation and Rugosity) (p ≤ 0.046). In conclusion, our findings using different evaluation methods indicate that pronounced sperm morphological variation exists among these four neotropical primate species. Because of the strong relationship observed among morphological and morphometric parameters, these results suggest that application of objective analysis methods could substantially improve the reliability of comparative studies and help to establish valid normative sperm values for neotropical primates.

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“…Spermatozoa used in previous gorilla ART studies were collected either by electroejaculation using a rectal probe or from excised epididymides. Characteristically, gorilla semen collected by electrostimulation is of low motility and often has a high percentage of abnormal spermatozoa [Platz et al, 1980;Gould & Kling, 1982;Schaffer et al, 1989]. Therefore, in the present study, a young, reproductively naive gorilla male was conditioned to ejaculate by manual penile stimulation.…”

Section: Introductionmentioning

confidence: 90%

Birth of a Western Lowland gorilla (Gorilla gorilla gorilla) following in vitro fertilization and embryo transfer

et al. 1997

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A 21‐year‐old multiparous female exhibiting 31–41 day menstrual cycles was given hFSH (225 IU/day, Metrodin 75, from cycle day 3 through 9 (menses = day 1) and hCG (10,000 IU, Profasi, on day 10 to stimulate follicular development. At 35 h after hCG, under isoflurane (AErrane) anesthesia, follicles were aspirated by controlled suction under transvaginal ultrasound guidance. Metaphase II oocyctes (n = 11) were placed in modified human tubal fluid (mHTF, 100 μl) medium under oil at 37°C in humidified 5% CO2. Frozen semen, collected by voluntary ejaculation, was thawed (70°C H2O bath, 6 sec), diluted slowly, centrifuged, and resuspended in mHTF, and 160,000 motile spermatozoa/ml were added at 6 h after oocyte recovery. At 21 h postinsemination (p.i.) eight oocytes were at the two‐cell stage, five were cryopreserved, and three were cultured to the six‐ to eight‐cell stage in mHTF with granulosa cells before transcervical uterine transfer at 47 h p.i. using a Teflon catheter. Micronized progesterone (400 mg/d) was orally administered for 10 weeks posttransfer (p.t.). Ultrasound examination revealed a single fetus at 15 weeks p.t., and unassisted delivery of a live 1.37 kg female infant occurred at 29 weeks. Am. J. Primatol. 41:247–260, 1997. © 1997 Wiley‐Liss, Inc.

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Electroejaculation and semen analysis in a male lowland gorilla,Gorilla gorilla gorilla

Cited by 22 publications

References 0 publications

Aging in male primates: reproductive decline, effects of calorie restriction and future research potential

Aging in male primates: reproductive decline, effects of calorie restriction and future research potential

Sperm Morphology Assessment in Captive Neotropical Primates

Birth of a Western Lowland gorilla (Gorilla gorilla gorilla) following in vitro fertilization and embryo transfer

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