Background: Electrodermal measurement of acupuncture points has been correlated to physiologic and pathologic conditions with clinical and laboratory studies. Emerging evidence indicates that in addition to therapeutic effect, acupuncture points may also have diagnostic properties. However, physiologic basis of this phenomenon is still unknown. Objective: To determine how electrodermal activity of acupuncture points on hands and feet responds to exercise-induced sympathetic stress and how electrodermal measurement correlates to skin temperature. Materials and Methods: A 20-minute running exercise with a moderate intensity based on Borg Rating of Perceived Exertion Scale was carried out with one healthy male subject for 15 sessions. Electrodermal activity of acupuncture points was tested before and after exercise with a meridian testing system, Electroacupuncture According to Voll. Bio signs including heart rate,