Electrodeposition of calcium phosphate onto polyethylene terephthalate artificial ligament enhances graft-bone integration after anterior cruciate ligament reconstruction

Cai, Jiangyu; Zhang, Qianqian; Chen, Jiebo; Jiang, Jia; Mo, Xiumei; He, Chuanglong; Zhao, Jinzhong

doi:10.1016/j.bioactmat.2020.08.037

Cited by 29 publications

(19 citation statements)

References 35 publications

(45 reference statements)

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“…To detect new bone ingrowth into the bone tunnels, the mean cross-sectional areas of both the femoral and tibial tunnels were calculated (Appendix 1 Figure A2, available online). 4,39 Moreover, the bone volume/total volume (BV/TV) ratios of both tunnels were further analyzed quantitatively, and the volumes of interest of the tibial and femoral tunnels represented 2 cylinders on the 3-dimensional CT images, with a mean height of 130 sections (2.34 mm) and a diameter of 1.5 mm (Appendix Figure A4, available online). 3…”

Section: Micro-ct Scanningmentioning

confidence: 99%

Infrapatellar Fat Pad Mesenchymal Stromal Cell–Derived Exosomes Accelerate Tendon-Bone Healing and Intra-articular Graft Remodeling After Anterior Cruciate Ligament Reconstruction

Han

et al. 2022

Am J Sports Med

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Background: Exosomes derived from mesenchymal stromal cells (MSCs) reportedly enhance the healing process. However, no studies have investigated the effect of exosomes from infrapatellar fat pad (IPFP) MSCs on tendon-bone healing and intra-articular graft remodeling after anterior cruciate ligament reconstruction (ACLR). Purpose: To evaluate the in vivo effect of exosomes from IPFP MSCs on tendon-bone healing and intra-articular graft remodeling in a rat model of ACLR. Study Design: Controlled laboratory study. Methods: A total of 90 skeletally mature male Sprague Dawley rats underwent unilateral ACLR using an autograft. All rats were randomly divided into 3 groups: sham injection (SI) group (n = 30), control injection (CI) group (n = 30), and IPFP MSC–derived exosome injection (IMEI) group (n = 30). At 2, 4, and 8 weeks postoperatively, tendon-bone healing and intra-articular graft remodeling were evaluated via biomechanical testing, micro–computed tomography, and histological analysis; macrophage polarization was evaluated using immunohistochemical staining. Results: Biomechanical testing demonstrated a significantly higher failure load and stiffness in the IMEI group than in the SI and CI groups at 4 and 8 weeks postoperatively. Moreover, a thinner graft-to-bone healing interface with more fibrocartilage was observed in the IMEI group at both time points. Micro–computed tomography revealed greater new bone ingrowth in the IMEI group than in the other groups, as demonstrated by smaller mean bone tunnel areas and a larger bone volume/total volume ratio. Additionally, more cellular infiltration was observed in the intra-articular graft in the IMEI group than in the other groups at 4 weeks, followed by more regularly organized fibers with mature collagen at 8 weeks. Notably, similar trends of macrophage polarization were found at both the graft-to-bone interface and the intra-articular graft in the IMEI group, with significantly fewer proinflammatory M1 macrophages and larger numbers of reparative M2 macrophages than in the SI and CI groups. Conclusion: IPFP MSC–derived exosomes accelerated tendon-bone healing and intra-articular graft remodeling after ACLR, which may have resulted from the immunomodulation of macrophage polarization. Clinical Relevance: The IPFP can be easily harvested by most orthopaedic surgeons. Exosomes from IPFP MSCs, constituting a newly emerging cell-free approach, may represent a treatment option for improving tendon-bone healing and intra-articular graft remodeling after ACLR.

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Section: Micro-ct Scanningmentioning

confidence: 99%

Infrapatellar Fat Pad Mesenchymal Stromal Cell–Derived Exosomes Accelerate Tendon-Bone Healing and Intra-articular Graft Remodeling After Anterior Cruciate Ligament Reconstruction

Han

et al. 2022

Am J Sports Med

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“…The healing at the tendon–bone interface occurs most frequently by forming of fibrovascular scar tissues rather than reforming a native entheses with distinct areas of fibrocartilage transition [ 25 , 26 ]. The fibrovascular scar interface lacked the integrity of the native fibrocartilaginous entheses, resulting in the mechanical and structural inferiority [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

High-purity magnesium screws modulate macrophage polarization during the tendon–bone healing process in the anterior cruciate ligament reconstruction rabbit model

Peng

Weng

Hamushan

et al. 2022

Regenerative Biomaterials

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Magnesium (Mg) screws perform clinical potential in anterior cruciate ligament (ACL) reconstruction, and promote fibrocartilaginous entheses regeneration at the femoral entrance. We aim to prove that high-purity Magnesium (HP Mg) screws modulate macrophage polarization in fibrocartilage interface regeneration both in vitro and in vivo. HP Mg extracts performed good cytocompatibility and significantly promoted M2 macrophage polarization in the flow cytometry and ELISA assays. M2 macrophages stimulated fibrochondrocyte differentiation of co-cultured hBMSCs, and HP Mg extracts had synergistic effect on the process. Then we applied HP Mg screws, with Ti screws as control, in the ACL reconstruction rabbit model. In the histological and immunofluorescence analysis, HP Mg screws inhibited M1 polarization at 2 weeks and highly promoted M2 polarization at 2 and 4 weeks at the tendon-bone interface. Furthermore, regeneration of fibrocartilaginous entheses, rather than the fibrovascular scar interface, was detected in the HP Mg group at 12 weeks. For further mechanism study via RNA-seq detection and WB assays, we found that AKT1 was highly activated in M2 polarization, and HP Mg could stimulate AKT1 expression, rather than AKT2, in the early phase of tendon-bone healing. Our study elucidated macrophage polarization during tendon-bone healing process and emphasized HP Mg on M2 polarization and fibrocartilage interface regeneration via the selective activation of AKT1 and PI3K/AKT pathway.

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“…The commonly used surface modification methods can be divided into the additive modification and subtractive modification [ 16 ]. Additive modification refers to the addition of extra materials to implants, including inorganic/organic coating, growth factor, active ion, etc., in order to enhance the bioactivity of implants [ 17 , 18 , 19 ]. Subtractive modification methods (including anodic oxidation, sandblasting, laser treatment, acid–alkali treatment, etc.)…”

Section: Introductionmentioning

confidence: 99%

Construction of Local Drug Delivery System on Titanium-Based Implants to Improve Osseointegration

et al. 2022

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Titanium and its alloys are the most widely applied orthopedic and dental implant materials due to their high biocompatibility, superior corrosion resistance, and outstanding mechanical properties. However, the lack of superior osseointegration remains the main obstacle to successful implantation. Previous traditional surface modification methods of titanium-based implants cannot fully meet the clinical needs of osseointegration. The construction of local drug delivery systems (e.g., antimicrobial drug delivery systems, anti-bone resorption drug delivery systems, etc.) on titanium-based implants has been proved to be an effective strategy to improve osseointegration. Meanwhile, these drug delivery systems can also be combined with traditional surface modification methods, such as anodic oxidation, acid etching, surface coating technology, etc., to achieve desirable and enhanced osseointegration. In this paper, we review the research progress of different local drug delivery systems using titanium-based implants and provide a theoretical basis for further research on drug delivery systems to promote bone–implant integration in the future.

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Electrodeposition of calcium phosphate onto polyethylene terephthalate artificial ligament enhances graft-bone integration after anterior cruciate ligament reconstruction

Cited by 29 publications

References 35 publications

Infrapatellar Fat Pad Mesenchymal Stromal Cell–Derived Exosomes Accelerate Tendon-Bone Healing and Intra-articular Graft Remodeling After Anterior Cruciate Ligament Reconstruction

Infrapatellar Fat Pad Mesenchymal Stromal Cell–Derived Exosomes Accelerate Tendon-Bone Healing and Intra-articular Graft Remodeling After Anterior Cruciate Ligament Reconstruction

High-purity magnesium screws modulate macrophage polarization during the tendon–bone healing process in the anterior cruciate ligament reconstruction rabbit model

Construction of Local Drug Delivery System on Titanium-Based Implants to Improve Osseointegration

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