Electrodeposition of anchored polypyrrole film on microelectrodes and stimulation of cultured cardiac myocytes

A contactless electrical stimulator: application to fabricate functional skeletal muscle tissue

“…2-(c)). This gas evolution was also observed in our previous work where Pt microelectrodes were employed to stimulate cardiac myocytes (Nishizawa et al 2008). Finally, it seems that the ES through control device for 24 h caused Pt corrosion and damage to the device (Fig.…”

Electrical Stimulation in Tissue Regeneration

“…Lohmann reported that the levels of connection of Cx43 protein were reduced in both MLO-Y4 cells and ROS 17/2.8 cells under low frequency EMF (Lohmann et al, 2003). When applying ES (interval 1 s, duration 100 ms, amplitude 10 mA) to myocytes, Nishizawa et al (Nishizawa et al, 2007) used a high-speed confocal microscope to analyze cellular communication within the myocytes and discovered that cytosolic Ca 2+ concentration had been upregulated, which indicates that gap junctions largely contributed to the propagation of intracellular signals. Gap junctions also play a crucial role in the response of cellular networks to extracellular signals through the integration and amplification of the signals.…”

“…Despite these and many other observations showing the beneficial effect of ES to myocardium and cardiac muscle cells, the application of conductive polymers in cardiac tissue engineering remains very limited. In 2007, Nishizawa et al (Nishizawa et al, 2007) applied external ES to cultured cardiac myocytes using a microelectrode made of PPy-coated polyimide substrate. In their experiment, the myocytes were cultured on the PPy-coated anodic electrode, with the cathodic Pt immersed in the culture media over the cells.…”

Facile Synthesis of Conductive Polypyrrole Wrinkle Topographies on Polydimethylsiloxane via a Swelling–Deswelling Process and Their Potential Uses in Tissue Engineering

“…7 In particular, CP-based have been shown to be useful to electrically stimulate various cells, including neurons and cardiomyocytes, and electrically deliver biological molecules to modulate cellular behaviors for potential tissue engineering applications. [8][9][10][11] For example, electrical stimulation via various CP-based scaffolds (e.g., films, nanofibrous meshes, and porous substrates) could promote neurite outgrowth of neuronal cells. 12,13 Furthermore, multifunctional CP biomaterials have been studied to improve cell and CP materials interactions by incorporating various important properties (e.g., bioactivity, porosity, and topography) into CP materials.…”