Electrode-supported biomimetic membranes: An electrochemical and surface science approach for characterizing biological cell membranes

Su, Zhixun; Leitch, J. Jay; Lipkowski, Jacek

doi:10.1016/j.coelec.2018.05.020

Cited by 35 publications

(36 citation statements)

References 162 publications

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“…In the recent years, a combination of traditional electrochemical techniques and surface sensitive methods, such as spectroscopy, neutron scattering, and microscopic methods, have been employed to understand the behavior and structure of these biomimetic systems. On gold surfaces, it is possible to build different types of biomimetic bilayers ( Figure 2), such as metal supported bilayer lipid membranes (sBLMs), tethered bilayer lipid membranes (tBLMs), or floating bilayer lipid membranes (fBLMs) [19,20]. Metal supported lipid membranes (sBLMs) (Figure 2a) were the first generation of lipid bilayer system used to mimic and understand the cell membrane processes.…”

Section: Biomimetic Membranes On Electrodesmentioning

confidence: 99%

“…The effect of the electric field has effectively been studied with fundamental electrochemical techniques, such as differential capacitance and charge density. These results allow for the characterization of sBLMs providing information regarding the quality, compactness, and defect level of supported bilayer lipid membranes [19]. Electrochemical impedance spectroscopy (EIS) analysis provides interesting quantitative information about the interphase.…”

Section: Biomimetic Membranes On Electrodesmentioning

confidence: 99%

“…Biomimetic chemistry comes to aid for affronting this challenge. In the last decades, there has been large progress on the formation and characterization of biomimetic lipid bilayers on electrodes for fundamental studies [8,19]. Besides stabilizing the membrane-bound enzyme, the lipid bilayer over the electrode might provide protection from surface fouling or from redox-active interferents.…”

Section: Perspectivesmentioning

confidence: 99%

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Electrochemical Biosensors Based on Membrane-Bound Enzymes in Biomimetic Configurations

Álvarez-Malmagro

García-Molina

Lacey

2020

Sensors

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In nature, many enzymes are attached or inserted into the cell membrane, having hydrophobic subunits or lipid chains for this purpose. Their reconstitution on electrodes maintaining their natural structural characteristics allows for optimizing their electrocatalytic properties and stability. Different biomimetic strategies have been developed for modifying electrodes surfaces to accommodate membrane-bound enzymes, including the formation of self-assembled monolayers of hydrophobic compounds, lipid bilayers, or liposomes deposition. An overview of the different strategies used for the formation of biomimetic membranes, the reconstitution of membrane enzymes on electrodes, and their applications as biosensors is presented.

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Section: Biomimetic Membranes On Electrodesmentioning

confidence: 99%

Section: Biomimetic Membranes On Electrodesmentioning

confidence: 99%

Section: Perspectivesmentioning

confidence: 99%

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Electrochemical Biosensors Based on Membrane-Bound Enzymes in Biomimetic Configurations

Álvarez-Malmagro

García-Molina

Lacey

2020

Sensors

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“…Nowadays lipid membrane constructs can be shaped in many architectures (bilayers, multilayers, mixed layers, branched, doped, nanodiscs, tethered, functionalized, etc.) to serve, efficiently and effectively, a dual purpose: analytical detection [2] and simulation studies [3]. Both research fields benefit from the capability to reproduce some properties of biological membranes and package them into sensing platforms for biosensor devices or membrane interaction studies, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Both research fields benefit from the capability to reproduce some properties of biological membranes and package them into sensing platforms for biosensor devices or membrane interaction studies, respectively. Interestingly, the former field uses the term artificial lipid membranes [2] while the latter exploits similar principles and methods to yield biomembranes or membrane mimics [3], the different connotations refer to the scopes of research and not the research tool per se.…”

Section: Introductionmentioning

confidence: 99%

Recent Lipid Membrane-Based Biosensing Platforms

et al. 2019

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The investigation of lipid films for the construction of biosensors has recently given the opportunity to manufacture devices to selectively detect a wide range of food toxicants, environmental pollutants, and compounds of clinical interest. Biosensor miniaturization using nanotechnological tools has provided novel routes to immobilize various “receptors” within the lipid film. This chapter reviews and exploits platforms in biosensors based on lipid membrane technology that are used in food, environmental, and clinical chemistry to detect various toxicants. Examples of applications are described with an emphasis on novel systems, new sensing techniques, and nanotechnology-based transduction schemes. The compounds that can be monitored are insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc.

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Infrared Reflection–Absorption Spectroscopy

Hollins,

de los Arcos

2024

Encyclopedia of Analytical Chemistry

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Infrared reflection–absorption spectroscopy is an optical technique used to study thin (often submonolayer) films adsorbed on reflective substrates. Experimentally, it involves measuring the change in the reflectance spectrum of the substrate that accompanies adsorption. On metal surfaces, the reflection is usually performed at grazing incidence in order to maximize sensitivity, and the process is subject to an overriding selection rule which states that only those vibrational modes that have a component of their dipole change perpendicular to the surface can be detected. On semiconductors, or dielectric substrates, selection rules depend strongly on the optical characteristics of the surface: vibrational modes with components vibrating in both the perpendicular and the parallel direction to the substrate can be detected, leading to absorption bands whose shape depends strongly on the experimental parameters (incidence angle, light polarization state, angle of the vibration‐induced dipole, and the nature of the substrate). Application of these surface‐induced selection rules often yields important information about adsorption geometry. Other advantages of the technique include its high sensitivity, down to the monolayer, its ability to operate at high ambient pressures or even in liquids, and the ease with which its results can be correlated with those from other vibrational spectroscopies, both surface and bulk sensitive.

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Electrode-supported biomimetic membranes: An electrochemical and surface science approach for characterizing biological cell membranes

Cited by 35 publications

References 162 publications

Electrochemical Biosensors Based on Membrane-Bound Enzymes in Biomimetic Configurations

Electrochemical Biosensors Based on Membrane-Bound Enzymes in Biomimetic Configurations

Recent Lipid Membrane-Based Biosensing Platforms

Infrared Reflection–Absorption Spectroscopy

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