Electrode potential regulates phenol degradation pathways in oxygen-diffused microbial electrochemical system

Zhou, Lean; Yan, Xuejun; Yan, Yuqing; Li, Tian; An, Jingkun; Liao, Chengmei; Li, Nan; Wang, Xin

doi:10.1016/j.cej.2019.122663

Cited by 49 publications

(20 citation statements)

References 49 publications

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“…Customized large BESs (L-BESs, 100 mL in volume) were used in some tests to detect the electroactivity of MFC planktonic cells removed from the biofilms. These BESs contained a graphite rod working electrode, a plain platinum sheet counter electrode, and an Ag/AgCl reference electrode, as previously described …”

Section: Materials and Methodsmentioning

confidence: 99%

“…MFCs were inoculated with domestic wastewater collected from the wastewater treatment plant in the Jinnan campus of Nankai University. The medium used for MFC tests was 1 g/L sodium acetate in a 50 mM phosphate buffer solution (PBS: Na 2 HPO 4 , 4.58 g/L; NaH 2 PO 4 , 2.13 g/L; NH 4 Cl, 0.31 g/L; KCl, 0.13 g/L; trace minerals, 12.5 mL/L; vitamin solution, 5 mL/L) . The medium was refreshed when the cell voltage decreased to <20 mV, forming a cycle that typically had a duration of 2 days .…”

Section: Materials and Methodsmentioning

confidence: 99%

“…These BESs contained a graphite rod working electrode, a plain platinum sheet counter electrode, and an Ag/AgCl reference electrode, as previously described. 34 Single-chambered, three-electrode BESs (15 mL in volume) constructed with serum bottles were used to examine the competition of two Geobacter spp. under oxygen-free conditions.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Long-Term Succession Shows Interspecies Competition of Geobacter in Exoelectrogenic Biofilms

Yan

et al. 2021

Environ. Sci. Technol.

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Geobacter spp. are well-known exoelectrogenic microorganisms that often predominate acetate-fed biofilms in microbial fuel cells (MFCs) and other bioelectrochemical systems (BESs). By using an amplicon sequence variance analysis (at one nucleotide resolution), we observed a succession between two closely related species (98% similarity in 16S RNA), Geobacter sulfurreducens and Geobacter anodireducens, in the long-term studies (20 months) of MFC biofilms. Geobacter spp. predominated in the near-electrode portion of the biofilm, while the outer layer contained an abundance of aerobes, which may have helped to consume oxygen but reduced the relative abundance of Geobacter. Removal of the outer aerobes by norspermidine washing of biofilms revealed a transition from G. sulfurreducens to G. anodireducens. This succession was also found to occur rapidly in cocultures in BES tests even in the absence of oxygen, suggesting that oxygen was not a critical factor. G. sulfurreducens likely dominated in early biofilms by its relatively larger cell size and production of extracellular polymeric substances (individual advantages), while G. anodireducens later predominated due to greater cell numbers (quantitative advantage). Our findings revealed the interspecies competition in the long-term evolution of Geobacter genus, providing microscopic insights into Geobacter's niche and competitiveness in complex electroactive microbial consortia.

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Section: Materials and Methodsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Long-Term Succession Shows Interspecies Competition of Geobacter in Exoelectrogenic Biofilms

Yan

et al. 2021

Environ. Sci. Technol.

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“…The effects of constant electric fields on phenol biodegradation were studied by Ailijiang et al [82], Dehghani et al [83], Zhou et al [84] and Beschkov et al [85]. It was found in [85] that there is an optimum anode potential at which the specific bacterial growth rate for the strain Pseudomonas putida and its biodegrading ability are higher at anode potential of 0.8 V/S.H.E.…”

Section: Phenol Biodegradationmentioning

confidence: 99%

Bioelectrochemical Processes in Industrial Biotechnology

Beschkov¹,

Razkazova-Velkova²

2021

Energy Storage Battery Systems - Fundamentals and Applications

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Industrial fermentation and biological wastewater treatment are usually based on redox processes taking place in living cells and on enzyme processes. The practical application of these redox processes is usually associated with electricity generation in microbial fuel cells or process enhancement in microbial electrolysis cells. The microbial fuel cell approach leads to straightforward wastewater treatment with less energy demand. Additional advantages of these processes are the direct removal of various pollutants and the avoidance of addition of chemical agents with the resulting waste products of treatment as it is familiar with the traditional chemical methods. Another option for the use of bioelectrochemical processes in practice is the approach of microbial electrolysis cells. The application of electric field on fermentation or microbial wastewater treatment processes might result in different aspects: either in purely electrochemical processes on the electrodes or in different types of bioelectrochemical stimulation of enzyme activity in the living cells. These applications are associated with the combination of enzyme activity with electrochemical processes to produce or remove certain compounds rapidly at high concentrations with no additions of other chemicals. In the present chapter, both approaches (microbial fuel cells and microbial electrolysis cells) are presented and discussed. Some practical applications and experimental examples of such bioelectrochemical redox processes stimulated by constant electric field are demonstrated.

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“…Therefore, it is expected that electrochemical reactions on the electrode might enhance the complete microbial biodegradation of the substrate. The effects of constant electric fields on microbial activity were studied for different redox systems-namely, biodegradation of organic matter by Jobin and Namour [17] and Liu et al [18], including phenol biodegradation [19,20].…”

Section: Introductionmentioning

confidence: 99%

Effects of Constant Electric Field on Biodegradation of Phenol by Free and Immobilized Cells of Bradyrhizobium japonicum 273

et al. 2021

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It is shown that bacteria Bradyrhizobium japonicum 273 were capable of degrading phenol at moderate concentrations either in a free cell culture or by immobilized cells on granulated activated carbon particles. The amount of degraded phenol was greater in an immobilized cell preparation than in a free culture. The application of a constant electric field during cultivation led to enhanced phenol biodegradation in a free culture and in immobilized cells on granulated activated carbon. The highest phenol removal efficiency was observed for an anode potential of 1.0 V/S.H.E. The effect was better pronounced in a free culture. The enzyme activities of free cells for phenol oxidation and benzene ring cleavage were very sensitive to the anode potential in the first two steps of the metabolic pathway of phenol biodegradation catalyzed by phenol hydroxylase—catechol-1,2-dioxygenase and catechol-2,3-dioxygenase. It was observed that at an anode potential of 0.8 V/S.H.E., the meta-pathway of cleavage of the benzene ring catalyzed by catechol-2,3-dioxygenase became competitive with the ortho-pathway, catalyzed by catechol-1,2-dioxygenase. The obtained results showed that the positive effect of constant electric field on phenol biodegradation was rather due to electric stimulation of enzyme activity than electrochemical anode oxidation.

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Electrode potential regulates phenol degradation pathways in oxygen-diffused microbial electrochemical system

Cited by 49 publications

References 49 publications

Long-Term Succession Shows Interspecies Competition of Geobacter in Exoelectrogenic Biofilms

Long-Term Succession Shows Interspecies Competition of Geobacter in Exoelectrogenic Biofilms

Bioelectrochemical Processes in Industrial Biotechnology

Effects of Constant Electric Field on Biodegradation of Phenol by Free and Immobilized Cells of Bradyrhizobium japonicum 273

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