Electrochromic behaviour of carotenoid molecules in nerve cell membranes: A resonance Raman study

Paschenko, V.Z.; Vershinin, Alexander; Чурин, А. А.

doi:10.1016/1011-1344(93)80054-d

Cited by 6 publications

(1 citation statement)

References 12 publications

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“…6 Raman spectroscopy has been explored for biomedical applications because it can provide detailed information on the chemical composition of cells and tissues. 7 Raman spectroscopy could be used in the analysis of biological processes within living cells, such as cell biochemical analysis, 8 cell membranes, 9 and chromosomes. 10 And Raman spectroscopy could also be used in the analysis of tissue, such as bone quality, 11 human aorta, 12 mammalian tissue, 13 and others.…”

Section: Introductionmentioning

confidence: 99%

Distinguishing Cancerous Liver Cells Using Surface-Enhanced Raman Spectroscopy

Huang

Liu

Chen

et al. 2014

Technol Cancer Res Treat

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Raman spectroscopy has been widely used in biomedical research and clinical diagnostics. It possesses great potential for the analysis of biochemical processes in cell studies. In this article, the surface-enhanced Raman spectroscopy (SERS) of normal and cancerous liver cells incubated with SERS active substrates (gold nanoparticle) was measured using confocal Raman microspectroscopy technology. The chemical components of the cells were analyzed through statistical methods for the SERS spectrum. Both the relative intensity ratio and principal component analysis (PCA) were used for distinguishing the normal liver cells (QSG-7701) from the hepatoma cells (SMMC-7721). The relative intensity ratio of the Raman spectra peaks such as I 937 /I 1209 , I 1276 /I 1308 , I 1342 /I 1375 , and I 1402 /I 1435 was set as the judge boundary, and the sensitivity and the specificity using PCA method were calculated. The results indicated that the surface-enhanced Raman spectrum could provide the chemical information for distinguishing the normal cells from the cancerous liver cells and demonstrated that SERS technology possessed the possible applied potential for the diagnosis of liver cancer.

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Section: Introductionmentioning

confidence: 99%

Distinguishing Cancerous Liver Cells Using Surface-Enhanced Raman Spectroscopy

Huang

Liu

Chen

et al. 2014

Technol Cancer Res Treat

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Absolute spectroscopic determination of cross-membrane potential

Ehrenberg

Loew

1993

J Fluoresc

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Spectroscopic determination of the cross-membrane electric potential has been used for more than 20 years. This method, which usually employs absorption or fluorescence measurements, allows for a rapid and noninvasive study of the electrical properties of the membranes of cells and liposomes. However, the usual fluorescence techniques preferably allow monitoring changes in the potential on triggerable or excitable membranes, and not the absolute value of the potential. They also do not provide means for measuring the potential on single cells. This paper reviews three methods that solve these issues. Nernstian dyes which partition between intra-and extracompartmental volumes enable a fluorescence microscopic determination of a single cell and even a single organelle. Dual-wavelength ratiometric recording from membrane-staining dyes also provides means for measuring the field on a single cell. Resonance Raman probes provide a spectroscopic method with a natural internal standard for the absolute measurement of membrane potential.

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Carotenoids in mollusca: approaching the functions

Vershinin

1996

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Electrochromic behaviour of carotenoid molecules in nerve cell membranes: A resonance Raman study

Cited by 6 publications

References 12 publications

Distinguishing Cancerous Liver Cells Using Surface-Enhanced Raman Spectroscopy

Distinguishing Cancerous Liver Cells Using Surface-Enhanced Raman Spectroscopy

Absolute spectroscopic determination of cross-membrane potential

Carotenoids in mollusca: approaching the functions

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