Electrochemistry of mercury supported phospholipid monolayers and bilayers

Nelson, Andrew

doi:10.1016/j.cocis.2010.07.004

Cited by 45 publications

(56 citation statements)

References 63 publications

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“…The R and C values for all RC meshes of the four lipid SAMs are reported in the legends for the corresponding figures; the values for the two RC meshes ascribable to the hydrocarbon tails and the polar heads of the monolayers of DOPC, DCP, DOPC/GM1 and DCP/GM1 are plotted in Figure 5, in the above order, to evidence possible trends. The capacitance of the hydrocarbon tails of the DOPC SAM, C ht = 1.9 µF·cm −2 , is about twice that, between 0.9 and 1 µF·cm −2 , of solvent-free bilayer lipid membranes and in good agreement with the value of 1.80 µF·cm −2 , reported by Nelson [21]. Addition of 10 nM ChTo B to the aqueous solution bathing the lipid monolayers that incorporate GM1 has a negligible effect on their impedance spectra, even after an incubation time of 1 h. Figure 1.…”

Section: Resultssupporting

confidence: 76%

“…A homemade hanging mercury drop electrode (HMDE) was then immersed into the aqueous solution across the lipid film using an oleo-dynamic system. This procedure gives rise to a lipid monolayer with the hydrocarbon tails directed toward the mercury surface, thanks to its hydrophobic nature [20][21][22]. This lipid self-assembly is equivalent to a Langmuir-Blodgett transfer at the equilibrium spreading pressure [23].…”

Section: Preparation Of Mercury-supported Self-assembled Monolayers (mentioning

confidence: 99%

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The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts

et al. 2014

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Mercury-supported, self-assembled monolayers (SAMs) of the sole dioleoylphosphatidylcholine (DOPC) and of a raft-forming mixture of DOPC, cholesterol (Chol) and palmitoylsphingomyelin (PSM) of (59:26:15) mol% composition, were investigated by electrochemical impedance spectroscopy (EIS), both in the absence and in the presence of the monosialoganglioside GM1. The impedance spectra of these four SAMs were fitted by a series of parallel combinations of a resistance and a capacitance (RC meshes) and displayed on plots of ωZ′ against −ωZ″, where Z′ and Z″ are the in-phase and quadrature components of the impedance and ω is the angular frequency. A comparison among these different impedance spectra points to the formation of GM1-rich gel phase microdomains within the lipid rafts of the DOPC/Chol/PSM mixture, thanks to the unique molecular-level smooth support provided by mercury, which allows EIS to detect the protruding gel phase microdomains by averaging them over a macroscopically large area.

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Section: Resultssupporting

confidence: 76%

Section: Preparation Of Mercury-supported Self-assembled Monolayers (mentioning

confidence: 99%

The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts

et al. 2014

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“…These systems can be used, e.g., as tools for the study of the properties of lipid membranes and how they are affected by the surrounding conditions (e.g., electric potential 1,2 , composition of the bulk solution 3 etc.). They are also of great interest as systems to study membrane-analyte interactions, including the study of peptide-and protein-membrane interactions 4,5 .…”

Section: Introductionmentioning

confidence: 99%

Label-Free Characterization of Peptide–Lipid Interactions Using Immobilized Lipodisks

2013

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“…For the study of quinone incorporation in biomimetic membranes, both the monolayer formation and the unilamellar vesicle formation [6] approaches can be used. One of the first attempts to study the electrochemistry of UQ used lipid monolayer and bilayer systems supported on Hg [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Electrochemistry of LB films of mixed MGDG:UQ on ITO

Hoyo

Guaus

Torrent‐Burgués

et al. 2015

Bioelectrochemistry

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. C/ Colom 1, 08222 Terrassa (Barcelona), Spain. Tl: 34 937398043. AbstractThe electrochemical behaviour of biomimetic monolayers of monogalactosyldiacylglycerol (MGDG) incorporating ubiquinone-10 (UQ) has been investigated. MGDG is the principal component in the thylakoid membrane and UQ seems a good substitute for plastoquinone-9, involved in photosynthesis chain. The monolayers have been performed using the Langmuir and Langmuir-Blodgett (LB) techniques and the redox behaviour of the LB films, transferred at several surface pressures on a glass covered with indium-tin oxide (ITO), has been characterized by cyclic voltammetry. The cyclic voltammograms show that UQ molecules present two redox processes (I and II) at high UQ content and high surface pressures, and only one redox process (I) at low UQ content and low surface pressures. The apparent rate constants calculated for processes I and II indicate a different kinetic control for the reduction and the oxidation of UQ/UQH2 redox couple, being kRapp(I)= 2.2·10 -5 s , respectively. The correlation of the redox response with the physical states of the LB films allows determining the positions of the UQ molecules in the biomimetic monolayer, which change with the surface pressure and the UQ content. These positons are known as diving and swimming.

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Electrochemistry of mercury supported phospholipid monolayers and bilayers

Cited by 45 publications

References 63 publications

The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts

The GM1 Ganglioside Forms GM1-Rich Gel Phase Microdomains within Lipid Rafts

Label-Free Characterization of Peptide–Lipid Interactions Using Immobilized Lipodisks

Electrochemistry of LB films of mixed MGDG:UQ on ITO

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