Electrochemistry of cast films of ferredoxin and lipid bilayers on electrodes

Hydrogen Peroxide Biosensor Based on the Electrochemistry of the Myoglobin‐TATP Composite Film

“…Among various redox proteins, heme proteins are mostly studied. Many materials have been used to incorporate heme proteins, including insoluble surfactant (Mimica et al 2001), hydrogel polymer (Å sberg andInganäs 2003;Liu et al 2004), biopolymer (Nassar et al 1996), nanoparticles or clay nanoparticles (Li and Hu 2003), and polyelectrolyte-or clay composite (Liu and Gooding 2006). Heme proteins in these films have been characterized to enhance the direct, quasi-reversible electron transfer on the electrode surface compared with those in solutions.…”

Electrochemical Determination of Serotonin and the Competitive Adsorption with Dopamine at 5,5-Ditetradecyl-2-(2-trimethyl-ammonioethyl)-1,3-dioxane Bromide Lipid Film Modified by Glassy Carbon Electrode

“…The surface concentration exhibits the 32-fold enrichment effect from 1.5 × 10 -5 M 5-HT solution. Compared with the result of DA exhibiting the 5000-fold enrichment effect from 1.25 × 10 -4 M DA solution, 27 obviously, DTDB-BLM shows a higher affinity for DA than 5-HT. It has to be noted that the enrichment effect is a vital factor to obtain the highly sensitive determination of neurotransmitter at DTDB/GCE.…”

“…22 As a biocompatible environment, BLM has been doped with some substance such as nanoparticles, 23 dentritic polymer 24 and bioactive molecules to study the complicated function of membrane. [25][26][27][28] Herein, we studied the electrochemical behavior of 5-HT at a synthetic lipid of 5,5-ditetradecyl-2-(2-trimethylammonioethyl)-1,3-dioxane bromide (DTDB) selfassembled BLM modified glassy carbon electrode (DTDB/GCE). In our previous work, 29 a strong electrocatalytic effect for dopamine (DA) at DTDB/GCE has ever been reported.…”

Electroactive Films of Alternately Layered Polycations and Iron–Sulfur Protein Putidaredoxin on Gold

“…In contrast, films of surfactants or polyions containing heme proteins were stable for several weeks to a month when stored in cold buffer (10,11). Part of the problem with Pdx may be its inherent instability, but other small ferredoxin proteins were also stable for only a day in surfactant films (44). While Pdx/PDDA film stability was sufficient for characterization, additional stability might eventually be achieved with these small proteins by considering layered covalent-bonding strategies (45).…”