Electrochemiluminescent determination of the activity of uracil-DNA glycosylase: Combining nicking enzyme assisted signal amplification and catalyzed hairpin assembly

A Methodology for Ultrasensitive Detection of Sequence-Specific DNA or Uracil-DNA Glycosylase Activity

“…Maintaining the integrity of the genome is the presupposition for organisms to accomplish gene transcription . Regrettably, the stabilization and veracity of the genome can be disturbed by some endogenous or exogenous factors, such as radiation, genetic toxic chemicals, and UV irradiation. , For example, the deamination of cytosine may lead to the construction of U:G in double-stranded DNA, eventually causing the transformation of G:C to A:T . Thus, it is of considerable importance for organisms to repair such DNA lesions.…”