Electrochemiluminescence on digital microfluidics for microRNA analysis

Shamsi, Mohtashim H.; Choi, Kihwan; Ng, Alphonsus H. C.; Chamberlain, M. Dean; Wheeler, Aaron R.

doi:10.1016/j.bios.2015.10.036

Cited by 69 publications

(42 citation statements)

References 63 publications

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“…21 "Open"-format microfluidic systems such as those powered by digital microfluidics (DMF) eliminate the problem of clogging and have proven particularly useful for handling magnetic micro-particles 22 for the analysis of small molecules, 23,24 proteins, [25][26][27][28][29][30][31] and nucleic acids. 32,33 In the most common DMF device format, discrete droplets of liquid are sandwiched between two plates: the bottom plate comprises an array of electrodes that is covered by an insulating dielectric layer and a hydrophobic layer, and the top plate comprises a ground electrode that is covered with a hydrophobic layer. Droplets are moved by sequential application of voltages to electrodes adjacent to the droplet.…”

Section: Introductionmentioning

confidence: 99%

“…In this two-plate format, droplets cannot be manipulated in the z-axis but are unrestricted by any walls or barriers in the xy-plane. 34 Despite its proven utility in handling magnetic microparticles, [22][23][24][25][26][27][28][29][30][31][32][33] DMF is limited in its ability to effect preconcentrations of significant magnitude. Specifically, the difference between the smallest and largest volumes that can be (practically) manipulated on most DMF devices is often quite smalle.g., in the devices used here, this range runs from ∼0.9 μL (a "unit" droplet covering one electrode) to ∼9 μL (a droplet covering 10 electrodes; volumes larger than this are impractical to use).…”

Section: Introductionmentioning

confidence: 99%

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Pre-concentration by liquid intake by paper (P-CLIP): a new technique for large volumes and digital microfluidics

et al. 2017

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Microfluidic platforms are an attractive option for incorporating complex fluid handling into low-cost and rapid diagnostic tests. A persistent challenge for microfluidics, however, is the mismatch in the "world-to-chip" interface - it is challenging to detect analytes present at low concentrations in systems that can only handle small volumes of sample. Here we describe a new technique termed pre-concentration by liquid intake by paper (P-CLIP) that addresses this mismatch, allowing digital microfluidics to interface with volumes on the order of hundreds of microliters. In P-CLIP, a virtual microchannel is generated to pass a large volume through the device; analytes captured on magnetic particles can be isolated and then resuspended into smaller volumes for further processing and analysis. We characterize this method and demonstrate its utility with an immunoassay for Plasmodium falciparum lactate dehydrogenase, a malaria biomarker, and propose that the P-CLIP strategy may be useful for a wide range of applications that are currently limited by low-abundance analytes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pre-concentration by liquid intake by paper (P-CLIP): a new technique for large volumes and digital microfluidics

et al. 2017

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“…An alternative to standard microfluidic ECL designs was described by Shamsi et al [68] They reported the first integration of ECL detection with a mode of microfluidics known as digital microfluidics (DMF). DMF is a liquid handling platform that is used to move, dispense, mix, and split droplets on an array of insulated electrodes.…”

Section: Other Novel Devicesmentioning

confidence: 99%

Electrochemiluminescence Detection in Paper‐Based and Other Inexpensive Microfluidic Devices

et al. 2017

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There is a need in the field of microfluidics for integration of analytical detection methods onto small fluidic chips. Electrogenerated chemiluminescence (ECL) is an effective method for detecting a wide range of analytes, including small molecules, metal ions and bacteria. This Minireview discusses recent applications of ECL-based detection methods to inexpensive microfluidic devices. We discuss various paper and cloth based devices, including 3D-origami devices and devices utilizing bipolar electrodes. We also discuss novel devices that have replaced traditional instrumentation with inexpensive and portable equipment, such as mobile phones.

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“…13,14 This attribute enables reliable fluid handling, preparation, and analysis of biological samples. Further, digital microfluidic devices have been used for polymerase chain reactions (PCR), 15,16 DNA sequencing, 17 assays for clinical diagnosis 18 (cell assays, 19 enzyme assays, [20][21][22] and immunoassays [23][24][25] ), SPRi detection of DNA hybridization, 26 micro RNA analysis, 27 cell studies, 28 protein analysis, 29 and chemical separation. [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] Our co-authors first presented on the use of a commercial platform (Advanced Liquid Logic, Inc.) to perform successful electrotransformation of E. coli on an EWoD digital microfluidic platform 10 and characterized the impact of integrated electroporation devices to fluid transport in the EWoD format.…”

Section: Introductionmentioning

confidence: 99%

Automated electrotransformation of Escherichia coli on a digital microfluidic platform using bioactivated magnetic beads

Moore¹,

Nemat‐Gorgani²,

Madison

et al. 2017

Biomicrofluidics

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This paper reports on the use of a digital microfluidic platform to perform multiplex automated genetic engineering (MAGE) cycles on droplets containing Escherichia coli cells. Bioactivated magnetic beads were employed for cell binding, washing, and media exchange in the preparation of electrocompetent cells in the electrowetting-on-dieletric (EWoD) platform. On-cartridge electroporation was used to deliver oligonucleotides into the cells. In addition to the optimization of a magnetic bead-based benchtop protocol for generating and transforming electrocompetent E. coli cells, we report on the implementation of this protocol in a fully automated digital microfluidic platform. Bead-based media exchange and electroporation pulse conditions were optimized on benchtop for transformation frequency to provide initial parameters for microfluidic device trials. Benchtop experiments comparing electrotransformation of free and beadbound cells are presented. Our results suggest that dielectric shielding intrinsic to bead-bound cells significantly reduces electroporation field exposure efficiency. However, high transformation frequency can be maintained in the presence of magnetic beads through the application of more intense electroporation pulses. As a proof of concept, MAGE cycles were successfully performed on a commercial EWoD cartridge using variations of the optimal magnetic bead-based preparation procedure and pulse conditions determined by the benchtop results. Transformation frequencies up to 22% were achieved on benchtop; this frequency was matched within 1% (21%) by MAGE cycles on the microfluidic device. However, typical frequencies on the device remain lower, averaging 9% with a standard deviation of 9%. The presented results demonstrate the potential of digital microfluidics to perform complex and automated genetic engineering protocols. Published by AIP Publishing. [http://dx

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Electrochemiluminescence on digital microfluidics for microRNA analysis

Cited by 69 publications

References 63 publications

Pre-concentration by liquid intake by paper (P-CLIP): a new technique for large volumes and digital microfluidics

Pre-concentration by liquid intake by paper (P-CLIP): a new technique for large volumes and digital microfluidics

Electrochemiluminescence Detection in Paper‐Based and Other Inexpensive Microfluidic Devices

Automated electrotransformation of Escherichia coli on a digital microfluidic platform using bioactivated magnetic beads

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