Electrochemiluminescence nanoimmunosensor for CD63 protein using a carbon nanochips/iron oxide/nafion-nanocomposite modified mesoporous carbon interface

Adhikari, Juthi; Rizwan, Mohammad; Dennany, Lynn; Ahmed, Minhaz Uddin

doi:10.1016/j.measurement.2020.108755

Cited by 10 publications

(8 citation statements)

References 47 publications

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“…44 Moreover, the proposed biosensor was experimentally demonstrated to detect the lowest concentration 1 pgmL -1 of gelatin. 23,34 This low limit of detection of the immunosensor could be due to the synergistic electrochemical conductive properties provided both by CNFs and CNHs. 21,22 Fig.…”

Section: Analytical Performance Of the Biosensormentioning

confidence: 99%

“…This established that developed CNFs-SPE/CNHs/NAF/anti-gltn/BSA biosensor follow the electrochemically diffusion control kinetics for the detection of porcine gelatin. 23,34 Fig. 8: Diffusion kinetic study of biosensor assessed by ECL intensity (A) ECL intensity of the developed immunosensor from 20 mVs -1 to 100 mVs -1 at an interval of 20; and (B) relationship between the peak value of ECL intensity and square root of scan rates.…”

Section: Label-free Detection Principlementioning

confidence: 99%

“…Therefore, a medium of 10 mM PBS having optimum ionic strength for sensing application has been used following standard references. 23,34 Moreover, to confirm the optimum pH for the ECL signal of the biosensor, different pH ranges (5.4, 6.4, 7.4, and 8.4) were tested. The biosensor demonstrated the highest and stable ECL signal with 10 mM PBS solution of pH 7.4 (Fig.…”

Section: Ph Evaluation Of the Reaction Mediummentioning

confidence: 99%

“…This could be attributed to the insulating nature of porcine gelatin that inhibits electron transfer between the ECL probe and the working electrode surface. 34,42 Thus, up to saturation concentration (8 ng mL −1 ), the biosensor responded to bind porcine gelatin, and correspondingly the ECL intensity decreased. 43 Further, this evaluated concentration range is acceptable for detecting gelatin in food.…”

Section: Analytical Performance Of the Biosensormentioning

confidence: 99%

“…44 Moreover, the proposed biosensor was experimentally demonstrated to detect the lowest concentration of 1 pg mL −1 of gelatin. 23,34 This low limit of detection of the immunosensor could be due to the synergistic electrochemical conductive properties provided by both CNFs and CNHs. 21,22 3.7 Label-free detection principle ECL scan rate study of the CNFs-SPE/CNHs/NAF/anti-gltn/BSA biosensor incubated with gelatin was performed to evaluate the electrochemical diffusion control process.…”

Section: Analytical Performance Of the Biosensormentioning

confidence: 99%

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Development of a label-free electrochemiluminescence biosensor for the sensitive detection of porcine gelatin using carbon nanostructured materials

2022

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Section: Analytical Performance Of the Biosensormentioning

confidence: 99%

Section: Label-free Detection Principlementioning

confidence: 99%

Section: Ph Evaluation Of the Reaction Mediummentioning

confidence: 99%

Section: Analytical Performance Of the Biosensormentioning

confidence: 99%

Section: Analytical Performance Of the Biosensormentioning

confidence: 99%

See 3 more Smart Citations

Development of a label-free electrochemiluminescence biosensor for the sensitive detection of porcine gelatin using carbon nanostructured materials

2022

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Harnessing the Therapeutic Potential of Extracellular Vesicles for Biomedical Applications Using Multifunctional Magnetic Nanomaterials

Yang

Patel

Rathnam

et al. 2022

Small

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muscle cells, and stem cells. [5][6][7][8][9][10][11][12] Although initially considered as membrane debris with no biological significance, the crucial roles of EVs in immune surveillance, viral infection, blood coagulation, tissue repair, and stem cell maintenance, have been sequentially identified since 1996. [6,[13][14][15][16][17][18][19] Additionally, biomolecular compositions inside EVs have also been closely associated with the pathology of various kinds of diseases such as cancers, musculoskeletal diseases, as well as degenerative neurological disorders. [20][21][22] As a result of the fundamental role of EVs in mediating cell-to-cell communications and regulating various tissue functions, there has been a critical need to develop novel therapeutics and diagnostics (or theranostics) using EVs and their associated biomolecules. [23] For example, cellfree therapy using EVs derived from mesenchymal stem cells (MSCs) has been exploited and is currently under clinical trials to enhance tissue regeneration after cardiac infarction. [6,7,17,18] Similarly, EVs capable of crossing the bloodbrain barrier (BBB) have been utilized for intranasal delivery of therapeutics for treating central nervous system (CNS) injuries. [24][25][26][27] Moreover, minimally invasive, highly sensitive/accurate diagnosis of cancer metastasis, viral infection, Prion diseases, Alzheimer's disease (AD), and Parkinson's disease (PD), has also been realized by analyzing the biomolecular formulation of EVs extracted from human body fluids. [28,29] Although the potential of using EVs for theranostic applications is enormous, their current clinical translation is still impeded by several critical barriers, which can be attributed mainly to the high heterogeneity of EVs. In general, there are four levels of heterogeneity of EVs, including size, composition, function, and source heterogeneities. [2,30] Specifically, most clinical applications often require a well-defined source of therapeutics. Sizes, compositions, functions, and sources have all been closely associated with the outcome of clinical treatment and diagnostic diseases. [30][31][32][33][34][35][36] Having the ability to isolate a specific population of EVs with well-defined sizes, biomolecular contents, disease-specific biological functions, and biodistributions would be crucial for the clinical application of EVs. Failing to achieve this can lead to compromised therapeutic effects and sometimes even adverse outcomes. For example, the multifaceted roles of EVs have been identified for regulating Extracellular vesicles (e.g., exosomes) carrying various biomolecules (e.g., proteins, lipids, and nucleic acids) have rapidly emerged as promising platforms for many biomedical applications. Despite their enormous potential, their heterogeneity in surfaces and sizes, the high complexity of cargo biomolecules, and the inefficient uptake by recipient cells remain critical barriers for their theranostic applications. To address these critical issues, multifunctional nanomaterials, such as magn...

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Flexible electrochemical paper-based device for detection of breast cancer-derived exosome using nickel nanofoam 3D nanocomposite

Sahraei,

Mazloum-Ardakani,

Moradi

et al. 2024

J Appl Electrochem

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Exosomes as new biomarkers for cancer diagnosis have attracted attention because they are highly released by tumor cells in various biological uids. In this study, an electrochemical paper-based immunosensor device (Exo-sensing paper) is introduced for the detection of exosome in the serum. The Exo-sensing paper is a three electrode system that is prepared using pattern paper and carbon and silver inks. The sensor part of this immunosensor contains a three-dimensional porous nanocomposite of nickel nanofoam coupled with graphene oxide and gold nanoparticles. The high speci c surface area of this nanocomposite increases the antibody loading on the sensor surface signi cantly and consequently leads to obtaining a wide linear range of 500-1 × 10 7 Exospore/µL with a detection limit of 110 Exosome/µL. Due to some advantages of this constructed Exo-sensing paper such as easy storage, simple application, low cost and good selectivity in the real samples, this system has a good potential to be used as a point of care testing for in situ detection of the exosomes and is a promising strategy for minimally invasive liquid biopsy.

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Electrochemiluminescence nanoimmunosensor for CD63 protein using a carbon nanochips/iron oxide/nafion-nanocomposite modified mesoporous carbon interface

Cited by 10 publications

References 47 publications

Development of a label-free electrochemiluminescence biosensor for the sensitive detection of porcine gelatin using carbon nanostructured materials

Development of a label-free electrochemiluminescence biosensor for the sensitive detection of porcine gelatin using carbon nanostructured materials

Harnessing the Therapeutic Potential of Extracellular Vesicles for Biomedical Applications Using Multifunctional Magnetic Nanomaterials

Flexible electrochemical paper-based device for detection of breast cancer-derived exosome using nickel nanofoam 3D nanocomposite

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