Electrochemiluminescence immunosensor for α-fetoprotein using Ru(bpy)32+-encapsulated liposome as labels

Wang, Haiyan; Sun, Dongyan; Tan, Zhijun; Gong, Wei; Wang, Lun

doi:10.1016/j.colsurfb.2011.02.008

Cited by 30 publications

(5 citation statements)

References 19 publications

(29 reference statements)

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“…DpAu with large specific area and high electrical conductivity could improve the conductivity of the electrode, thus after DpAu were deposited onto Au electrode, the charge transfer resistance (Rct) decreased (curve b), demonstrating that DpAu have been successfully assembled on the bare Au electrode (curve a), which is consitent with previous reports [24]. When PA was immobilized on the electrode surface, Rct obviously increased (curve c), which was ascribed the inhibition effect of PA biomacromolecules for electron transfer [21].…”

Section: Resultssupporting

confidence: 88%

Amperometric Immunosensor Based on a Protein A/Deposited Gold Nanocrystals Modified Electrode for Carbofuran Detection

Sun

Zhu

Wang

2011

Sensors

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In this paper, an amperometric immunosensor modified with protein A/deposited gold nanocrystals (DpAu) was developed for the ultrasensitive detection of carbofuran residues. First, DpAu were electrodeposited onto the Au electrode surface to absorb protein A (PA) and improve the electrode conductivity. Then PA was dropped onto the surface of DpAu film, used for binding antibody Fc fragments. Next, anti-carbofuran monoclonal antibody was immobilized on the PA modified electrode. Finally, bovine serum albumin (BSA) was employed to block the possible remaining active sites avoiding any nonspecific adsorption. The fabrication procedure of the immunosensor was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), respectively. With the excellent electroconductivity of DpAu and the PA’s oriented immobilization of antibodies, a highly efficient immuno-reaction and detection sensitivity could be achieved. The influences of the electrodeposition time of DpAu, pH of the detection solution and incubation time on the current response of the fabricated immunosensor were investigated. Under optimized conditions, the current response was proportional to the concentration of carbofuran which ranged from 1 to 100 ng/mL and 100 ng/mL to 100 μg/mL. The detection limit was 0.1924 ng/mL. The proposed carbofuran immnuosensor exhibited high specificity, reproducibility, stability and regeneration performance, which may open a new door for ultrasensitive detection of carbofuran residues in vegetables and fruits.

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Section: Resultssupporting

confidence: 88%

Amperometric Immunosensor Based on a Protein A/Deposited Gold Nanocrystals Modified Electrode for Carbofuran Detection

Sun

Zhu

Wang

2011

Sensors

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“…S3). AuNP film electrode provides high surface area, high adsorptivity and signal amplification of multiple enzyme labels with excellent catalytic capability (Mani et al, 2009;Rusling and Sotzing, 2009;Chikkaveeraiah et al, 2011;Ding et al, 2009;Liang et al, 2009;Yuan et al, 2010;Wang et al, 2011). Gold nanoparticles functionalized with 1,1′-bis-(2-mercapto)-4,4′-bipyridinium dibromide, a kind of sulfhydryl viologen (SV) were coated on the surface of GCE after which capture antibodies were attached.…”

Section: As Carriers In Fabrication Of Electrodesmentioning

confidence: 99%

Nanomaterials for early detection of cancer biomarker with special emphasis on gold nanoparticles in immunoassays/sensors

Devi

Doble

Verma

2015

Biosensors and Bioelectronics

170

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“…Sensitive detection of AFP is crucial for many areas of modern biochemical and biomedical research, providing opportunities for understanding the fundamental biological processes involved in disease progression, and for monitoring the patient response to therapy. Conventional immunoassay methods, including the enzyme-linked immunosorbent assay (ELISA) [ 4 , 5 ], radioimmunoassay [ 6 , 7 ], fluorescence immunoassay [ 8 , 9 ], chemiluminescence assay [ 10 , 11 ], electrophoretic immunoassay [ 12 ], mass spectrometric immunoassay [ 13 , 14 ] and immune-polymerase chain reaction assay [ 15 ], allow for reliable predictions. ELISA is widely used as an immunoassay method in clinical laboratories.…”

Section: Introductionmentioning

confidence: 99%

An Ultrasensitive Electrochemical Immunosensor for Alpha-Fetoprotein Using an Envision Complex-Antibody Copolymer as a Sensitive Label

et al. 2012

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A novel strategy is presented for sensitive detection of alfa-fetoprotein (AFP), using a horseradish peroxidase (HRP)-functionalized Envision antibody complex (EVC) as the label. The Envision-AFP signal antibody copolymer (EVC-AFP Ab2) was composed of a dextran amine skeleton anchoring more than 100 molecules of HRP and 15 molecules of secondary antibody, and acted as a signal tag in the immunosensor. The sensor was constructed using the following steps: First, gold electrode (GE) was modified with nano-gold (AuNPs) by electro-deposition in HAuCl4 solution. The high affinity of the AuNPs surface facilitates direct formation of a self-assembled thiolated protein G layer. Next, the coated GE was incubated in a solution of AFP capture antibody (AFP Ab1); these antibodies attach to the thiolated protein G layer through their non-antigenic regions, leaving the antigen binding sites for binding of target analyte. Following a sandwich immunoreaction, an EVC-AFP Ab2-AFP-AFP Ab1 immunocomplex was formed on the electrode surface, allowing large amounts of HRP on the complex to produce an amplified electrocatalytic current of hydroquinone (HQ) in the presence of hydrogen peroxide (H2O2). Highly amplified detection was achieved, with a detection limit of 2 pg/mL and a linear range of 0.005–0.2 ng/mL for AFP in 10 μL undiluted serum; this is near or below the normal levels of most cancer biomarker proteins in human serum. Measurements of AFP in the serum of cancer patients correlated strongly with standard enzyme-linked immunosorbent assays. These easily fabricated EVC-modified immunosensors show excellent promise for future fabrication of bioelectronic arrays. By varying the target biomolecules, this technique may be easily extended for use with other immunoassays, and thus represents a versatile design route.

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Electrochemiluminescence immunosensor for α-fetoprotein using Ru(bpy)32+-encapsulated liposome as labels

Cited by 30 publications

References 19 publications

Amperometric Immunosensor Based on a Protein A/Deposited Gold Nanocrystals Modified Electrode for Carbofuran Detection

Amperometric Immunosensor Based on a Protein A/Deposited Gold Nanocrystals Modified Electrode for Carbofuran Detection

Nanomaterials for early detection of cancer biomarker with special emphasis on gold nanoparticles in immunoassays/sensors

An Ultrasensitive Electrochemical Immunosensor for Alpha-Fetoprotein Using an Envision Complex-Antibody Copolymer as a Sensitive Label

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