Electrochemiluminescence imaging of a membrane carcinoembryonic antigen at single tissue sections

Shi, Junwei; Han, Dongni; Feng, Zengyu; Jiang, Dechen; Jiang, Depeng

doi:10.1039/d3an00187c

“…Using the established method, membrane carcinoembryonic antigens at single tissue sections of cancer patients were analyzed. [91] In combination with epitope retrieval, the sensitivity of ECL imaging can be further improved. [92] This new molecular imaging approach may help the diagnosis of tumor types and origins.…”

Section: Ecl Microscopy Of Biological Entitiesmentioning

confidence: 99%

Electrochemiluminescence Microscopy

Knežević,

Han,

Liu

et al. 2024

Angewandte Chemie

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Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into an optical microscopy. The orthogonality of the electrochemical trigger and the optical readout distinguishes it from classic microscopy and electrochemical techniques, owing to its near‐zero background, remarkable sensitivity, and absence of photobleaching and phototoxicity. In this review, we summarize the recent advances in ECL imaging technology, emphasising original configurations which enable the imaging of biological entities and the improvement of the analytical properties by increasing the complexity and multiplexing of bioassays. Additionally, mapping the (electro)chemical reactivity in space provides valuable information on nanomaterials and facilitates deciphering ECL mechanisms for improving their performances in diagnostics and (electro)catalysis. Finally, we highlight the recent achievements in imaging at the ultimate limits of single molecules, single photons or single chemical reactions, and the current challenges to translate the ECL imaging advances to other fields such as material science, catalysis and biology.

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Electrochemiluminescence Microscopy

Knežević,

Han,

Liu

et al. 2024

Angew Chem Int Ed

Self Cite

3

0

View full text Add to dashboard Cite

Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into an optical microscopy. The orthogonality of the electrochemical trigger and the optical readout distinguishes it from classic microscopy and electrochemical techniques, owing to its near‐zero background, remarkable sensitivity, and absence of photobleaching and phototoxicity. In this review, we summarize the recent advances in ECL imaging technology, emphasising original configurations which enable the imaging of biological entities and the improvement of the analytical properties by increasing the complexity and multiplexing of bioassays. Additionally, mapping the (electro)chemical reactivity in space provides valuable information on nanomaterials and facilitates deciphering ECL mechanisms for improving their performances in diagnostics and (electro)catalysis. Finally, we highlight the recent achievements in imaging at the ultimate limits of single molecules, single photons or single chemical reactions, and the current challenges to translate the ECL imaging advances to other fields such as material science, catalysis and biology.

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