Electrochemiluminescence Aptasensor for the MUC1 Protein Based on Multi‐functionalized Graphene Oxide Nanocomposite

Abstract

The electrochemiluminescence (ECL) aptasensor was prepared for the detection of Mucin 1 based on its specific recognition by aptamer immobilized on multi‐functionalized graphene oxide nanocomposite, which was prepared with N‐(4‐aminobutyl)‐N‐ethylisoluminol (ABEI) and aptamer chemically bound to the surface of magnetic GO (nanoFe3O4@GO). ABEI and aptamer acted as the electrochemiluminophore and the capture device for Mucin 1 respectively. NanoFe3O4@GO brought multi‐functionalized graphene oxide nanocomposite attracted on the surface of magnetic glass carbon electrode through magnetism, enabled all the ABEI immobilized electrochemically active due to its good conductivity and thus then facilitated the sensitive detection of Mucin 1. In addition, the ECL aptasensor can be prepared through a one‐step process. Under optimal conditions, the ECL intensity of the aptasensor decreased proportionally to the logarithmic concentrations of Mucin 1 in the range of 0.005–1000 ng mL−1. This aptasensor displays good specificity, stability, reproducibility and application. This method has a large potential because such a multi‐functionalized graphene oxide nanocomposite also may be applied to other ECL‐based aptasensors.

Electrochemical, Electrochemiluminescence, and Photoelectrochemical Aptamer-Based Nanostructured Sensors for Biomarker Analysis

“…Examples of electrochemiluminescence aptasensor for platelet-derived growth factor B chain (PDGF-BB) and MUC1 can be found in [ 90 , 91 ], respectively. In both case N -(aminobutyl)- N -ethylisoluminol (ABEI) was used as emitter.…”

Functionalized Thiophene-Based Aptasensors for the Electrochemical Detection of Mucin-1

“…The correlation between MUC1-derived “cancer antigen epitopes” and patient prognosis is therefore a critical element of cancer screening. Indeed, serial CA 15-3 measurements are commonly used to detect early recurrence and monitor metastatic breast cancer patients during follow-up. − Traditional methods such as enzyme-linked immunosorbent assay, dot blotting, western blotting, immunofluorescence, and immunohistochemistry have been utilized for the detection of MUC1, but they are time-consuming, labor-intensive, and require a high level of expertise and dedicated instrumentation, therefore limiting their use in real-time, point-of-care clinical diagnostics. , For widespread adoption of MUC1-based screening of cancer, it is important to develop novel sensors for the rapid, sensitive, and selective detection of MUC1. Electrochemical detection methods have been researched in recent years as a good alternative to more traditional methods of biological detection due to their ease of use, rapid response, and high selectivity and sensitivity. − …”

A Novel Solid‐state Electrochemiluminescent Enantioselective Sensor for Ascorbic Acid and Isoascorbic Acid

“…Electrochemiluminescence (ECL), as a new developing and powerful analytical tool for detection, has been used for chemical and biological analysis based on its distinct advantages of simplicity, rapidity, sensitivity and controllability . Most ECL sensors are used for the detection of biomacromolecules . While, there are few reports of using ECL sensors for chiral discrimination and detection of chiral compounds based on chiral selectors .…”