Electrochemically reduced graphene oxide on CoCr biomedical alloy: Characterization, macrophage biocompatibility and hemocompatibility in rats with graphene and graphene oxide

Escudero, M.L.; Llorente, Irene; Pérez-Maceda, B. T.; José-Pinilla, S.; Sánchez-López, L.; Lozano, Rosa M.; Aguado-Henche, Soledad; Arriba, Celia Clemente de; Alobera-Gracia, Miguel Ángel; García-Alonso, M. C.

doi:10.1016/j.msec.2019.110522

Cited by 28 publications

(19 citation statements)

References 29 publications

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“…In trauma applications, the increase in lubrication in the replaced joints is extremely important. Bearing this in mind and knowing that hyaluronic acid is the main lubricant component of the synovial fluid, this paper deals with the functionalization with HA of ErGO, deposited as a solid lubricant, which is an additional step in the modification and improvement of the CoCr articular surfaces [11,12].…”

Section: Resultsmentioning

confidence: 99%

“…To measure the macrophage biocompatibility of CoCrErGOHA, CoCrErGO, and CoCr discs, a ratio between LDH/WST-1 values was employed to relate plasma membrane damage to the number of metabolically active cells in culture [12].…”

Section: Measurement Of Lactate Dehydrogenase Activitymentioning

confidence: 99%

“…To further confirm the improvement in the viability observed upon functionalization of CoCrErGO with HA, the LDH/WST-1 activity ratio has been measured here for each material as a biocompatibility index. This ratio relates cell death to the number of metabolically active cells [12]. Cell death has been measured from the LDH activity, and the number of metabolically active cells has been measured from the proliferation results observed in the WST-1 assay.…”

Section: Surfacementioning

confidence: 99%

“…To the best of our knowledge, there has only been one study recently published by our group on the electrochemical reduction of graphene oxide on CoCr-based alloys [11]. As was reported in [12], the electrochemical deposition of reduced graphene oxide (ErGO) on a biomedical grade CoCr produced a considerable increase in the macrophages' biocompatibility.…”

Section: Introductionmentioning

confidence: 97%

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Corrosion Behaviour and J774A.1 Macrophage Response to Hyaluronic Acid Functionalization of Electrochemically Reduced Graphene Oxide on Biomedical Grade CoCr

Chico

Pérez-Maceda

José

et al. 2021

Metals

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Improvements in the lubrication of metal–metal joint prostheses are of great clinical interest in order to minimize the particles released during wear–corrosion processes. In this work, electrochemically reduced graphene oxide (ErGO) on CoCr was functionalized with hyaluronic acid (ErGOHA). Functionalization was carried out by soaking for 24 h in phosphate buffer saline (PBS) solution containing 3 g/L hyaluronic acid (HA). The corrosion performance of CoCrErGO and CoCrErGOHA surfaces was studied by electrochemical impedance spectroscopy (EIS) for 7 days in PBS. Biocompatibility and cytotoxicity were studied in mouse macrophages J774A.1 cell line by the measurement of mitochondrial activity (WST-1 assay) and plasma membrane damage (LDH assay). The inflammatory response was examined through TNF-α and IL-10 cytokines in macrophages culture supernatants, used as indicators of pro-inflammatory and anti-inflammatory responses, respectively. EIS diagrams of CoCrErGOHA revealed two time constants: the first one, attributed to the hydration and diffusion processes of the HA layer adsorbed on ErGO, and the second one, the corrosion resistance of ErGOHA/CoCr interface. Macrophage assays showed better behavior on CoCrErGOHA than CoCr and CoCrErGO surfaces based on their biocompatible, cytotoxic, and inflammatory responses. Comparative analysis of IL-10 showed that functionalization with HA induces higher values of anti-inflammatory cytokine, suggesting an improvement in inflammatory behavior.

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Section: Resultsmentioning

confidence: 99%

Section: Measurement Of Lactate Dehydrogenase Activitymentioning

confidence: 99%

Section: Surfacementioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Corrosion Behaviour and J774A.1 Macrophage Response to Hyaluronic Acid Functionalization of Electrochemically Reduced Graphene Oxide on Biomedical Grade CoCr

Chico

Pérez-Maceda

José

et al. 2021

Metals

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“…In recent years, GO-based composite materials have been introduced in tissue engineering. GO-based composite materials have relatively low toxicity and high osteoinductive efficiency that facilitates rapid cell growth with loading application in bone tissue engineering [19,20]. Different composite materials have been introduced to regenerate and repair defective bone in tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Development of Arabinoxylan-Reinforced Apple Pectin/Graphene Oxide/Nano-Hydroxyapatite Based Nanocomposite Scaffolds with Controlled Release of Drug for Bone Tissue Engineering: In-Vitro Evaluation of Biocompatibility and Cytotoxicity against MC3T3-E1

et al. 2020

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Fabrication of reinforced scaffolds to repair and regenerate defected bone is still a major challenge. Bone tissue engineering is an advanced medical strategy to restore or regenerate damaged bone. The excellent biocompatibility and osteogenesis behavior of porous scaffolds play a critical role in bone regeneration. In current studies, we synthesized polymeric nanocomposite material through free-radical polymerization to fabricate porous nanocomposite scaffolds by freeze drying. Functional group, surface morphology, porosity, pore size, and mechanical strength were examined through Fourier Transform Infrared Spectroscopy (FTIR), Single-Electron Microscopy (SEM), Brunauer-Emmet-Teller (BET), and Universal Testing Machine (UTM), respectively. These nanocomposites exhibit enhanced compressive strength (from 4.1 to 16.90 MPa), Young’s modulus (from 13.27 to 29.65 MPa) with well appropriate porosity and pore size (from 63.72 ± 1.9 to 45.75 ± 6.7 µm), and a foam-like morphology. The increasing amount of graphene oxide (GO) regulates the porosity and mechanical behavior of the nanocomposite scaffolds. The loading and sustained release of silver-sulfadiazine was observed to be 90.6% after 260 min. The in-vitro analysis was performed using mouse pre-osteoblast (MC3T3-E1) cell lines. The developed nanocomposite scaffolds exhibited excellent biocompatibility. Based on the results, we propose these novel nanocomposites can serve as potential future biomaterials to repair defected bone with the load-bearing application, and in bone tissue engineering.

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Macrophage proteomic analysis of covalent immobilization of hyaluronic acid and graphene oxide on CoCr alloy in a tribocorrosive environment

Sánchez‐López,

Chico,

García‐Alonso

et al. 2024

J Biomedical Materials Res

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In this work, a sequential covalent immobilization of graphene oxide (GO) and hyaluronic acid (HA) is performed to obtain a biocompatible wear‐resistant nanocoating on the surface of the biomedical grade cobalt‐chrome (CoCr) alloy. Nanocoated CoCr surfaces were characterized by Raman spectroscopy and electrochemical impedance spectroscopy (EIS) in 3 g/L HA electrolyte. Tribocorrosion tests of the nanocoated CoCr surfaces were carried out in a pin on flat tribometer. The biological response of covalently HA/GO biofunctionalized CoCr surfaces with and without wear‐corrosion processes was studied through the analysis of the proteome of macrophages. Raman spectra revealed characteristic bands of GO and HA on the functionalized CoCr surfaces. The electrochemical response by EIS showed a stable and protective behavior over 23 days in the simulated biological environment. HA/GO covalently immobilized on CoCr alloy is able to protect the surface and reduce the wear volume released under tribocorrosion tests. Unsupervised classification analysis of the macrophage proteome via hierarchical clustering and principal component analysis (PCA) revealed that the covalent functionalization on CoCr enhances the macrophage biocompatibility in vitro. On the other hand, disruption of the HA/GO nanocoating by tribocorrosion processes induced a macrophage proteome which was differently clustered and was distantly located in the PCA space. In addition, tribocorrosion induced an increase in the percentage of upregulated and downregulated proteins in the macrophage proteome, revealing that disruption of the covalent nanocoating impacts the macrophage proteome. Although macrophage inflammation induced by tribocorrosion of HA/GO/CoCr surfaces is observed, it is ameliorated by the covalently grafting of HA, which provides immunomodulation by eliciting downregulations in characteristic pro‐inflammatory signaling involved in inflammation and aseptic loosening of CoCr joint arthroplasties. Covalent HA/GO nanocoating on CoCr provides potential applications for in vivo joint prostheses led a reduced metal‐induced inflammation and degradation by wear‐corrosion.

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Electrochemically reduced graphene oxide on CoCr biomedical alloy: Characterization, macrophage biocompatibility and hemocompatibility in rats with graphene and graphene oxide

Cited by 28 publications

References 29 publications

Corrosion Behaviour and J774A.1 Macrophage Response to Hyaluronic Acid Functionalization of Electrochemically Reduced Graphene Oxide on Biomedical Grade CoCr

Corrosion Behaviour and J774A.1 Macrophage Response to Hyaluronic Acid Functionalization of Electrochemically Reduced Graphene Oxide on Biomedical Grade CoCr

Development of Arabinoxylan-Reinforced Apple Pectin/Graphene Oxide/Nano-Hydroxyapatite Based Nanocomposite Scaffolds with Controlled Release of Drug for Bone Tissue Engineering: In-Vitro Evaluation of Biocompatibility and Cytotoxicity against MC3T3-E1

Macrophage proteomic analysis of covalent immobilization of hyaluronic acid and graphene oxide on CoCr alloy in a tribocorrosive environment

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