Electrochemically Driven Photosynthetic Electron Transport in Cyanobacteria Lacking Photosystem II

Lewis, Christine M.; Flory, Justin; Moore, Thomas A.; Moore, Ana L.; Rittmann, Bruce E.; Vermaas, Wim F. J.; Torres, César I.; Fromme, Petra

doi:10.1021/jacs.1c09291

Cited by 23 publications

(20 citation statements)

References 59 publications

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“…Rather than using electron mediators to enhance the EET, it is also possible to artificially internalize electrons to the photosynthetic pathway. This was successfully done by application of a negative potential bias of −0.249 V (vs. SHE) that can reduce exogenous duroquinone (DQ) into duroquinol (DQH 2 ) that can enter the cells and donate electrons to the plastoquinone pool in the photosynthetic pathway ( Lewis et al, 2022 ). Although the addition of exogenous electron mediators enhances the photocurrent, it may also increase the cost of the BEPC operation.…”

Section: Application Of Exogenous Electron Mediators Enhances the Pho...mentioning

confidence: 99%

“…While non-biological systems can tolerate very harsh conditions such as high temperature, organic solvent solutions, extreme pH, and high light intensities, biological-based electrochemical cells are limited by the environmental and physiological tolerance of the organisms (Zhang et al, 2018;Lewis et al, 2022). A very important technical factor that can enhance the electrical current production is the ionic strength of the electrolyte solution.…”

Section: Introductionmentioning

confidence: 99%

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Harnessing photosynthesis to produce electricity using cyanobacteria, green algae, seaweeds and plants

2022

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The conversion of solar energy into electrical current by photosynthetic organisms has the potential to produce clean energy. Life on earth depends on photosynthesis, the major mechanism for biological conversion of light energy into chemical energy. Indeed, billions of years of evolution and adaptation to extreme environmental habitats have resulted in highly efficient light-harvesting and photochemical systems in the photosynthetic organisms that can be found in almost every ecological habitat of our world. In harnessing photosynthesis to produce green energy, the native photosynthetic system is interfaced with electrodes and electron mediators to yield bio-photoelectrochemical cells (BPECs) that transform light energy into electrical power. BPECs utilizing plants, seaweeds, unicellular photosynthetic microorganisms, thylakoid membranes or purified complexes, have been studied in attempts to construct efficient and non-polluting BPECs to produce electricity or hydrogen for use as green energy. The high efficiency of photosynthetic light-harvesting and energy production in the mostly unpolluting processes that make use of water and CO2 and produce oxygen beckons us to develop this approach. On the other hand, the need to use physiological conditions, the sensitivity to photoinhibition as well as other abiotic stresses, and the requirement to extract electrons from the system are challenging. In this review, we describe the principles and methods of the different kinds of BPECs that use natural photosynthesis, with an emphasis on BPECs containing living oxygenic photosynthetic organisms. We start with a brief summary of BPECs that use purified photosynthetic complexes. This strategy has produced high-efficiency BPECs. However, the lifetimes of operation of these BPECs are limited, and the preparation is laborious and expensive. We then describe the use of thylakoid membranes in BPECs which requires less effort and usually produces high currents but still suffers from the lack of ability to self-repair damage caused by photoinhibition. This obstacle of the utilization of photosynthetic systems can be significantly reduced by using intact living organisms in the BPEC. We thus describe here progress in developing BPECs that make use of cyanobacteria, green algae, seaweeds and higher plants. Finally, we discuss the future challenges of producing high and longtime operating BPECs for practical use.

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Section: Application Of Exogenous Electron Mediators Enhances the Pho...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Harnessing photosynthesis to produce electricity using cyanobacteria, green algae, seaweeds and plants

2022

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“…Photoexcitation promotes P680 into excited state P680*, which is −0.66 V vs SHE. Spontaneous (downhill) electron transfer could then give photogenerated electrons to plastoquinone (PQ) (0.12 V)-Cyt bf (0.3 V) electron mediators, − and the electrons eventually transfer to P700 (0.41 V).…”

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confidence: 99%

Ultrafast Electron Transfer in Au–Cyanobacteria Hybrid for Solar to Chemical Production

Cui

et al. 2022

ACS Energy Lett.

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The rise of inorganic−biohybrid organisms for solarto-chemical production has spurred mechanistic investigations into the dynamics of the biotic−abiotic interface to drive the development of next-generation hybrid systems. The model system, cyanobacteria−gold nanoparticle hybrids, combines a light harvester with a photosynthetic bacterium to drive the reduction of CO 2 to glycerol with improved efficiency and increased glycerol production by 14.6%, in comparison to cyanobacteria only. In this work, we report insights into this unique photochemical behavior and propose a charge-transfer pathway from Au nanoparticle to cyanobacteria. Transient absorption (TA) spectroscopy revealed that photoexcited electron transfer rates are on the order of a few ps to the potential electron acceptor in photosystem II. This work represents a promising platform to utilize a conventional spectroscopic methodology to extract insights from more complex biotic−abiotic hybrid systems.

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“…In purple bacteria, intracellular nitrogenase catalyses nitrogen fixation and concurrently releases hydrogen (H2), a process that consumes electrons delivered by ferredoxin (Fd), the electron carrier that shuttles electrons from the photosynthetic electron transport chain to various downstream enzymes (e.g., nitrogenase and NADP reductase). 26 Since the catalytic activities of nitrogenase behaves differently under N2 and Ar atmosphere 27 , the light-driven H2 evolution for both the native cells and the biohybrid system was evaluated under N2 and Ar, respectively. The biohybrid system generated more H2 than the native cells under identical culture conditions for both N2 and Ar atmosphere (Fig.…”

Section: Metabolic Profiles Of R Rubrum-cds Biohybrid Systemmentioning

confidence: 99%

Semiconductor augumented valuable chemical photosynthesis fromRhodospirillum rubrumand mechanism study

Wang

Shu-lan

Liang

et al. 2023

Preprint

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Photosynthetic biohybrid systems based on purple bacteria and semiconducting nanomaterials are promising platforms for sustainable solar-powered chemical production. However, these types of biohybrid systems have not been fully developed to date, and their energy utilization and electron transfer mechanisms are not well understood. Herein, a Rhodospirillum rubrum-CdS biohybrid system was successfully constructed. The photosynthetic activity and photoelectrochemical properties of biohybrid system were analyzed. Chromatographic and spectroscopic studies confirmed the metabolic activities of R. rubrum cells were effectively augmented by surface-deposited CdS nanoparticles and validated with increased H2 evolution, polyhydroxybutyric acid (PHB) production, and solid biomass accumulation. Energy consumption and metabolic profiles of R. rubrum-CdS biohybrid system exhibited a growth phase-dependent behaviour. Photoelectrochemical study confirmed that light-excited electrons from CdS enhanced photosynthetic electron flow of R. rubrum cells. Monochromatic light modulated photoexcitation of biohybrid system was utilized to explore interfacial electron transfer between CdS and R. rubrum cells, and the results showed that CdS enhanced the utilization of blue light by R. rubrum cells. This work investigated the feasibility and prospect of utilizing R. rubrum in semi-artificial photosynthesis of valuable products, and offered insights into the energy utilization and the electron transfer mechanism between nanomaterials and purple bacteria.

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Electrochemically Driven Photosynthetic Electron Transport in Cyanobacteria Lacking Photosystem II

Cited by 23 publications

References 59 publications

Harnessing photosynthesis to produce electricity using cyanobacteria, green algae, seaweeds and plants

Harnessing photosynthesis to produce electricity using cyanobacteria, green algae, seaweeds and plants

Ultrafast Electron Transfer in Au–Cyanobacteria Hybrid for Solar to Chemical Production

Semiconductor augumented valuable chemical photosynthesis fromRhodospirillum rubrumand mechanism study

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