Electrochemically driven optical and SERS immunosensor for the detection of a therapeutic cardiac drug

Chaudhry, Madeeha; Lim, Dong‐Kwon; Kang, Jeon Woong; Yaqoob, Zahid; So, Peter T. C.; Bhopal, Muhammad Fahad; Wang, Minqiang; Qamar, Raheel; Bhatti, Arshad Saleem

doi:10.1039/d1ra07680a

Cited by 7 publications

(2 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fluorescence signal was proportional to the logarithmic concentration of digoxin in the range of 50 pM to 1.56 nM (y = 8622.6–12364lgx, R 2 =0.9867). The detection limit of digoxin was 25 pM, which is much lower than the permissible therapeutic concentration of digoxin in human plasma (1.28–2.56 nM) and better than some of the reported immunoassays. ,− The assay was selective for digoxin, and the simultaneous presence of other small molecules, including l -histidine, l -arginine, l -tyrosine, l -asparagine, and l -phenylalanine, did not interfere with the digoxin detection (Figure S9). The assay allowed the detection of digoxin in 20-fold diluted serum samples and 10-fold diluted urine samples.…”

Section: Resultsmentioning

confidence: 84%

CRISPR/Cas12a-Powered Competitive Immunosorbent Assay for Small Molecules

Zhu,

Zhao

2023

Anal. Chem.

View full text Add to dashboard Cite

CRISPR/Cas systems are powerful tools for sensitive nucleic acid molecular diagnosis due to their specific nucleic acid recognition and high transcleavage activity and have also allowed for quantification of non-nucleic acid targets, relying on a strategy to convert the target detection to analysis of nucleic acids. Here, we describe a CRISPR/Cas12a-powered immunosorbent assay for sensitive smallmolecule detection by using the antibody coated on the microplate to recognize the target and the small molecule-labeled active DNA (acDNA) to trigger the activity of CRISPR/Cas12a. In the absence of small-molecule targets, acDNA probes are captured by the antibody on the microplate and then activate Cas12a in catalytic trans-cleavage of fluorescent DNA reporters, generating strong fluorescence. The presence of smallmolecule targets displaces the acDNA probes from the antibody, causing a decrease of acDNA probes on the microplate and reduction of activated Cas12a, so the fluorescence signal decreases, and small molecules can be detected by monitoring the fluorescence change. After systematically optimizing experimental conditions (e.g., Cas12a reaction), the proposed method achieved the detection of three model small molecules, biotin, digoxin, and folic acid, with low detection limits, and a flexible detection concentration range was obtained by simply changing the amount of acDNA probes and immobilized antibodies. The assay showed high selectivity and good applicability in complex media. The integration of the CRISPR/Cas12a system improves the analytical performance of immunoassay, broadening and facilitating its applications in rapid, simple, and sensitive small molecule analysis.

show abstract

Section: Resultsmentioning

confidence: 84%

CRISPR/Cas12a-Powered Competitive Immunosorbent Assay for Small Molecules

Zhu,

Zhao

2023

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…Large-scale fabrication of an ordered array of plasmonic nanoparticles is of profound interest in multiple areas of research including nanophotonics and biomedical sciences. − Such arrays utilize the unique optical and electronic properties of metallic nanostructures to confine light to subwavelength volumes, particularly in narrow gaps between metal nanoparticles, and as a result, uniformly distributed high-intensity electromagnetic field regions called “plasmonic hotspots” are generated. ,, Such uniform hotspots are vital for surface-enhanced Raman scattering (SERS), which is one of the most promising analytical techniques that combine high molecular specificity with detection sensitivity down to the single-molecule level. , SERS has shown promise for a myriad of applications ranging from biomedical diagnosis and imaging to environmental monitoring, food safety, and forensic analysis. − However, its adoption for practical applications remains largely elusive, primarily due to the limited uniformity and controllability of hotspots in available SERS substrates. Indeed, the intractability of controlling enhancement has led to theorization of a “SERS-uncertainty principle” .…”

Section: Introductionmentioning

confidence: 99%

Self-Assembled Bimetallic Au–Ag Nanorod Vertical Array for Single-Molecule Plasmonic Sensing

Tanwar,

Anantha,

et al. 2024

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

Ordered plasmonic nanoparticle arrays are highly desirable for optical sensing as they provide uniformly distributed plasmonic hotspots due to their periodic order and near-field coupling. Anisotropic-shaped bimetallic nanoparticles are of particular interest, as their hybridized plasmonic modes enable precise tuning of plasmonic resonance and optical responses. However, the controlled assembly of large-scale arrays of bimetallic nanoparticles with uniformly distributed hotspots remains a challenge. In this study, we present a highly robust and reproducible method for creating large-area vertically aligned arrays of bimetallic Au−Ag nanorods by epitaxially growing Ag over preassembled Au nanorods. Structural characterization using electron microscopy and X-ray photoelectron spectroscopy confirms the formation of a uniform thin layer of Ag, creating a bimetallic Au−Ag nanorod array. We also demonstrate the efficacy of the designed nanoarrays for surface-enhanced Raman scattering (SERS) spectroscopy. Our experimental and computational studies show considerably enhanced optical responses of bimetallic Au−Ag nanorods compared to their monometallic counterparts. The scalability, cost-effectiveness, and reproducibility of this method make it a versatile platform for creating various structures by varying guest nanoparticles in suspensions with broad applications in biomedical research, food safety surveillance, and environmental monitoring.

show abstract

Simple synthesis of MOF-derived Zn, Co electrocatalyst for sensitive detection of digoxin in urine sample

Manjula

Pulikkutty

Chen

2023

Colloids and Surfaces A: Physicochemical and Engineering Aspect

View full text Add to dashboard Cite

Electrochemically driven optical and SERS immunosensor for the detection of a therapeutic cardiac drug

Abstract: Cardiovascular diseases pose a serious health risk and have a high mortality rate of 31% worldwide.

Cited by 7 publications

References 51 publications

CRISPR/Cas12a-Powered Competitive Immunosorbent Assay for Small Molecules

CRISPR/Cas12a-Powered Competitive Immunosorbent Assay for Small Molecules

Self-Assembled Bimetallic Au–Ag Nanorod Vertical Array for Single-Molecule Plasmonic Sensing

Simple synthesis of MOF-derived Zn, Co electrocatalyst for sensitive detection of digoxin in urine sample

Contact Info

Product

Resources

About