Electrochemical/visual dual-readout aptasensor for Ochratoxin A detection integrated into a miniaturized paper-based analytical device

Zhang, Xiaobo; Zhi, Hui; Zhu, Mingzhen; Wang, Fengya; Meng, Hu; Feng, Liang

doi:10.1016/j.bios.2021.113146

Cited by 73 publications

(24 citation statements)

References 50 publications

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“…EPads are always sensitive and have quick response, which have been a main support at the POCT. Recent examples of applications are reviewed here to demonstrate the potential of ePads in environmental monitoring [ 49 ] and biomedical analysis [ 52 – 78 ] as well as food safety control [ 79 – 81 ] field.…”

Section: Electrochemical Detection Methodsmentioning

confidence: 99%

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Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Hou

Guo

et al. 2022

J. Anal. Test.

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Point-of-care testing (POCT), as a portable and user-friendly technology, can obtain accurate test results immediately at the sampling point. Nowadays, microfluidic paper-based analysis devices (μPads) have attracted the eye of the public and accelerated the development of POCT. A variety of detection methods are combined with μPads to realize precise, rapid and sensitive POCT. This article mainly introduced the development of electrochemistry and optical detection methods on μPads for POCT and their applications on disease analysis, environmental monitoring and food control in the past 5 years. Finally, the challenges and future development prospects of μPads for POCT were discussed.

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Section: Electrochemical Detection Methodsmentioning

confidence: 99%

“…In Zhang’s work [ 79 ], Ochratoxin A (OTA) was used as the model for an ePad immunoassay. Functionalized MoS 2 -Au@Pt (Ch-MoS 2 -Au@Pt) was produced to immobilize label aptamer (apta2) for signal amplification.…”

Section: Electrochemical Detection Methodsmentioning

confidence: 99%

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Hou

Guo

et al. 2022

J. Anal. Test.

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“…In addition to the above detection methods, cyclic voltammetry (CV) and alternating current voltammetry (ACV) were also been used in measuring the electrochemical signal. Feng et al developed a biosensor based on the aptamer biorecognition for OTA detection, using CV detection method [69]. The capture aptamer was anchored on the Au NPs supported MnO 2 nanoflower by AuÀ S bond.…”

Section: Impedance Biosensors For Mycotoxinsmentioning

confidence: 99%

Mycotoxins Detection Based on Electrochemical Approaches

et al. 2021

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Electrochemical biosensors have attracted much attention in mycotoxin bioanalysis. In this review, three electrochemical biosensor technologies for mycotoxins were reviewed, including general electrochemistry, photoelectrochemistry, and electrochemiluminescence. Based on the classification of multiple electrochemical detection methods, the design schemes, recognition mechanism and probe materials were described in detail. Moreover, the characteristics and limitations of these electrochemical biosensors were summarized. The challenges and future trends of electrochemical biosensor development in mycotoxin bioanalysis were also briefly discussed in the end. This review is expected to provide some inspirations for point-of-care testing in electrochemical sensors for mycotoxins and further electrochemical analysis application.

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“…Among the most relevant points in the use of this material, there are advantages such as biocompatibility and biodegradability, low cost, and ease of production [12]. These aspects have led to a growing interest in the development of paper-based analytical devices (PADs), such as smart labels [13], gas sensors [14,15], and sensors combining electrochemical and visual readouts [16]. PADs have successfully found application in diagnostics [4], environmental monitoring [17], and food control [18].…”

Section: Introductionmentioning

confidence: 99%

Paper-based aptamer-antibody biosensor for gluten detection in a deep eutectic solvent (DES)

et al. 2021

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Paper has been widely employed as cheap material for the development of a great number of sensors such as pregnancy tests, strips to measure blood sugar, and COVID-19 rapid tests. The need for new low-cost analytical devices is growing, and consequently the use of these platforms will be extended to different assays, both for the final consumer and within laboratories. This work describes a paper-based electrochemical sensing platform that uses a paper disc conveniently modified with recognition molecules and a screen-printed carbon electrode (SPCE) to achieve the detection of gluten in a deep eutectic solvent (DES). This is the first method coupling a paper biosensor based on aptamers and antibodies with the DES ethaline. Ethaline proved to be an excellent extraction medium allowing the determination of very low gluten concentrations. The biosensor is appropriate for the determination of gluten with a limit of detection (LOD) of 0.2 mg L−1 of sample; it can detect gluten extracted in DES with a dynamic range between 0.2 and 20 mg L−1 and an intra-assay coefficient of 10.69%. This approach can be of great interest for highly gluten-sensitive people, who suffer from ingestion of gluten quantities well below the legal limit, which is 20 parts per million in foods labeled gluten-free and for which highly sensitive devices are essential. Graphical abstract

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Electrochemical/visual dual-readout aptasensor for Ochratoxin A detection integrated into a miniaturized paper-based analytical device

Cited by 73 publications

References 50 publications

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Mycotoxins Detection Based on Electrochemical Approaches

Paper-based aptamer-antibody biosensor for gluten detection in a deep eutectic solvent (DES)

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