Electrochemical-surface enhanced Raman spectroscopy (E-SERS) of uric acid: a potential rapid diagnostic method for early preeclampsia detection

Goodall, Barbara L.; Robinson, Ashley M.; Brosseau, Christa L.

doi:10.1039/c2cp42596c

Cited by 68 publications

(53 citation statements)

References 52 publications

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“…This has been observed in this work (Fig. ) and in some previous studies . This makes traces of uric acid in biological fluids difficult to identify by means of SERS spectroscopy.…”

Section: Resultssupporting

confidence: 74%

“…The most significant spectral changes can be explained by tautomerization/deprotonation of uric acid molecules in aqueous solutions and while interacting with the silver surface . In addition, SERS enhancement is subject to the surface selection rules and is different for each vibrational mode .…”

Section: Resultsmentioning

confidence: 99%

“…Electrochemical SERS (e‐SERS) was proposed for detection of uric acid in aqueous solutions as well as in urine stimulant . The method was capable of detecting uric acid concentrations down to 0.1 mM, and little interference from other components was observed in the urine stimulant.…”

Section: Introductionmentioning

confidence: 99%

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Uric acid detection by means of SERS spectroscopy on dried Ag colloidal drops

Pučetaitė

Velička

Pilipavičius

et al. 2016

J. Raman Spectrosc.

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Uric acid concentration in human bodily fluids is an important marker for disorders such as gout, pre‐eclamsia or cardiovascular disease. However, currently used methods for its detection either lack sensitivity or require sophisticated, bulky and expensive equipment. In this work, we show that by using surface‐enhanced Raman scattering spectroscopy (SERS) on dried Ag colloidal drops and with 1064 nm excitation, concentrations of uric acid in aqueous solutions down to 10−6 M can be detected. Such sensitivity is sufficient for medical applications as concentration of uric acid in various bodily fluids are in the range of 10−3–10−4 M. Drying of the colloidal drops is known to result in the formation of ‘coffee‐ring’ structures that allow obtaining high enhancements but poor reproducibility. Here, the formation of the structures was avoided by choosing aluminum oxide as a base substrate and by controlling environment conditions. Despite the fact that variations of signal enhancement from sample to sample prevent quantitative analysis from being performed, the results of this work imply that strict control of sample preparation conditions could lead to obtaining reproducible SERS enhancements. Results of density functional theory calculations of uric acid tautomer – five‐atom silver cluster complexes performed for the first time show that the differences between Raman and SERS spectra of uric acid can be mainly explained by tautomerization of the molecule and its bonding to the silver surface. Assignment of spectral bands is important for correct SERS signal interpretation and detection of uric acid in biological fluids in the future studies. Copyright © 2016 John Wiley & Sons, Ltd.

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“…This has been observed in this work (Fig. ) and in some previous studies . This makes traces of uric acid in biological fluids difficult to identify by means of SERS spectroscopy.…”

Section: Resultssupporting

confidence: 74%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Uric acid detection by means of SERS spectroscopy on dried Ag colloidal drops

Pučetaitė

Velička

Pilipavičius

et al. 2016

J. Raman Spectrosc.

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“…4). It has been reported in the literature that, when in contact with the metal surface, the uric acid molecules undergoes deprotonation and forms dianionic species [31] which also explains the alterations in the spectra. Cystine and urea samples produced SERS spectra enhanced up to 10 2 orders of magnitude.…”

Section: Resultsmentioning

confidence: 98%

Application of SERS spectroscopy for detection of trace components in urinary deposits

Pučetaitė

Velička

Tamošaitytė

et al. 2014

Plasmonics in Biology and Medicine XI

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Surface-enhanced Raman scattering (SERS) spectroscopy can be a useful tool in regard to disease diagnosis and prevention. Advantage of SERS over conventional Raman spectroscopy is its significantly increased signal (up to factor of 10 6 -10 8 ) which allows detection of trace amounts of substances in the sample. So far, this technique is successfully used for analysis of food, pieces of art and various biochemical/biomedical samples. In this work, we survey the possibility of applying SERS spectroscopy for detection of trace components in urinary deposits. Early discovery together with the identification of the exact chemical composition of urinary sediments could be crucial for taking appropriate preventive measures that inhibit kidney stone formation or growth processes. In this initial study, SERS spectra (excitation wavelength -1064 nm) of main components of urinary deposits (calcium oxalate, uric acid, cystine, etc.) were recorded by using silver (Ag) colloid. Spectra of 10 -3 -10 -5 M solutions were obtained. While no/small Raman signal was detected without the Ag colloid, characteristic peaks of the substances could be clearly separated in the SERS spectra. This suggests that even small amounts of the components could be detected and taken into account while determining the type of kidney stone forming in the urinary system. We found for the first time that trace amounts of components constituting urinary deposits could be detected by SERS spectroscopy. In the future study, the analysis of centrifuged urine samples will be carried out.

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“…But there were interference peaks at 999 and 1030 cm -1 in the blank urine sample, which may be caused by the C-N stretching vibration of the urea 43 and the mixed vibration of uric acid. 44,45 Therefore, we combined the characteristic peaks of BH molecules at 826 cm -1 to analyze the detection limit of BH in urine instead of simply considering 0.01 μg/mL as the detection limit. When the concentration of the solution was low to 0.1 μg/mL, the characteristic peak signal at 826 cm -1 disappeared in the spectrum because of the competitive adsorption 46 of urine and BH molecules on silver nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

Detection and Quantification of Bucinnazine Hydrochloride Injection Based on SERS Technology

Zhang¹,

Han

Zhao

et al. 2018

ANAL. SCI.

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In this investigation, surface-enhanced Raman spectroscopy (SERS) technology was performed to detect bucinnazine hydrochloride (BH) injection in water and urine. The theoretical Raman spectrum of BH with characteristic peaks was calculated and identified by density functional theory (DFT). Employing an improved silver sol as a SERS active substrate, the SERS spectra of a BH solution with different concentrations were acquired with a 0.5 mol/L KI solution as an aggregation agent. It was determined that the limit of detection (LOD) was low, to 0.01 μg/mL. A good linear relationship of BH between the Raman intensity and the concentrations was obtained in water at a concentration range from 0.5 to 6 μg/mL (R = 0.9914), which laid a favorable foundation for quantitative analysis. In addition, the recovery rate of spiked samples from 95.13 to 120.54% were calculated. Finally, the detection of BH injection in artificial urine was completed and the detection limit could reach 0.5 μg/mL, which met the requirements of a rapid on-site detection of drugs in urine. As a result, it indicates that the inspection of BH by the SERS method is with simplicity and high sensitivity, having a great potential for real-time detection.

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Electrochemical-surface enhanced Raman spectroscopy (E-SERS) of uric acid: a potential rapid diagnostic method for early preeclampsia detection

Cited by 68 publications

References 52 publications

Uric acid detection by means of SERS spectroscopy on dried Ag colloidal drops

Uric acid detection by means of SERS spectroscopy on dried Ag colloidal drops

Application of SERS spectroscopy for detection of trace components in urinary deposits

Detection and Quantification of Bucinnazine Hydrochloride Injection Based on SERS Technology

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