Electrochemical study of the effect of nano-zinc oxide on microperoxidase and its application to more sensitive hydrogen peroxide biosensor preparation

Zhu, Xiaoli; Ishida, Yasuhiro; Gan, Xin; Suzuki, I.; Li, Genxi

doi:10.1016/j.bios.2006.07.007

Cited by 180 publications

(85 citation statements)

References 13 publications

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“…Wang et al 5 prepared ZnO nanocombs to immobilize glucose oxidase. Zhu et al 6 used ZnO nanoparticles to investigate electrochemistry of microperoxidase. Yan et al 7 fabricated ZnO based acoustic resonator as mass sensor by multitarget magnetron sputtering under optimized deposition conditions.…”

Section: -3mentioning

confidence: 99%

Nanostructured zinc oxide platform for cholesterol sensor

Solanki¹,

Ansari²,

Malhotra³

2009

Applied Physics Letters

109

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Nanostructured zinc oxide ͑nano-ZnO͒ film has been fabricated onto indium tin oxide ͑ITO͒ containing preferred ͑002͒ plane and 10 nm crystallite size using sol-gel technique for immobilization of cholesterol oxidase ͑ChOx͒. Electrochemical response of ChOx/nano-ZnO/ITO bioelectrode determined as a function of cholesterol concentration using cyclic voltammetry technique reveals improved detection range ͑5-400 mg/dl͒, low detection limit ͑0.5 mg/dl͒, fast response time ͑10 s͒, sensitivity ͑0.059 A / mg dl −1 cm −2 ͒, and low value ͑0.98 mg/dl͒ of Michaelis-Menten constant ͑K m ͒. It is shown that nano-ZnO film provides better environment and enhanced electron transfer between ChOx and electrode. © 2009 American Institute of Physics. ͓DOI: 10.1063/1.3111429͔ Nanostructured metal oxide films such as zinc oxide ͑ZnO͒, cerium oxide, and titanium oxide have recently attracted much attention due to their interesting optical, electrical, and molecular properties for biosensor applications. [1][2][3] Among these, ZnO nanostructured film due to wide band gap ͑3.37 eV͒, large excitation binding energy ͑60 eV͒, high surface area, nontoxicity, good biocompatibility, chemical stability, and high electron communication feature is preferred for development of biosensors for clinical diagnostics. 2 The high isoelectric point ͑IEP͒ of ϳ9.5 for ZnO is advantageous for enhanced electrostatic interaction with enzymes having low IEP.Krishnamoorthy et al. 4 developed surface acoustic wave biosensor to investigate characteristics of cytokine immobilized ZnO and SiO 2 films grown onto ͑100͒ Si substrates. Wang et al. 5 prepared ZnO nanocombs to immobilize glucose oxidase. Zhu et al. 6 used ZnO nanoparticles to investigate electrochemistry of microperoxidase. Yan et al. 7 fabricated ZnO based acoustic resonator as mass sensor by multitarget magnetron sputtering under optimized deposition conditions. Wei et al. 8 utilized ZnO nanorods array for application to glucose biosensor. Meulenkamp et al. 9 prepared sol-gel based ZnO matrix for a mediator-free tyrosinase biosensor. The cholesterol biosensor based on rf sputtered nanoporous ZnO/Au film 2 shows high K m , poor detection limit and is expensive. ChOx/chitosan-ZnO bioelectrode shows K m as 0.223 mM, linearity up to 300 mg/dl and shelf life of 8 weeks. 10 The sol-gel derived nanostructured metal oxide films due to better thermal stability, low cost, biocompatibility, nontoxicity, tunable porosity, low temperature processing, etc. have attracted much interest for biosensing application. 11 We report results of studies relating to sol-gel derived nano-ZnO film deposited onto indium-tin-oxide ͑ITO͒ substrate for application to cholesterol sensor.Zinc acetate hydrate ͑1 g͒ is dissolved in 20 ml ethanol at 25°C. Then 5 ml ͑1M͒ solution of ammonium hydroxide is added drop wise to this solution with constant stirring for 1h at 25°C to maintain pH 10. A white precipitate of Zn͑OH͒ 2 obtained is washed several times with de-ionized water until neutral pH is reached. Subsequently, dilute HNO 3 ͑1...

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Section: -3mentioning

confidence: 99%

Nanostructured zinc oxide platform for cholesterol sensor

Solanki¹,

Ansari²,

Malhotra³

2009

Applied Physics Letters

109

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“…All of these properties make ZnO an important functional oxide. Due to its fast electron transfer capability, ZnO is a key material for fabrication of biomembranes, and enzymatic detective devices [18]. Nano-zinc oxide is a key element for maintenance of the structural and functional integrity of eukaryotic cells and tissues [19].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Nano-doped Zinc Oxide and its Application as Protective Oxidative Changes in the Retina of Diabetic Rats

El-Rahman¹,

Sm²,

Al-Ghannam³

2016

J Diabetes Metab

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Oxidative stress is a basic mechanism behind the development of diabetic retinopathy (DR). Therefore, the present study conducted to evaluate the protective effect of zinc oxide nanoparticles (ZnONPs) doped with fluorine (F:ZnO) and chlorine (Cl:ZnO), on retinal oxidative stress in type II diabetic rats. TEM showed that the F:ZnO and Cl:ZnO have average particle size 17.7 and 59.3 nm, respectively. The results indicated that serum glucose was increased significantly (p ≤ 0.05) and serum insulin was decreased significantly 3 days after treatment with STZ compared to other groups. STZ treatment depleted both retinal and liver TBA and retinal GST contents. While, simultaneous treatment rats with low and high dose of STZ + F:ZnO and STZ + Cl:ZnO were decreased serum glucose induced by STZ and reversed the deplete influence on GSH level of retinal and reduced the highs in TBA levels of livers and retinas in STZ -administrated rats. The results investigate the useful effects of high dose of F:ZnO and Cl:ZnO in protection diabetic rats against hyperglycemia and retina against oxidative stress.

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“…33,34 In addition, third-generation PEC enzyme biosensors based on direct electrochemistry have been shown to be capable of direct electron transfer between their active sites and electrodes. 33,35,36 A number of enzymes/redox proteins, such as horseradish peroxidase, 37 hemoglobin, 38 microperoxidase 39 and glucose oxidase, 40,41 have been integrated into enzyme-based, third-generation biosensors. Recent studies have revealed the possibilities of switching photocurrent (cathodic or anodic) based on the direct electrochemistry of cytochrome c (Cyt c) for sensitive biosignal monitoring.…”

Section: Introductionmentioning

confidence: 99%

Interfacial assembly of mesoporous nanopyramids as ultrasensitive cellular interfaces featuring efficient direct electrochemistry

et al. 2015

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A general method for assembling patterned interfaces of uniform, flexible mesoporous iron oxide nanopyramid islands (NPIs) is presented. The three-dimensional (3D) mesoporous iron oxide-NPI interfaces possess a unique mesostructure that features a large surface area (~158 m 2 g − 1 ), a large pore size (~18 nm) and excellent flexibility (can be folded 100 times). Furthermore, the 3D mesoporous Au-NPI interfaces allow efficient immobilization of cytochrome c (Cyt c; more than 165-fold increase) and a significant enhancement of localized surface plasmon resonance (~26-fold at 625 nm) compared with that of two-dimensional (2D) planar iron oxide films without nanopores. More importantly, the ultrasensitive integrated interfaces demonstrate over 1000-fold enhancement of the photocurrent variation on the 3D mesostructures based on the switchable direct electrochemistry of Cyt c. The strategy of interfacial assembly offers new possibilities for the chemical design of patterned mesoporous semiconductors with high flexibility and tailored photocatalytic characteristics. This investigation provides a novel paradigm for an unconventional 3D porous biointerface that can be used for sub-nanomolar level recognition of biomolecules (~0.2 nM for H 2 O 2 ) and suggests the new concept of large-surface-area 3D mesostructure-protein interfaces as a step toward using direct electrochemistry for biomedical applications.

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Electrochemical study of the effect of nano-zinc oxide on microperoxidase and its application to more sensitive hydrogen peroxide biosensor preparation

Cited by 180 publications

References 13 publications

Nanostructured zinc oxide platform for cholesterol sensor

Nanostructured zinc oxide platform for cholesterol sensor

Synthesis and Characterization of Nano-doped Zinc Oxide and its Application as Protective Oxidative Changes in the Retina of Diabetic Rats

Interfacial assembly of mesoporous nanopyramids as ultrasensitive cellular interfaces featuring efficient direct electrochemistry

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