Electrochemical study of d-glucose oxidase autoinactivation

Bourdillon, Christian; Thomas, Vaughan; Thomas, Daniel

doi:10.1016/0141-0229(82)90113-2

Cited by 63 publications

(34 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…26,27 Instrumentation and software Constant potential amperometry was performed in all experiments using a low-noise potentiostat (Biostat II, Electrochemical and Medical systems, Newbury, UK). Data acquisition was carried out with a Gateway GP6-350 computer, a Powerlab/400 interface system (ADInstruments Ltd, East Sussex, UK) and Chart for Windows (v4.0.1) software (ADInstruments Ltd).…”

Section: Nafion ® Application: Pre-cast Methodsmentioning

confidence: 99%

Microelectrochemical sensors for in vivo brain analysis: an investigation of procedures for modifying Pt electrodes using Nafion^®

Brown

Lowry

2003

Analyst

View full text Add to dashboard Cite

Various Nafion ® coating procedures were examined in order to design a simple and reproducible coating method to maximise permselective characteristics, and thus eliminate signals from electroactive interferents, in sensors designed for direct in vivo measurements in the brain. Interferents investigated included ascorbic acid (AA), the principal endogenous electroactive interferent present in the brain, and uric acid. Application of the Nafion ® (5% commercial solution) using a thermally annealing procedure involving 5 pre-coats, and 2 subsequent dip-bake layers resulted in elimination of interferent signals. It also produced complete blocking of the signal for the neurotransmitter dopamine. The optimum time and temperature for annealing was found to be 5 min at 210 °C. An examination of shelf life over two weeks indicated negligible AA interference over this period. Preliminary investigations with respect to the potential use of these Nafion ® -modified Pt electrodes in the design of implantable, first generation, peroxide detecting biosensors indicated that the modified electrode had no effect on O 2 permeability but did produce a significant decrease in H 2 O 2 sensitivity. While this may preclude their use in biosensor development they may be more suitable for detection of gaseous neurochemicals such as nitric oxide.

show abstract

Section: Nafion ® Application: Pre-cast Methodsmentioning

confidence: 99%

Microelectrochemical sensors for in vivo brain analysis: an investigation of procedures for modifying Pt electrodes using Nafion^®

Brown

Lowry

2003

Analyst

View full text Add to dashboard Cite

show abstract

“…A similar lack of O 2 interference has also been observed by other groups; Asai et al (1996) have used glutamate oxidase and a dialysis electrode incorporating a PPD coated Pt wire to monitor brain extracellular glutamate during acute ischemia and found that the sensor operates reliably despite the lack of O 2 around the dialysis electrode and Garguilo and Michael (1994) have found that the availability of O 2 in brain ECF is sufficient for from the reaction of O 2 , the natural cosubstrate for GOx, with the reduced form of the enzyme (GOx/ FADH 2 ) which is generated by reaction with glucose (see reactions 1 and 2 above). We have previously demonstrated in vitro that these biosensors respond to glucose in O 2 -depleted (N 2 -saturated) solutions with a sensitivity similar to that of air-saturated media (200 vM O 2 ) (Bourdillon et al, 1982;Zhang and Wilson, 1993), suggesting that in brain tissue where the range of O 2 tension is 5-50 vM (Feng et al, 1988;Zimmerman and Wightman, 1991), glucose monitoring should be free of O 2 interference (Lowry et al, 1994b).…”

Section: Effect Of Oxygen On Biosensor Response In 6i6omentioning

confidence: 98%

“…Post in vivo calibrations for AA and O 2 were carried out using the two techniques. Calibrations for dissolved O 2 were carried out in N 2 purged, air-saturated and O 2 -saturated solutions where the concentrations of solution O 2 were taken as 0, 200 (Bourdillon et al, 1982;Zhang and Wilson, 1993) and 1250 vM (Bourdillon et al, 1982), respectively. AA calibrations were carried out in the range 0-1 mM.…”

Section: Voltammetric Techniques In 6i6omentioning

confidence: 99%

An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats

Lowry

Miele

O’Neill

et al. 1998

Journal of Neuroscience Methods

100

View full text Add to dashboard Cite

Amperometric glucose biosensors based on the immobilization of glucose oxidase (GOx) on Pt electrodes with electropolymerized o-phenylenediamine (PPD) were implanted in the right striatum of freely-moving rats. Carbon paste electrodes for the simultaneous monitoring of ascorbic acid (AA) and/or tissue O 2 were implanted in the left striatum. A detailed in vivo characterization of the Pt/PPD/GOx signal was carried out using various pharmacological manipulations. Confirmation that the biosensor responded to changing glucose levels in brain extracellular fluid (ECF) was obtained by intraperitoneal (i.p.) injection of insulin that caused a decrease in the Pt/PPD/GOx current, and local administion of glucose (1 mM) via an adjacent microdialysis probe that resulted in an increase in the biosensor current. An insulin induced increase in tissue O 2 in the brain was also observed. Interference studies involved administering AA and subanaesthetic doses of ketamine i.p. Both resulted in increased extracellular AA levels with ketamine also causing an increase in O 2 . No significant change in the Pt/PPD/GOx current was observed in either case indicating that changes in O 2 and AA, the principal endogenous interferents, have minimal effect on the response of these first generation biosensors. Stability tests over a successive 5-day period revealed no significant change in sensitivity. These in vivo results suggest reliable glucose monitoring in brain ECF.

show abstract

“…For post in-vivo calibrations involving constant potential amperometry (CPA), where the detecting electrode is held at a constant potential sufficient to detect the oxidation or reduction of the target substance, the same three-electrode (sensor, reference and auxiliary) configuration of CFEs was maintained and the concentrations of solution O 2 taken as 0 μM (N 2 -saturated), 200 μM (air saturated (Bourdillon et al, 1982;Zhang and Wilson, 1993)) and 1260 μM (O 2 -saturated (Bourdillon et al, 1982)), respectively.…”

Section: Electrochemical Experiments In Vitromentioning

confidence: 99%

Real-time electrochemical monitoring of brain tissue oxygen: A surrogate for functional magnetic resonance imaging in rodents

et al. 2010

View full text Add to dashboard Cite

Long-term in-vivo electrochemistry (LIVE) enables real-time monitoring and measurement of brain metabolites. In this study we have simultaneously obtained blood oxygenation level dependent (BOLD) fMRI and amperometric tissue O 2 data from rat cerebral cortex, during both increases and decreases in inspired O 2 content. BOLD and tissue O 2 measurements demonstrated close correlation (r = 0.7898) during complete (0%) O 2 removal, with marked negative responses occurring ca. 30 s after the onset of O 2 removal. Conversely, when the inspired O 2 was increased (50, 70 and 100% O 2 for 1 min) similar positive rapid changes (ca. 15 s) in both the BOLD and tissue O 2 signals were observed. These findings demonstrate, for the first time, the practical feasibility of obtaining real-time metabolite information during fMRI acquisition, and that tissue O 2 concentration monitored using an O 2 sensor can serve as an index of changes in the magnitude of the BOLD response. As LIVE O 2 sensors can be used in awake animals performing specific behavioural tasks the technique provides a viable animal surrogate of human fMRI experimentation.

show abstract

Electrochemical study of d-glucose oxidase autoinactivation

Cited by 63 publications

References 22 publications

Microelectrochemical sensors for in vivo brain analysis: an investigation of procedures for modifying Pt electrodes using Nafion^®

Microelectrochemical sensors for in vivo brain analysis: an investigation of procedures for modifying Pt electrodes using Nafion^®

An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats

Real-time electrochemical monitoring of brain tissue oxygen: A surrogate for functional magnetic resonance imaging in rodents

Contact Info

Product

Resources

About

Electrochemical study of d-glucose oxidase autoinactivation

Cited by 63 publications

References 22 publications

Microelectrochemical sensors for in vivo brain analysis: an investigation of procedures for modifying Pt electrodes using Nafion®

Microelectrochemical sensors for in vivo brain analysis: an investigation of procedures for modifying Pt electrodes using Nafion®

An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats

Real-time electrochemical monitoring of brain tissue oxygen: A surrogate for functional magnetic resonance imaging in rodents

Contact Info

Product

Resources

About

Microelectrochemical sensors for in vivo brain analysis: an investigation of procedures for modifying Pt electrodes using Nafion^®

Microelectrochemical sensors for in vivo brain analysis: an investigation of procedures for modifying Pt electrodes using Nafion^®