Electrochemical Studies on Nitrite Reductase toward a Biosensor

Investigation of the electrochemical behavior of metallo-tetraazaporphyrin modified silver and pyrolytic graphite electrodes in aqueous nitrite solution

“…It is achieved with the help of nitrite reductase enzymes, which contain complex proteins having an Fe-S unit and an iron-isobactereochlorin [7][8][9][10]. The process in-0022-0728/$ -see front matter Ó 2004 Elsevier B.V. All rights reserved.…”

Protein Film Voltammetry Reveals Distinctive Fingerprints of Nitrite and Hydroxylamine Reduction by a Cytochrome c Nitrite Reductase

“…The differences between the absolute responses recorded in each substrate will reflect differences in the rate constants and reduction potentials specific to the catalytic cycle describing reduction of each substrate. Interestingly, our inspection, of the solution voltammetry of D. desulfuricans cytochrome c nitrite reductase under conditions supporting a maximum nitrite reduction rate and measured with a view to development of a nitrite biosensor, shows a catalytic waveform of similar shape to that observed during PFV of the E. coli enzyme (19,21). This suggests that the description of enzyme-limited substrate reduction uncovered by PFV will be common to the cytochrome c nitrite reductases, in agreement with the conservation of active site structure and heme packing motifs recently revealed by structural studies of this important family of enzymes.…”

A novel nitrite biosensor based on conductometric electrode modified with cytochrome c nitrite reductase composite membrane

“…ccNiR exhibits several advantages as the biorecognition element of a nitrite biosensor: well-defined chemical reaction, high-specific activity, good stability and large yield . The first example of a nitrite biosensor based on ccNiR was reported by Scharf et al (1995). Strehlitz et al (1996) reported a list of possible artificial redox mediators that shuttle electrons from ccNiR to the electrode, thus allowing the development of mediated biosensors.…”

“…One of the most popular configurations of biosensors consists of enzyme immobilized within a polymer on an electrode. NiRs are natural candidates to build specific nitrite biosensors (Scharf et al, 1995;Wu et al, 1997). NiRs may be classified by their reaction products (NO or NH 4 + ) or their prosthetic groups (siroheme, heme c, heme cdl, copper, etc.…”