Electrochemical Studies of Morpholino-DNA Surface Hybridization

O'Connor, Rodney P.; Tercero, Napoleon; Qiao, Wanqiong; Levicky, Rastislav

doi:10.1149/1.3571981

Cited by 3 publications

(2 citation statements)

References 29 publications

(59 reference statements)

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“…Such an explanation, however, is inconsistent with MCH-passivated electrodes typically exhibiting negative potentials of zero charge (pzc), , what implies MCH supports to have a positive charge at the 0 V used for hybridization. Our own measurements, based on minimum in interfacial capacitance, confirmed that pzc is negative for MCH-passivated electrodes bearing unhybridized MO probes under pH 7.0 sodium phosphate, with pzc falling between −0.1 and −0.2 V . Therefore, some other, as yet unidentified effect is believed responsible for the dependence of k F on C B .…”

Section: Resultssupporting

confidence: 62%

Kinetic Mechanisms in Morpholino–DNA Surface Hybridization

et al. 2011

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Morpholinos (MOs) are DNA analogues whose uncharged nature can bring fundamental advantages to surface hybridization technologies such as DNA microarrays, by using MOs as the immobilized, or “probe”, species. Advancement of MO-based diagnostics, however, is challenged by limited understanding of the surface organization of MO molecules and of how this organization impacts hybridization kinetics and thermodynamics. The present study focuses on hybridization kinetics between monolayers of MO probes and DNA targets as a function of the instantaneous extent of hybridization (i.e. duplex coverage), total probe coverage, and ionic strength. Intriguingly, these experiments reveal distinct kinetic stages, none of which are consistent with Langmuir kinetics. The initial stage, in which duplex coverage remains relatively sparse, indicates confluence of two effects: blockage of target access to unhybridized probes by previously formed duplexes, and deactivation of the solid support due to consumption of probe molecules. This interpretation is consistent with a surface organization in which unhybridized MO probes localize near the solid support, underneath a layer of MO-DNA duplexes. As duplex coverage builds, provided saturation is not reached first, the initial stage can transition to an unusual regime characterized by near independence of hybridization rate on duplex coverage, followed by a prolonged approach to equilibrium. The possible origins of these more complex latter behaviors are discussed. Comparison with published data for DNA and peptide nucleic acid (PNA) probes is carried out to look for universal trends in kinetics. This comparison reveals qualitative similarities when comparable surface organization of probes is expected. In addition, MO monolayers are found capable of a broad range of reactivities that span reported values for PNA and DNA probes.

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Section: Resultssupporting

confidence: 62%

Kinetic Mechanisms in Morpholino–DNA Surface Hybridization

et al. 2011

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“…31 The increased rigidity of the backbone also reduces self-hybridisation. The benefits of MOs have been assessed in electrochemical studies 32,33 and microarrays 34 but have hitherto been unused in responsive hydrogels or in other fields using oligonucleotide interactions such as DNA nanotechnology.…”

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confidence: 99%

Morpholino Oligonucleotide Cross-Linked Hydrogels as Portable Optical Oligonucleotide Biosensors

et al. 2018

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Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive cross-links for microRNA sequence detection. The MO cross-links can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO cross-links offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.

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Functional Molecular Interfaces for Impedance-Based Diagnostics

Kanyong

Patil

Davis

2020

Annual Rev. Anal. Chem.

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In seeking to develop and optimize reagentless electroanalytical assays, a consideration of the transducing interface features lies key to any subsequent sensitivity and selectivity. This review briefly summarizes some of the most commonly used receptive interfaces that have been employed within the development of impedimetric molecular sensors. We discuss the use of high surface area carbon, nanoparticles, and a range of bioreceptors that can subsequently be integrated. The review spans the most commonly utilized biorecognition elements, such as antibodies, antibody fragments, aptamers, and nucleic acids, and touches on some novel emerging alternatives such as nanofragments, molecularly imprinted polymers, and bacteriophages. Reference is made to the immobilization chemistries available along with a consideration of both optimal packing density and recognition probe orientation. We also discuss assay-relevant mechanistic details and applications in real sample analysis.

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Electrochemical Studies of Morpholino-DNA Surface Hybridization

Cited by 3 publications

References 29 publications

Kinetic Mechanisms in Morpholino–DNA Surface Hybridization

Kinetic Mechanisms in Morpholino–DNA Surface Hybridization

Morpholino Oligonucleotide Cross-Linked Hydrogels as Portable Optical Oligonucleotide Biosensors

Functional Molecular Interfaces for Impedance-Based Diagnostics

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