Electrochemical strategy for ultrasensitive detection of microRNA based on MNAzyme-mediated rolling circle amplification on a gold electrode

Yang, Jianru; Tang, Min; Diao, Wei; Cheng, Wei; Zhang, Ye; Yan, Yurong

doi:10.1007/s00604-016-1958-5

Cited by 59 publications

(14 citation statements)

References 33 publications

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“…For example, Yang et al. [25] described an electrochemical strategy for ultrasensitive and specific detection of microRNA based on multicomponent nucleic acid enzyme-mediated rolling circle amplification on a gold electrode. Hong et al.…”

Section: Newly Developed Methodsmentioning

confidence: 99%

“…Traditional methods for miRNA detection include northern blotting [[10], [11], [12]], microarray analysis [13,14] and quantitative polymerase chain reaction (qPCR) [15,16]. For new technology methods, in order to improve the sensitivity and selectivity of miRNA detection, these methods always rely on signal amplification strategies, such as nanoparticle-based amplification [[17], [18], [19], [20], [21]], isothermal exponential amplification [[22], [23], [24]], rolling circle amplification (RCA) [[25], [26], [27]], hybridization chain reaction (HCR) [[28], [29], [30]] and combination of these amplification strategies [[31], [32], [33]]. Herein, we summarize some of them and discuss their advantages and limitations for improving miRNA detection design.…”

Section: Introductionmentioning

confidence: 99%

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Research advances in the detection of miRNA

Tian

et al. 2019

Journal of Pharmaceutical Analysis

269

152

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MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attracted significant interest, as abnormal miRNA expression is identified to contribute to serious human diseases such as cancers. Traditional methods for miRNA detection do not meet current demands. In particular, nanomaterial-based methods, nucleic acid amplification-based methods such as rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), strand-displacement amplification (SDA) and some enzyme-free amplifications have been employed widely for the highly sensitive detection of miRNA. MiRNA functional research and clinical diagnostics have been accelerated by these new techniques. Herein, we summarize and discuss the recent progress in the development of miRNA detection methods and new applications. This review will provide guidelines for the development of follow-up miRNA detection methods with high sensitivity and specificity, and applicability to disease diagnosis and therapy.

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Section: Newly Developed Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Research advances in the detection of miRNA

Tian

et al. 2019

Journal of Pharmaceutical Analysis

269

152

View full text Add to dashboard Cite

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“…We demonstrated rational combination of LNA modification, S-arm truncation and the addition of copolymer which synergistically enhanced MNAzymes activity and selectivity greater than the sum of the effect from each strategy. MNAzyme-based assay generally couples with complicated signal amplification strategies to enhance analytical performance ( Li et al, 2016 ; Yang et al, 2016 ). In contrast, our strategy permits sensitive, one-pot RNA detection without labor-intensive steps.…”

Section: Introductionmentioning

confidence: 99%

One-step isothermal RNA detection with LNA-modified MNAzymes chaperoned by cationic copolymer

Hanpanich

Saito

Shimada

et al. 2020

Biosensors and Bioelectronics

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RNA detection permits early diagnosis of several infectious diseases and cancers, which prevent propagation of diseases and improve treatment efficacy. However, standard technique for RNA detection such as reverse transcription-quantitative polymerase chain reaction has complicated procedure and requires well-trained personnel and specialized lab equipment. These shortcomings limit the application for point-of-care analysis which is critical for rapid and effective disease management. The multicomponent nucleic acid enzymes (MNAzymes) are one of the promising biosensors for simple, isothermal and enzyme-free RNA detection. Herein, we demonstrate simple yet effective strategies that significantly enhance analytical performance of MNAzymes. The addition of the cationic copolymer and structural modification of MNAzyme significantly enhanced selectivity and activity of MNAzymes by 250 fold and 2,700 fold, respectively. The highly simplified RNA detection system achieved a detection limit of 73 fM target concentration without additional amplification. The robustness of MNAzyme in the presence of non-target RNA was also improved. Our finding opens up a route toward the development of an alternative rapid, sensitive, isothermal, and protein-free RNA diagnostic tool, which expected to be of great clinical significance.

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“…Certainly, other various techniques have been developed, including electrochemical sensor, surface plasmon resonance (SPR), surface-enhanced Raman scattering (SERS), colorimetry and fluorescence [ 10 – 14 ]. Among the various techniques, fluorescence detection platform has emerged as an excellent alternative for the detection, quantification and characterization of target [ 15 – 17 ]. Organic fluorescent dye-labeled DNA probe and fluorescent technique have been widely employed for ultrasensitive quantification of DNA, miRNA, proteins, and others because of the easily commercialized synthesis [ 18 – 20 ].…”

Section: Introductionmentioning

confidence: 99%

A novel quantification platform for point-of-care testing of circulating MicroRNAs based on allosteric spherical nanoprobe

et al. 2020

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MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer (NSCLC), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC. Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield “signal on” fluorescence. In the manner of multipoint fluorescence detection, the target-bound allosteric spherical nanoprobe could provide high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method can distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

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Electrochemical strategy for ultrasensitive detection of microRNA based on MNAzyme-mediated rolling circle amplification on a gold electrode

Cited by 59 publications

References 33 publications

Research advances in the detection of miRNA

Research advances in the detection of miRNA

One-step isothermal RNA detection with LNA-modified MNAzymes chaperoned by cationic copolymer

A novel quantification platform for point-of-care testing of circulating MicroRNAs based on allosteric spherical nanoprobe

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